Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds

Abstract Cerebrospinal fluid (CSF) proteins regulate neurogenesis, brain homeostasis and participate in signalling during neuroinflammation. Even though birds represent valuable models for constitutive adult neurogenesis, current proteomic studies of the avian CSF are limited to chicken embryos. Her...

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Autores principales:	Eleni Voukali, Nithya Kuttiyarthu Veetil, Pavel Němec, Pavel Stopka, Michal Vinkler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/754b3ed5515e4fb2a0a02f599d8534d9
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spelling	oai:doaj.org-article:754b3ed5515e4fb2a0a02f599d8534d92021-12-02T15:54:13ZComparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds10.1038/s41598-021-84274-x2045-2322https://doaj.org/article/754b3ed5515e4fb2a0a02f599d8534d92021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84274-xhttps://doaj.org/toc/2045-2322Abstract Cerebrospinal fluid (CSF) proteins regulate neurogenesis, brain homeostasis and participate in signalling during neuroinflammation. Even though birds represent valuable models for constitutive adult neurogenesis, current proteomic studies of the avian CSF are limited to chicken embryos. Here we use liquid chromatography–tandem mass spectrometry (nLC-MS/MS) to explore the proteomic composition of CSF and plasma in adult chickens (Gallus gallus) and evolutionarily derived parrots: budgerigar (Melopsittacus undulatus) and cockatiel (Nymphicus hollandicus). Because cockatiel lacks a complete genome information, we compared the cross-species protein identifications using the reference proteomes of three model avian species: chicken, budgerigar and zebra finch (Taeniopygia guttata) and found the highest identification rates when mapping against the phylogenetically closest species, the budgerigar. In total, we identified 483, 641 and 458 unique proteins consistently represented in the CSF and plasma of all chicken, budgerigar and cockatiel conspecifics, respectively. Comparative pathways analyses of CSF and blood plasma then indicated clusters of proteins involved in neurogenesis, neural development and neural differentiation overrepresented in CSF in each species. This study provides the first insight into the proteomics of adult avian CSF and plasma and brings novel evidence supporting the adult neurogenesis in birds.Eleni VoukaliNithya Kuttiyarthu VeetilPavel NěmecPavel StopkaMichal VinklerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eleni Voukali Nithya Kuttiyarthu Veetil Pavel Němec Pavel Stopka Michal Vinkler Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds
description	Abstract Cerebrospinal fluid (CSF) proteins regulate neurogenesis, brain homeostasis and participate in signalling during neuroinflammation. Even though birds represent valuable models for constitutive adult neurogenesis, current proteomic studies of the avian CSF are limited to chicken embryos. Here we use liquid chromatography–tandem mass spectrometry (nLC-MS/MS) to explore the proteomic composition of CSF and plasma in adult chickens (Gallus gallus) and evolutionarily derived parrots: budgerigar (Melopsittacus undulatus) and cockatiel (Nymphicus hollandicus). Because cockatiel lacks a complete genome information, we compared the cross-species protein identifications using the reference proteomes of three model avian species: chicken, budgerigar and zebra finch (Taeniopygia guttata) and found the highest identification rates when mapping against the phylogenetically closest species, the budgerigar. In total, we identified 483, 641 and 458 unique proteins consistently represented in the CSF and plasma of all chicken, budgerigar and cockatiel conspecifics, respectively. Comparative pathways analyses of CSF and blood plasma then indicated clusters of proteins involved in neurogenesis, neural development and neural differentiation overrepresented in CSF in each species. This study provides the first insight into the proteomics of adult avian CSF and plasma and brings novel evidence supporting the adult neurogenesis in birds.
format	article
author	Eleni Voukali Nithya Kuttiyarthu Veetil Pavel Němec Pavel Stopka Michal Vinkler
author_facet	Eleni Voukali Nithya Kuttiyarthu Veetil Pavel Němec Pavel Stopka Michal Vinkler
author_sort	Eleni Voukali
title	Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds
title_short	Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds
title_full	Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds
title_fullStr	Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds
title_full_unstemmed	Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds
title_sort	comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/754b3ed5515e4fb2a0a02f599d8534d9
work_keys_str_mv	AT elenivoukali comparisonofplasmaandcerebrospinalfluidproteomesidentifiesgeneproductsguidingadultneurogenesisandneuraldifferentiationinbirds AT nithyakuttiyarthuveetil comparisonofplasmaandcerebrospinalfluidproteomesidentifiesgeneproductsguidingadultneurogenesisandneuraldifferentiationinbirds AT pavelnemec comparisonofplasmaandcerebrospinalfluidproteomesidentifiesgeneproductsguidingadultneurogenesisandneuraldifferentiationinbirds AT pavelstopka comparisonofplasmaandcerebrospinalfluidproteomesidentifiesgeneproductsguidingadultneurogenesisandneuraldifferentiationinbirds AT michalvinkler comparisonofplasmaandcerebrospinalfluidproteomesidentifiesgeneproductsguidingadultneurogenesisandneuraldifferentiationinbirds
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Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds

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