ASSESMENT OF PLTELET ANTIAGGREGANT ACTIVITY OF A FRACTION FROM AN ETHANOLIC EXTRACT OF THE BARK OF Nectandra amazonum Nees

ABSTRACT Background: Some species of the Lauraceae family are known to produce secondary metabolites that have antiplatelet properties. Studies on the leaves of Nectandra amazonum Nees. have shown antiaggregant activity but the bark of this species has not been studied. Objectives: To assess the a...

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Autores principales:	Jenaro Antonio ESPITIA CORREDOR, Luis Enrique CUCA SUÁREZ, Mario Francisco GUERRERO PABÓN
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia
Materias:	Lauraceae platelet aggregation arachidonic acid adenosine diphosphate epinephrine collagen Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/751990923f624ffc951516d6d072c6b5
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spelling	oai:doaj.org-article:751990923f624ffc951516d6d072c6b52021-12-02T10:39:24ZASSESMENT OF PLTELET ANTIAGGREGANT ACTIVITY OF A FRACTION FROM AN ETHANOLIC EXTRACT OF THE BARK OF Nectandra amazonum Nees0121-400410.17533/udea.vitae.v23n2a04https://doaj.org/article/751990923f624ffc951516d6d072c6b5http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-40042016000200119&lng=en&tlng=enhttps://doaj.org/toc/0121-4004ABSTRACT Background: Some species of the Lauraceae family are known to produce secondary metabolites that have antiplatelet properties. Studies on the leaves of Nectandra amazonum Nees. have shown antiaggregant activity but the bark of this species has not been studied. Objectives: To assess the antiplatelet effect of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees. (N.V. “laurel amarillo”, Lauraceae) applying the “Born” turbidimetric method. Methods: The screening test compared the effects of a fraction of N. amazonum (0.1 mg/mL), acetylsalicylic acid (ASA, 0.5 mM, as reference standard) and dimethylsulphoxide (DMSO, 0.1%, as control) on human platelets stimulated with adenosine diphosphate (ADP, 2 μM), epinephrine (EPI, 1 uM (one micromolar)), collagen (COLL, 1 μg/mL) and arachidonic acid (AA, 0.2 mg/mL). Subsequently, the study focused on determining the antiaggregant potency of the N. amazonum fraction through concentration - response curves (from 1 μg/mL to 0.4 mg/mL), obtaining pIC50 (-log IC50) values against the platelet agonists. Results: Control platelets attained the highest percentage values of aggregation (96% AA, 89% EPI, 85% COLL, and 77% ADP). The N. amazonum fraction significantly reduced the aggregation effects (6% AA, 45% EPI, 10% COLL, 21% ADP), with values close to those obtained with ASA (17% AA, 21% EPI, 10% COLL, 20% ADP). According to concentration - response curves, the pIC50 values of the ethanolic fraction indicated the following order of potency: AA, 4.90 > ADP, 4.51 > COLL, 4.33 > EPI, 3.85. Conclusions: These results suggest that the N. amazonum Nees. ethanolic fraction elicited antiplatelet effects mainly related to the inhibition of the arachidonic acid pathway.Jenaro Antonio ESPITIA CORREDORLuis Enrique CUCA SUÁREZMario Francisco GUERRERO PABÓNUniversidad de AntioquiaarticleLauraceaeplatelet aggregationarachidonic acidadenosine diphosphateepinephrinecollagenFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 23, Iss 2, Pp 119-123
institution	DOAJ
collection	DOAJ
language	EN
topic	Lauraceae platelet aggregation arachidonic acid adenosine diphosphate epinephrine collagen Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Lauraceae platelet aggregation arachidonic acid adenosine diphosphate epinephrine collagen Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Jenaro Antonio ESPITIA CORREDOR Luis Enrique CUCA SUÁREZ Mario Francisco GUERRERO PABÓN ASSESMENT OF PLTELET ANTIAGGREGANT ACTIVITY OF A FRACTION FROM AN ETHANOLIC EXTRACT OF THE BARK OF Nectandra amazonum Nees
description	ABSTRACT Background: Some species of the Lauraceae family are known to produce secondary metabolites that have antiplatelet properties. Studies on the leaves of Nectandra amazonum Nees. have shown antiaggregant activity but the bark of this species has not been studied. Objectives: To assess the antiplatelet effect of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees. (N.V. “laurel amarillo”, Lauraceae) applying the “Born” turbidimetric method. Methods: The screening test compared the effects of a fraction of N. amazonum (0.1 mg/mL), acetylsalicylic acid (ASA, 0.5 mM, as reference standard) and dimethylsulphoxide (DMSO, 0.1%, as control) on human platelets stimulated with adenosine diphosphate (ADP, 2 μM), epinephrine (EPI, 1 uM (one micromolar)), collagen (COLL, 1 μg/mL) and arachidonic acid (AA, 0.2 mg/mL). Subsequently, the study focused on determining the antiaggregant potency of the N. amazonum fraction through concentration - response curves (from 1 μg/mL to 0.4 mg/mL), obtaining pIC50 (-log IC50) values against the platelet agonists. Results: Control platelets attained the highest percentage values of aggregation (96% AA, 89% EPI, 85% COLL, and 77% ADP). The N. amazonum fraction significantly reduced the aggregation effects (6% AA, 45% EPI, 10% COLL, 21% ADP), with values close to those obtained with ASA (17% AA, 21% EPI, 10% COLL, 20% ADP). According to concentration - response curves, the pIC50 values of the ethanolic fraction indicated the following order of potency: AA, 4.90 > ADP, 4.51 > COLL, 4.33 > EPI, 3.85. Conclusions: These results suggest that the N. amazonum Nees. ethanolic fraction elicited antiplatelet effects mainly related to the inhibition of the arachidonic acid pathway.
format	article
author	Jenaro Antonio ESPITIA CORREDOR Luis Enrique CUCA SUÁREZ Mario Francisco GUERRERO PABÓN
author_facet	Jenaro Antonio ESPITIA CORREDOR Luis Enrique CUCA SUÁREZ Mario Francisco GUERRERO PABÓN
author_sort	Jenaro Antonio ESPITIA CORREDOR
title	ASSESMENT OF PLTELET ANTIAGGREGANT ACTIVITY OF A FRACTION FROM AN ETHANOLIC EXTRACT OF THE BARK OF Nectandra amazonum Nees
title_short	ASSESMENT OF PLTELET ANTIAGGREGANT ACTIVITY OF A FRACTION FROM AN ETHANOLIC EXTRACT OF THE BARK OF Nectandra amazonum Nees
title_full	ASSESMENT OF PLTELET ANTIAGGREGANT ACTIVITY OF A FRACTION FROM AN ETHANOLIC EXTRACT OF THE BARK OF Nectandra amazonum Nees
title_fullStr	ASSESMENT OF PLTELET ANTIAGGREGANT ACTIVITY OF A FRACTION FROM AN ETHANOLIC EXTRACT OF THE BARK OF Nectandra amazonum Nees
title_full_unstemmed	ASSESMENT OF PLTELET ANTIAGGREGANT ACTIVITY OF A FRACTION FROM AN ETHANOLIC EXTRACT OF THE BARK OF Nectandra amazonum Nees
title_sort	assesment of pltelet antiaggregant activity of a fraction from an ethanolic extract of the bark of nectandra amazonum nees
publisher	Universidad de Antioquia
url	https://doaj.org/article/751990923f624ffc951516d6d072c6b5
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ASSESMENT OF PLTELET ANTIAGGREGANT ACTIVITY OF A FRACTION FROM AN ETHANOLIC EXTRACT OF THE BARK OF Nectandra amazonum Nees

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