Production of human CAR-NK cells with lentiviral vectors and functional assessment in vitro
Summary: Although natural killer (NK) cells have become a promising immune effector cell for chimeric antigen receptor (CAR)-based therapy, generating human CAR-NK cells with high transgene efficiency has been challenging. In this protocol, we describe how to generate CAR-NK cells with transduction...
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Main Authors: | Ana L. Portillo, Richard Hogg, Ali A. Ashkar |
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Format: | article |
Language: | EN |
Published: |
Elsevier
2021
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Subjects: | |
Online Access: | https://doaj.org/article/7540abfaed4544eaa9ea2867d0c58adb |
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