Histological assessment of PAXgene tissue fixation and stabilization reagents.
Within SPIDIA, an EC FP7 project aimed to improve pre analytic procedures, the PAXgene Tissue System (PAXgene), was designed to improve tissue quality for parallel molecular and morphological analysis. Within the SPIDIA project promising results were found in both genomic and proteomic experiments w...
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2011
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oai:doaj.org-article:75166d8670a14e3cb3e15d6db17fc7ce2021-11-18T07:34:07ZHistological assessment of PAXgene tissue fixation and stabilization reagents.1932-620310.1371/journal.pone.0027704https://doaj.org/article/75166d8670a14e3cb3e15d6db17fc7ce2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22110732/?tool=EBIhttps://doaj.org/toc/1932-6203Within SPIDIA, an EC FP7 project aimed to improve pre analytic procedures, the PAXgene Tissue System (PAXgene), was designed to improve tissue quality for parallel molecular and morphological analysis. Within the SPIDIA project promising results were found in both genomic and proteomic experiments with PAXgene-fixed and paraffin embedded tissue derived biomolecules. But, for this technology to be accepted for use in both clinical and basic research, it is essential that its adequacy for preserving morphology and antigenicity is validated relative to formalin fixation. It is our aim to assess the suitability of PAXgene tissue fixation for (immuno)histological methods. Normal human tissue specimens (n = 70) were collected and divided into equal parts for fixation either with formalin or PAXgene. Sections of the obtained paraffin-embedded tissue were cut and stained. Morphological aspects of PAXgene-fixed tissue were described and also scored relative to formalin-fixed tissue. Performance of PAXgene-fixed tissue in immunohistochemical and in situ hybridization assays was also assessed relative to the corresponding formalin-fixed tissues. Morphology of PAXgene-fixed paraffin embedded tissue was well preserved and deemed adequate for diagnostics in most cases. Some antigens in PAXgene-fixed and paraffin embedded sections were detectable without the need for antigen retrieval, while others were detected using standard, formalin fixation based, immunohistochemistry protocols. Comparable results were obtained with in situ hybridization and histochemical stains. Basically all assessed histological techniques were found to be applicable to PAXgene-fixed and paraffin embedded tissue. In general results obtained with PAXgene-fixed tissue are comparable to those of formalin-fixed tissue. Compromises made in morphology can be called minor compared to the advantages in the molecular pathology possibilities.Marcel KapFrank SmedtsWolter OosterhuisRosa WintherNanna ChristensenBilge ReischauerChristian ViertlerDaniel GroelzKarl-Friedrich BeckerKurt ZatloukalRupert LangerJulia Slotta-HuspeninaKoppany BodoBas de JongUwe OelmullerPeter RiegmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27704 (2011) |
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Medicine R Science Q Marcel Kap Frank Smedts Wolter Oosterhuis Rosa Winther Nanna Christensen Bilge Reischauer Christian Viertler Daniel Groelz Karl-Friedrich Becker Kurt Zatloukal Rupert Langer Julia Slotta-Huspenina Koppany Bodo Bas de Jong Uwe Oelmuller Peter Riegman Histological assessment of PAXgene tissue fixation and stabilization reagents. |
| description |
Within SPIDIA, an EC FP7 project aimed to improve pre analytic procedures, the PAXgene Tissue System (PAXgene), was designed to improve tissue quality for parallel molecular and morphological analysis. Within the SPIDIA project promising results were found in both genomic and proteomic experiments with PAXgene-fixed and paraffin embedded tissue derived biomolecules. But, for this technology to be accepted for use in both clinical and basic research, it is essential that its adequacy for preserving morphology and antigenicity is validated relative to formalin fixation. It is our aim to assess the suitability of PAXgene tissue fixation for (immuno)histological methods. Normal human tissue specimens (n = 70) were collected and divided into equal parts for fixation either with formalin or PAXgene. Sections of the obtained paraffin-embedded tissue were cut and stained. Morphological aspects of PAXgene-fixed tissue were described and also scored relative to formalin-fixed tissue. Performance of PAXgene-fixed tissue in immunohistochemical and in situ hybridization assays was also assessed relative to the corresponding formalin-fixed tissues. Morphology of PAXgene-fixed paraffin embedded tissue was well preserved and deemed adequate for diagnostics in most cases. Some antigens in PAXgene-fixed and paraffin embedded sections were detectable without the need for antigen retrieval, while others were detected using standard, formalin fixation based, immunohistochemistry protocols. Comparable results were obtained with in situ hybridization and histochemical stains. Basically all assessed histological techniques were found to be applicable to PAXgene-fixed and paraffin embedded tissue. In general results obtained with PAXgene-fixed tissue are comparable to those of formalin-fixed tissue. Compromises made in morphology can be called minor compared to the advantages in the molecular pathology possibilities. |
| format |
article |
| author |
Marcel Kap Frank Smedts Wolter Oosterhuis Rosa Winther Nanna Christensen Bilge Reischauer Christian Viertler Daniel Groelz Karl-Friedrich Becker Kurt Zatloukal Rupert Langer Julia Slotta-Huspenina Koppany Bodo Bas de Jong Uwe Oelmuller Peter Riegman |
| author_facet |
Marcel Kap Frank Smedts Wolter Oosterhuis Rosa Winther Nanna Christensen Bilge Reischauer Christian Viertler Daniel Groelz Karl-Friedrich Becker Kurt Zatloukal Rupert Langer Julia Slotta-Huspenina Koppany Bodo Bas de Jong Uwe Oelmuller Peter Riegman |
| author_sort |
Marcel Kap |
| title |
Histological assessment of PAXgene tissue fixation and stabilization reagents. |
| title_short |
Histological assessment of PAXgene tissue fixation and stabilization reagents. |
| title_full |
Histological assessment of PAXgene tissue fixation and stabilization reagents. |
| title_fullStr |
Histological assessment of PAXgene tissue fixation and stabilization reagents. |
| title_full_unstemmed |
Histological assessment of PAXgene tissue fixation and stabilization reagents. |
| title_sort |
histological assessment of paxgene tissue fixation and stabilization reagents. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/75166d8670a14e3cb3e15d6db17fc7ce |
| work_keys_str_mv |
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1718423293648699392 |