Effect of Ruta Graveolens Hydro-Alcoholic Extract on Pentylenetetrazole-Induced Seizure in Male Mice

BACKGROUND AND OBJECTIVE: Seizure is an important symptom of epilepsy and many other neurogenic disorders. Despite a variety of current anti-convulsive drugs, research for discovering new drugs with more efficacy and less unsuitable adverse effects has been continued. Herbal medicine has various nat...

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Autores principales: F Keihanian, M Rostampour VajariA, A Saeidynia, AR Elmieh
Formato: article
Lenguaje:EN
FA
Publicado: Babol University of Medical Sciences 2012
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Acceso en línea:https://doaj.org/article/7528386db2074c64b439ec0fedb565bc
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spelling oai:doaj.org-article:7528386db2074c64b439ec0fedb565bc2021-11-10T08:53:19ZEffect of Ruta Graveolens Hydro-Alcoholic Extract on Pentylenetetrazole-Induced Seizure in Male Mice1561-41072251-7170https://doaj.org/article/7528386db2074c64b439ec0fedb565bc2012-07-01T00:00:00Zhttp://jbums.org/article-1-4146-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Seizure is an important symptom of epilepsy and many other neurogenic disorders. Despite a variety of current anti-convulsive drugs, research for discovering new drugs with more efficacy and less unsuitable adverse effects has been continued. Herbal medicine has various natural substances and proper context for this type of research. The aim of this study was to survey the anticonvulsant effects of hydro-alcoholic extract of rue (Ruta graveolens) in male mice.METHODS: In this experimental study anticonvulsant effect of extract evaluated by PTZ induced seizure. Fifty-six NMRI mice in the range of 25-35 gr were divided to 7 groups of 8 mice that included five cases, one positive control group and one negative control group. Case groups have been injected by 100, 300, 500, 800 and 1000 mg/kg of extracts, positive control group 40 ml/kg phenobarbital and negative control group 10 ml/kg normal saline intraperitoneally. All groups were injected by pentylenetetrazole (80mg/kg) intraperitoneally after 45 minutes, and initiation time of myocolonic and tonic-colonic seizures and rate of 24 hours death were measured.FINDINGS: Consequences of different doses of rue hydro-alcoholic extract increased delay in initiation of myocolonic and tonic-colonic seizures rather than control group dose-dependently and reduced 24 hours seizure-induced mortality (p<0.05). Delay in myocolonic seizures in doses of 300, 500, 800 and 1000 mg/kg (p<0.001) and 100 mg/kg (p<0.01) was significant in comparison with negative control group. Delay in tonic-colonic seizures in dose of 1000 mg/kg (p<0.001) and doses of 300, 500 and 800 mg/kg (p<0.05) was significant in comparison with negative control group. CONCLUSION: Regarding to our findings, it seems that extract of this herb has decremental effect on PTZ-induced seizure in male adult mice.F Keihanian,M Rostampour VajariAA Saeidynia,AR ElmiehBabol University of Medical SciencesarticlerueseizurepentylenetetrazolemiceMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 4, Pp 30-36 (2012)
institution DOAJ
collection DOAJ
language EN
FA
topic rue
seizure
pentylenetetrazole
mice
Medicine
R
Medicine (General)
R5-920
spellingShingle rue
seizure
pentylenetetrazole
mice
Medicine
R
Medicine (General)
R5-920
F Keihanian,
M Rostampour VajariA
A Saeidynia,
AR Elmieh
Effect of Ruta Graveolens Hydro-Alcoholic Extract on Pentylenetetrazole-Induced Seizure in Male Mice
description BACKGROUND AND OBJECTIVE: Seizure is an important symptom of epilepsy and many other neurogenic disorders. Despite a variety of current anti-convulsive drugs, research for discovering new drugs with more efficacy and less unsuitable adverse effects has been continued. Herbal medicine has various natural substances and proper context for this type of research. The aim of this study was to survey the anticonvulsant effects of hydro-alcoholic extract of rue (Ruta graveolens) in male mice.METHODS: In this experimental study anticonvulsant effect of extract evaluated by PTZ induced seizure. Fifty-six NMRI mice in the range of 25-35 gr were divided to 7 groups of 8 mice that included five cases, one positive control group and one negative control group. Case groups have been injected by 100, 300, 500, 800 and 1000 mg/kg of extracts, positive control group 40 ml/kg phenobarbital and negative control group 10 ml/kg normal saline intraperitoneally. All groups were injected by pentylenetetrazole (80mg/kg) intraperitoneally after 45 minutes, and initiation time of myocolonic and tonic-colonic seizures and rate of 24 hours death were measured.FINDINGS: Consequences of different doses of rue hydro-alcoholic extract increased delay in initiation of myocolonic and tonic-colonic seizures rather than control group dose-dependently and reduced 24 hours seizure-induced mortality (p<0.05). Delay in myocolonic seizures in doses of 300, 500, 800 and 1000 mg/kg (p<0.001) and 100 mg/kg (p<0.01) was significant in comparison with negative control group. Delay in tonic-colonic seizures in dose of 1000 mg/kg (p<0.001) and doses of 300, 500 and 800 mg/kg (p<0.05) was significant in comparison with negative control group. CONCLUSION: Regarding to our findings, it seems that extract of this herb has decremental effect on PTZ-induced seizure in male adult mice.
format article
author F Keihanian,
M Rostampour VajariA
A Saeidynia,
AR Elmieh
author_facet F Keihanian,
M Rostampour VajariA
A Saeidynia,
AR Elmieh
author_sort F Keihanian,
title Effect of Ruta Graveolens Hydro-Alcoholic Extract on Pentylenetetrazole-Induced Seizure in Male Mice
title_short Effect of Ruta Graveolens Hydro-Alcoholic Extract on Pentylenetetrazole-Induced Seizure in Male Mice
title_full Effect of Ruta Graveolens Hydro-Alcoholic Extract on Pentylenetetrazole-Induced Seizure in Male Mice
title_fullStr Effect of Ruta Graveolens Hydro-Alcoholic Extract on Pentylenetetrazole-Induced Seizure in Male Mice
title_full_unstemmed Effect of Ruta Graveolens Hydro-Alcoholic Extract on Pentylenetetrazole-Induced Seizure in Male Mice
title_sort effect of ruta graveolens hydro-alcoholic extract on pentylenetetrazole-induced seizure in male mice
publisher Babol University of Medical Sciences
publishDate 2012
url https://doaj.org/article/7528386db2074c64b439ec0fedb565bc
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AT mrostampourvajaria effectofrutagraveolenshydroalcoholicextractonpentylenetetrazoleinducedseizureinmalemice
AT asaeidynia effectofrutagraveolenshydroalcoholicextractonpentylenetetrazoleinducedseizureinmalemice
AT arelmieh effectofrutagraveolenshydroalcoholicextractonpentylenetetrazoleinducedseizureinmalemice
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