Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells

Qingyong Li, Chen Liu, Xiuhua Zhao, Yuangang Zu, Ying Wang, Baoyou Zhang, Dongmei Zhao, Qi Zhao, Lin Su, Yang Gao, Baihe SunKey Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, Heilongjiang, People&#39;s Republic of ChinaBackground: The purpos...

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Autores principales:	et al, Xiuhua Zhao, Qingyong Li, Chen Liu
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/750b5602c5a24f87a760d130e3d41e70
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spelling	oai:doaj.org-article:750b5602c5a24f87a760d130e3d41e702021-12-02T06:22:50ZPreparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells1176-91141178-2013https://doaj.org/article/750b5602c5a24f87a760d130e3d41e702011-02-01T00:00:00Zhttp://www.dovepress.com/preparation-characterization-and-targeting-of-micronized-10-hydroxycam-a6393https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Qingyong Li, Chen Liu, Xiuhua Zhao, Yuangang Zu, Ying Wang, Baoyou Zhang, Dongmei Zhao, Qi Zhao, Lin Su, Yang Gao, Baihe SunKey Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, Heilongjiang, People&#39;s Republic of ChinaBackground: The purpose of this study was to develop a method for targeted delivery of 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) to cancer cells.Methods: We first used a supercritical antisolvent process to prepare micronized HCPT (nHCPT), and then folate-conjugated human serum albumin (HSA) nHCPT-loaded NPs (FA-HSA-nHCPT-NPs) were prepared using a NP-coated method combined with a desolvation technique. The amount of folate conjugation was 16 &micro;g &middot; mg-1 HSA.Results: The particle size of the spherical nHCPT microparticles obtained was 118.5 &plusmn; 6.6 nm. The particle size and zeta potential of the FA-HSA-nHCPT-NPs were 233.9 &plusmn; 1.2 nm and -25.23 &plusmn; 2.98 mV, respectively. The FA-HSA-nHCPT-NPs exhibited a smooth surface and a distinct spherical shape, and the results of differential scanning calorimetry and X-ray diffraction indicated that the FA-HSA-nHCPT-NPs presented in a nanostructured amorphous state. The FA-HSA-nHCPT-NPs showed sustained-release characteristics for 120 hours in vitro, with a drug-loading content of 7.3% and an encapsulating efficiency of 79.1%.Conclusion: The FA-NPs were effective delivery systems for uptake by SGC7901 cells compared with folate-free NPs. These results suggest that a NP-coated method combined with a desolvation technique is effective for preparing NPs with drugs having poor solubility in water and most organic solvents, using albumin as the wall material. FA-HSA-NPs are a stable delivery system and have the potential for targeted delivery of anticancer drugs.Keywords: nanoparticle-coated, desolvation technique, 10-hydroxycamptothecin, human serum albumin, folate, targeted delivery&nbsp; et alXiuhua ZhaoQingyong LiChen LiuDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 397-405 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 et al Xiuhua Zhao Qingyong Li Chen Liu Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells
description	Qingyong Li, Chen Liu, Xiuhua Zhao, Yuangang Zu, Ying Wang, Baoyou Zhang, Dongmei Zhao, Qi Zhao, Lin Su, Yang Gao, Baihe SunKey Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, Heilongjiang, People&#39;s Republic of ChinaBackground: The purpose of this study was to develop a method for targeted delivery of 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) to cancer cells.Methods: We first used a supercritical antisolvent process to prepare micronized HCPT (nHCPT), and then folate-conjugated human serum albumin (HSA) nHCPT-loaded NPs (FA-HSA-nHCPT-NPs) were prepared using a NP-coated method combined with a desolvation technique. The amount of folate conjugation was 16 &micro;g &middot; mg-1 HSA.Results: The particle size of the spherical nHCPT microparticles obtained was 118.5 &plusmn; 6.6 nm. The particle size and zeta potential of the FA-HSA-nHCPT-NPs were 233.9 &plusmn; 1.2 nm and -25.23 &plusmn; 2.98 mV, respectively. The FA-HSA-nHCPT-NPs exhibited a smooth surface and a distinct spherical shape, and the results of differential scanning calorimetry and X-ray diffraction indicated that the FA-HSA-nHCPT-NPs presented in a nanostructured amorphous state. The FA-HSA-nHCPT-NPs showed sustained-release characteristics for 120 hours in vitro, with a drug-loading content of 7.3% and an encapsulating efficiency of 79.1%.Conclusion: The FA-NPs were effective delivery systems for uptake by SGC7901 cells compared with folate-free NPs. These results suggest that a NP-coated method combined with a desolvation technique is effective for preparing NPs with drugs having poor solubility in water and most organic solvents, using albumin as the wall material. FA-HSA-NPs are a stable delivery system and have the potential for targeted delivery of anticancer drugs.Keywords: nanoparticle-coated, desolvation technique, 10-hydroxycamptothecin, human serum albumin, folate, targeted delivery&nbsp;
format	article
author	et al Xiuhua Zhao Qingyong Li Chen Liu
author_facet	et al Xiuhua Zhao Qingyong Li Chen Liu
author_sort	et al
title	Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells
title_short	Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells
title_full	Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells
title_fullStr	Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells
title_full_unstemmed	Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells
title_sort	preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/750b5602c5a24f87a760d130e3d41e70
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Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells

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