Enhancement of Radiosensitization by Silver Nanoparticles Functionalized with Polyethylene Glycol and Aptamer As1411 for Glioma Irradiation Therapy

Jing Zhao,1 Peidang Liu,1,2 Jun Ma,3 Dongdong Li,1 Huiquan Yang,1 Wenbin Chen,1 Yaowen Jiang2 1School of Medicine, Southeast University, Nanjing 210009, People’s Republic of China; 2Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing 210096, People’...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhao J, Liu P, Ma J, Li D, Yang H, Chen W, Jiang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	radiosensitization as1411 silver nanoparticles glioma Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/750ec6cd0e9a4a818a08d6e1cfa3ff8c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:750ec6cd0e9a4a818a08d6e1cfa3ff8c
record_format	dspace
spelling	oai:doaj.org-article:750ec6cd0e9a4a818a08d6e1cfa3ff8c2021-12-02T03:56:00ZEnhancement of Radiosensitization by Silver Nanoparticles Functionalized with Polyethylene Glycol and Aptamer As1411 for Glioma Irradiation Therapy1178-2013https://doaj.org/article/750ec6cd0e9a4a818a08d6e1cfa3ff8c2019-12-01T00:00:00Zhttps://www.dovepress.com/enhancement-of-radiosensitization-by-silver-nanoparticles-functionaliz-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jing Zhao,1 Peidang Liu,1,2 Jun Ma,3 Dongdong Li,1 Huiquan Yang,1 Wenbin Chen,1 Yaowen Jiang2 1School of Medicine, Southeast University, Nanjing 210009, People’s Republic of China; 2Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing 210096, People’s Republic of China; 3Radiotherapy Department, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, People’s Republic of ChinaCorrespondence: Peidang LiuSchool of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing 210009, Jiangsu Province, People’s Republic of ChinaTel/Fax +86 25 8327 2554Email seulpd@163.comBackground: The efficacy of radiotherapy for glioma is often limited by the radioresistance of glioma cells. The radiosensitizing effects of silver nanoparticles (AgNPs) on glioma were found in the previous studies of our group. In order to enhance the radiosensitivity of tumor cells and selectively kill them while reducing the side effects of irradiation therapy, targeted modification of AgNPs is urgently needed.Materials and methods: In the present study, AgNPs functionalized with polyethylene glycol (PEG) and aptamer As1411 (AsNPs) were synthesized and subsequently characterized by transmission electron microscopy, ultraviolet-visible spectroscopy and Fourier transform infrared spectroscopy. Then the targeting property of AsNPs was evaluated by dark-field imaging, confocal microscopy and in vivo imaging. Both colony formation assay and glioma-bearing mouse model were employed to study the radiosensitizing effect of AsNPs.Results: The characterization results revealed a spherical shape of AgNPs with an average diameter of 18 nm and the successful construction of AsNPs. AsNPs were confirmed to specifically target C6 glioma cells, but not normal human microvascular endothelial cells. Moreover, AsNPs could not only internalize into tumor cells, but also penetrate into the core of tumor spheroids. In vitro experiments showed that AsNPs exhibited a better radiosensitizing effect than AgNPs and PEGylated AgNPs (PNPs), inducing a higher rate of apoptotic cell death. In vivo imaging demonstrated that Cy5-AsNPs preferentially accumulated at the tumor site, and the ratio of fluorescence intensity of Cy5-AsNPs to that of Cy5-PNPs reached the maximum at 6 h post-systemic administration. Furthermore, the combination of AsNPs with irradiation significantly prolonged the median survival time of C6 glioma-bearing mice.Conclusion: Our results indicated that AsNPs could be an effective nano-radiosensitizer for glioma targeting treatment.Keywords: radiosensitization, As1411, silver nanoparticles, gliomaZhao JLiu PMa JLi DYang HChen WJiang YDove Medical Pressarticleradiosensitizationas1411silver nanoparticlesgliomaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9483-9496 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	radiosensitization as1411 silver nanoparticles glioma Medicine (General) R5-920
spellingShingle	radiosensitization as1411 silver nanoparticles glioma Medicine (General) R5-920 Zhao J Liu P Ma J Li D Yang H Chen W Jiang Y Enhancement of Radiosensitization by Silver Nanoparticles Functionalized with Polyethylene Glycol and Aptamer As1411 for Glioma Irradiation Therapy
description	Jing Zhao,1 Peidang Liu,1,2 Jun Ma,3 Dongdong Li,1 Huiquan Yang,1 Wenbin Chen,1 Yaowen Jiang2 1School of Medicine, Southeast University, Nanjing 210009, People’s Republic of China; 2Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing 210096, People’s Republic of China; 3Radiotherapy Department, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, People’s Republic of ChinaCorrespondence: Peidang LiuSchool of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing 210009, Jiangsu Province, People’s Republic of ChinaTel/Fax +86 25 8327 2554Email seulpd@163.comBackground: The efficacy of radiotherapy for glioma is often limited by the radioresistance of glioma cells. The radiosensitizing effects of silver nanoparticles (AgNPs) on glioma were found in the previous studies of our group. In order to enhance the radiosensitivity of tumor cells and selectively kill them while reducing the side effects of irradiation therapy, targeted modification of AgNPs is urgently needed.Materials and methods: In the present study, AgNPs functionalized with polyethylene glycol (PEG) and aptamer As1411 (AsNPs) were synthesized and subsequently characterized by transmission electron microscopy, ultraviolet-visible spectroscopy and Fourier transform infrared spectroscopy. Then the targeting property of AsNPs was evaluated by dark-field imaging, confocal microscopy and in vivo imaging. Both colony formation assay and glioma-bearing mouse model were employed to study the radiosensitizing effect of AsNPs.Results: The characterization results revealed a spherical shape of AgNPs with an average diameter of 18 nm and the successful construction of AsNPs. AsNPs were confirmed to specifically target C6 glioma cells, but not normal human microvascular endothelial cells. Moreover, AsNPs could not only internalize into tumor cells, but also penetrate into the core of tumor spheroids. In vitro experiments showed that AsNPs exhibited a better radiosensitizing effect than AgNPs and PEGylated AgNPs (PNPs), inducing a higher rate of apoptotic cell death. In vivo imaging demonstrated that Cy5-AsNPs preferentially accumulated at the tumor site, and the ratio of fluorescence intensity of Cy5-AsNPs to that of Cy5-PNPs reached the maximum at 6 h post-systemic administration. Furthermore, the combination of AsNPs with irradiation significantly prolonged the median survival time of C6 glioma-bearing mice.Conclusion: Our results indicated that AsNPs could be an effective nano-radiosensitizer for glioma targeting treatment.Keywords: radiosensitization, As1411, silver nanoparticles, glioma
format	article
author	Zhao J Liu P Ma J Li D Yang H Chen W Jiang Y
author_facet	Zhao J Liu P Ma J Li D Yang H Chen W Jiang Y
author_sort	Zhao J
title	Enhancement of Radiosensitization by Silver Nanoparticles Functionalized with Polyethylene Glycol and Aptamer As1411 for Glioma Irradiation Therapy
title_short	Enhancement of Radiosensitization by Silver Nanoparticles Functionalized with Polyethylene Glycol and Aptamer As1411 for Glioma Irradiation Therapy
title_full	Enhancement of Radiosensitization by Silver Nanoparticles Functionalized with Polyethylene Glycol and Aptamer As1411 for Glioma Irradiation Therapy
title_fullStr	Enhancement of Radiosensitization by Silver Nanoparticles Functionalized with Polyethylene Glycol and Aptamer As1411 for Glioma Irradiation Therapy
title_full_unstemmed	Enhancement of Radiosensitization by Silver Nanoparticles Functionalized with Polyethylene Glycol and Aptamer As1411 for Glioma Irradiation Therapy
title_sort	enhancement of radiosensitization by silver nanoparticles functionalized with polyethylene glycol and aptamer as1411 for glioma irradiation therapy
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/750ec6cd0e9a4a818a08d6e1cfa3ff8c
work_keys_str_mv	AT zhaoj enhancementofradiosensitizationbysilvernanoparticlesfunctionalizedwithpolyethyleneglycolandaptameras1411forgliomairradiationtherapy AT liup enhancementofradiosensitizationbysilvernanoparticlesfunctionalizedwithpolyethyleneglycolandaptameras1411forgliomairradiationtherapy AT maj enhancementofradiosensitizationbysilvernanoparticlesfunctionalizedwithpolyethyleneglycolandaptameras1411forgliomairradiationtherapy AT lid enhancementofradiosensitizationbysilvernanoparticlesfunctionalizedwithpolyethyleneglycolandaptameras1411forgliomairradiationtherapy AT yangh enhancementofradiosensitizationbysilvernanoparticlesfunctionalizedwithpolyethyleneglycolandaptameras1411forgliomairradiationtherapy AT chenw enhancementofradiosensitizationbysilvernanoparticlesfunctionalizedwithpolyethyleneglycolandaptameras1411forgliomairradiationtherapy AT jiangy enhancementofradiosensitizationbysilvernanoparticlesfunctionalizedwithpolyethyleneglycolandaptameras1411forgliomairradiationtherapy
_version_	1718401557815361536

Enhancement of Radiosensitization by Silver Nanoparticles Functionalized with Polyethylene Glycol and Aptamer As1411 for Glioma Irradiation Therapy

Ejemplares similares