The Effects of Solvent Fractions of Bee Pollen Crude Extract in an Animal Model of Inflammation

BACKGROUND AND OBJECTIVE: Conventional anti-inflammatory drugs, despite their beneficial effects, have serious and irreversible side effects. Due to the remarkable anti-inflammatory properties of bee pollen crude extract, the present study was conducted to investigate the effects of 100% methanol fr...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: T Eteraf-Oskouei, F Javanmard, A Moghadami-kia, M Najafi
Formato: article
Lenguaje:EN
FA
Publicado: Babol University of Medical Sciences 2021
Materias:
R
Acceso en línea:https://doaj.org/article/7543820806534cb993e2701854f400a4
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:7543820806534cb993e2701854f400a4
record_format dspace
spelling oai:doaj.org-article:7543820806534cb993e2701854f400a42021-11-29T07:40:49ZThe Effects of Solvent Fractions of Bee Pollen Crude Extract in an Animal Model of Inflammation1561-41072251-7170https://doaj.org/article/7543820806534cb993e2701854f400a42021-03-01T00:00:00Zhttp://jbums.org/article-1-9863-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Conventional anti-inflammatory drugs, despite their beneficial effects, have serious and irreversible side effects. Due to the remarkable anti-inflammatory properties of bee pollen crude extract, the present study was conducted to investigate the effects of 100% methanol fraction (MF) and dichloromethane fraction (DF) in an air pouch model of inflammation. METHODS: This experimental study was performed on 42 male Wistar rats in 7 groups of six. First, the hairs on the back of the rats were shaved. Subcutaneous injection of sterile air (20 and 10 mL) was performed on the dorsum of anaesthetized rats (day 1 and day 3, respectively). On day 6, inflammation was induced by intra-pouch injection of carrageenan. Group I: Normal saline; Group II, III, and IV: the rats treated with 100, 500 and 2500 μg MF; Group V, VI, and VII: the animals treated with 100, 500 and 2500 μg DF by intra-pouch injection simultaneously with carrageenan after 24 and 48 h. Three days after carrageenan administration, the inflammatory parameters and angiogenesis were measured. FINDINGS: Compared to the control group (5.3±0.5 g), granulation tissue weight was significantly decreased (p<0.001) by approximately 30% and 40% by all three doses of MF and DF, respectively. Inflammatory parameters such as exudate volume and angiogenesis were significantly reduced by DF (p<0.001) and MF (p<0.01), respectively. In both fractions, a dose of 2500 μg increased the number of leukocytes. CONCLUSION: Although different effects were observed in the two fractions of extract due to different amounts of flavonoids, a promising inhibitory effect on granulation tissue and angiogenesis was obtained, which could be considered as a natural source of anti-rheumatoid and anti-cancer agents.T Eteraf-OskoueiF JavanmardA Moghadami-kiaM NajafiBabol University of Medical Sciencesarticleapitherapyair pouchinflammationangiogenesis inhibitors.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 23, Iss 1, Pp 297-303 (2021)
institution DOAJ
collection DOAJ
language EN
FA
topic apitherapy
air pouch
inflammation
angiogenesis inhibitors.
Medicine
R
Medicine (General)
R5-920
spellingShingle apitherapy
air pouch
inflammation
angiogenesis inhibitors.
Medicine
R
Medicine (General)
R5-920
T Eteraf-Oskouei
F Javanmard
A Moghadami-kia
M Najafi
The Effects of Solvent Fractions of Bee Pollen Crude Extract in an Animal Model of Inflammation
description BACKGROUND AND OBJECTIVE: Conventional anti-inflammatory drugs, despite their beneficial effects, have serious and irreversible side effects. Due to the remarkable anti-inflammatory properties of bee pollen crude extract, the present study was conducted to investigate the effects of 100% methanol fraction (MF) and dichloromethane fraction (DF) in an air pouch model of inflammation. METHODS: This experimental study was performed on 42 male Wistar rats in 7 groups of six. First, the hairs on the back of the rats were shaved. Subcutaneous injection of sterile air (20 and 10 mL) was performed on the dorsum of anaesthetized rats (day 1 and day 3, respectively). On day 6, inflammation was induced by intra-pouch injection of carrageenan. Group I: Normal saline; Group II, III, and IV: the rats treated with 100, 500 and 2500 μg MF; Group V, VI, and VII: the animals treated with 100, 500 and 2500 μg DF by intra-pouch injection simultaneously with carrageenan after 24 and 48 h. Three days after carrageenan administration, the inflammatory parameters and angiogenesis were measured. FINDINGS: Compared to the control group (5.3±0.5 g), granulation tissue weight was significantly decreased (p<0.001) by approximately 30% and 40% by all three doses of MF and DF, respectively. Inflammatory parameters such as exudate volume and angiogenesis were significantly reduced by DF (p<0.001) and MF (p<0.01), respectively. In both fractions, a dose of 2500 μg increased the number of leukocytes. CONCLUSION: Although different effects were observed in the two fractions of extract due to different amounts of flavonoids, a promising inhibitory effect on granulation tissue and angiogenesis was obtained, which could be considered as a natural source of anti-rheumatoid and anti-cancer agents.
format article
author T Eteraf-Oskouei
F Javanmard
A Moghadami-kia
M Najafi
author_facet T Eteraf-Oskouei
F Javanmard
A Moghadami-kia
M Najafi
author_sort T Eteraf-Oskouei
title The Effects of Solvent Fractions of Bee Pollen Crude Extract in an Animal Model of Inflammation
title_short The Effects of Solvent Fractions of Bee Pollen Crude Extract in an Animal Model of Inflammation
title_full The Effects of Solvent Fractions of Bee Pollen Crude Extract in an Animal Model of Inflammation
title_fullStr The Effects of Solvent Fractions of Bee Pollen Crude Extract in an Animal Model of Inflammation
title_full_unstemmed The Effects of Solvent Fractions of Bee Pollen Crude Extract in an Animal Model of Inflammation
title_sort effects of solvent fractions of bee pollen crude extract in an animal model of inflammation
publisher Babol University of Medical Sciences
publishDate 2021
url https://doaj.org/article/7543820806534cb993e2701854f400a4
work_keys_str_mv AT teterafoskouei theeffectsofsolventfractionsofbeepollencrudeextractinananimalmodelofinflammation
AT fjavanmard theeffectsofsolventfractionsofbeepollencrudeextractinananimalmodelofinflammation
AT amoghadamikia theeffectsofsolventfractionsofbeepollencrudeextractinananimalmodelofinflammation
AT mnajafi theeffectsofsolventfractionsofbeepollencrudeextractinananimalmodelofinflammation
AT teterafoskouei effectsofsolventfractionsofbeepollencrudeextractinananimalmodelofinflammation
AT fjavanmard effectsofsolventfractionsofbeepollencrudeextractinananimalmodelofinflammation
AT amoghadamikia effectsofsolventfractionsofbeepollencrudeextractinananimalmodelofinflammation
AT mnajafi effectsofsolventfractionsofbeepollencrudeextractinananimalmodelofinflammation
_version_ 1718407532624478208