Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells

In consideration of improving the interface problems of poly-L-lactic acid (PLLA) that hindered biomedical use, surface coatings have been explored as an appealing strategy in establishing a multi-functional coating for osteogenesis. Though the layer-by-layer (LBL) coating developed, a few studies h...

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Autores principales:	Yiqun Ma, Yuwang You, Lu Cao, Bing Liang, Bo Tian, Jian Dong, Hong Lin
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	hydrogel coating gelatin methacrylate (GelMA) alginate double crosslink mesenchymal stem cells Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/753b978d9bcd4e189aca80574201b602
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spelling	oai:doaj.org-article:753b978d9bcd4e189aca80574201b6022021-12-01T19:14:37ZImproved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells2296-418510.3389/fbioe.2021.706423https://doaj.org/article/753b978d9bcd4e189aca80574201b6022021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.706423/fullhttps://doaj.org/toc/2296-4185In consideration of improving the interface problems of poly-L-lactic acid (PLLA) that hindered biomedical use, surface coatings have been explored as an appealing strategy in establishing a multi-functional coating for osteogenesis. Though the layer-by-layer (LBL) coating developed, a few studies have applied double-crosslinked hydrogels in this technique. In this research, we established a bilayer coating with double-crosslinked hydrogels [alginate–gelatin methacrylate (GelMA)] containing bone morphogenic protein (BMP)-2 [alginate-GelMA/hydroxyapatite (HA)/BMP-2], which displayed great biocompatibility and osteogenesis. The characterization of the coating showed improved properties and enhanced wettability of the native PLLA. To evaluate the biosafety and inductive ability of osteogenesis, the behavior (viability, adherence, and proliferation) and morphology of human bone mesenchymal stem cells (hBMSCs) on the bilayer coatings were tested by multiple exams. The satisfactory function of osteogenesis was verified in bilayer coatings. We found the best ratios between GelMA and alginate for biological applications. The Alg70-Gel30 and Alg50-Gel50 groups facilitated the osteogenic transformation of hBMSCs. In brief, alginate-GelMA/HA/BMP-2 could increase the hBMSCs’ early transformation of osteoblast lineage and promote the osteogenesis of bone defect, especially the outer hydrogel layer such as Alg70-Gel30 and Alg50-Gel50.Yiqun MaYuwang YouLu CaoBing LiangBo TianJian DongHong LinFrontiers Media S.A.articlehydrogel coatinggelatin methacrylate (GelMA)alginatedouble crosslinkmesenchymal stem cellsBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrogel coating gelatin methacrylate (GelMA) alginate double crosslink mesenchymal stem cells Biotechnology TP248.13-248.65
spellingShingle	hydrogel coating gelatin methacrylate (GelMA) alginate double crosslink mesenchymal stem cells Biotechnology TP248.13-248.65 Yiqun Ma Yuwang You Lu Cao Bing Liang Bo Tian Jian Dong Hong Lin Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells
description	In consideration of improving the interface problems of poly-L-lactic acid (PLLA) that hindered biomedical use, surface coatings have been explored as an appealing strategy in establishing a multi-functional coating for osteogenesis. Though the layer-by-layer (LBL) coating developed, a few studies have applied double-crosslinked hydrogels in this technique. In this research, we established a bilayer coating with double-crosslinked hydrogels [alginate–gelatin methacrylate (GelMA)] containing bone morphogenic protein (BMP)-2 [alginate-GelMA/hydroxyapatite (HA)/BMP-2], which displayed great biocompatibility and osteogenesis. The characterization of the coating showed improved properties and enhanced wettability of the native PLLA. To evaluate the biosafety and inductive ability of osteogenesis, the behavior (viability, adherence, and proliferation) and morphology of human bone mesenchymal stem cells (hBMSCs) on the bilayer coatings were tested by multiple exams. The satisfactory function of osteogenesis was verified in bilayer coatings. We found the best ratios between GelMA and alginate for biological applications. The Alg70-Gel30 and Alg50-Gel50 groups facilitated the osteogenic transformation of hBMSCs. In brief, alginate-GelMA/HA/BMP-2 could increase the hBMSCs’ early transformation of osteoblast lineage and promote the osteogenesis of bone defect, especially the outer hydrogel layer such as Alg70-Gel30 and Alg50-Gel50.
format	article
author	Yiqun Ma Yuwang You Lu Cao Bing Liang Bo Tian Jian Dong Hong Lin
author_facet	Yiqun Ma Yuwang You Lu Cao Bing Liang Bo Tian Jian Dong Hong Lin
author_sort	Yiqun Ma
title	Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells
title_short	Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells
title_full	Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells
title_fullStr	Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells
title_full_unstemmed	Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells
title_sort	improved osteogenesis by mineralization combined with double-crosslinked hydrogel coating for proliferation and differentiation of mesenchymal stem cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/753b978d9bcd4e189aca80574201b602
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Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells

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