Increased abscess formation and defective chemokine regulation in CREB transgenic mice.

Cyclic AMP-response element-binding protein (CREB) is a transcription factor implicated in growth factor-dependent cell proliferation and survival, glucose homeostasis, spermatogenesis, circadian rhythms, and synaptic plasticity associated with memory. To study the phenotype of CREB overexpression i...

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Autores principales:	Andy Y Wen, Elliot M Landaw, Rachel Ochoa, Michelle Cho, Alex Chao, Gregory Lawson, Kathleen M Sakamoto
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/75548741c0c64e87a82a55f0799038bf
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spelling	oai:doaj.org-article:75548741c0c64e87a82a55f0799038bf2021-11-18T07:58:30ZIncreased abscess formation and defective chemokine regulation in CREB transgenic mice.1932-620310.1371/journal.pone.0055866https://doaj.org/article/75548741c0c64e87a82a55f0799038bf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23405224/?tool=EBIhttps://doaj.org/toc/1932-6203Cyclic AMP-response element-binding protein (CREB) is a transcription factor implicated in growth factor-dependent cell proliferation and survival, glucose homeostasis, spermatogenesis, circadian rhythms, and synaptic plasticity associated with memory. To study the phenotype of CREB overexpression in vivo, we generated CREB transgenic (TG) mice in which a myeloid specific hMRP8 promoter drives CREB expression. CREB TG mice developed spontaneous skin abscesses more frequently than wild type (WT) mice. To understand the role of CREB in myeloid function and innate immunity, chemokine expression in bone marrow derived macrophages (BMDMs) from CREB TG mice were compared with BMDMs from WT mice. Our results demonstrated decreased Keratinocyte-derived cytokine (KC) in CREB TG BMDMs but not TNFα protein production in response to lipid A (LPA). In addition, mRNA expression of KC and IL-1β (Interleukin)-1β was decreased in CREB TG BMDMs; however, there was no difference in the mRNA expression of TNFα, MCP-1, IL-6 and IL-12p40. The mRNA expression of IL-1RA and IL-10 was decreased in response to LPA. Nuclear factor kappa B (NFκB) expression and a subset of its target genes were upregulated in CREB TG mouse BMDMs. Although neutrophil migration was the same in both CREB TG and WT mice, Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity was significantly increased in neutrophils from CREB TG mice. Taken together, CREB overexpression in myeloid cells results in increased abscess formation in vivo and aberrant cytokine and chemokine response, and neutrophil function in vitro.Andy Y WenElliot M LandawRachel OchoaMichelle ChoAlex ChaoGregory LawsonKathleen M SakamotoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55866 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andy Y Wen Elliot M Landaw Rachel Ochoa Michelle Cho Alex Chao Gregory Lawson Kathleen M Sakamoto Increased abscess formation and defective chemokine regulation in CREB transgenic mice.
description	Cyclic AMP-response element-binding protein (CREB) is a transcription factor implicated in growth factor-dependent cell proliferation and survival, glucose homeostasis, spermatogenesis, circadian rhythms, and synaptic plasticity associated with memory. To study the phenotype of CREB overexpression in vivo, we generated CREB transgenic (TG) mice in which a myeloid specific hMRP8 promoter drives CREB expression. CREB TG mice developed spontaneous skin abscesses more frequently than wild type (WT) mice. To understand the role of CREB in myeloid function and innate immunity, chemokine expression in bone marrow derived macrophages (BMDMs) from CREB TG mice were compared with BMDMs from WT mice. Our results demonstrated decreased Keratinocyte-derived cytokine (KC) in CREB TG BMDMs but not TNFα protein production in response to lipid A (LPA). In addition, mRNA expression of KC and IL-1β (Interleukin)-1β was decreased in CREB TG BMDMs; however, there was no difference in the mRNA expression of TNFα, MCP-1, IL-6 and IL-12p40. The mRNA expression of IL-1RA and IL-10 was decreased in response to LPA. Nuclear factor kappa B (NFκB) expression and a subset of its target genes were upregulated in CREB TG mouse BMDMs. Although neutrophil migration was the same in both CREB TG and WT mice, Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity was significantly increased in neutrophils from CREB TG mice. Taken together, CREB overexpression in myeloid cells results in increased abscess formation in vivo and aberrant cytokine and chemokine response, and neutrophil function in vitro.
format	article
author	Andy Y Wen Elliot M Landaw Rachel Ochoa Michelle Cho Alex Chao Gregory Lawson Kathleen M Sakamoto
author_facet	Andy Y Wen Elliot M Landaw Rachel Ochoa Michelle Cho Alex Chao Gregory Lawson Kathleen M Sakamoto
author_sort	Andy Y Wen
title	Increased abscess formation and defective chemokine regulation in CREB transgenic mice.
title_short	Increased abscess formation and defective chemokine regulation in CREB transgenic mice.
title_full	Increased abscess formation and defective chemokine regulation in CREB transgenic mice.
title_fullStr	Increased abscess formation and defective chemokine regulation in CREB transgenic mice.
title_full_unstemmed	Increased abscess formation and defective chemokine regulation in CREB transgenic mice.
title_sort	increased abscess formation and defective chemokine regulation in creb transgenic mice.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/75548741c0c64e87a82a55f0799038bf
work_keys_str_mv	AT andyywen increasedabscessformationanddefectivechemokineregulationincrebtransgenicmice AT elliotmlandaw increasedabscessformationanddefectivechemokineregulationincrebtransgenicmice AT rachelochoa increasedabscessformationanddefectivechemokineregulationincrebtransgenicmice AT michellecho increasedabscessformationanddefectivechemokineregulationincrebtransgenicmice AT alexchao increasedabscessformationanddefectivechemokineregulationincrebtransgenicmice AT gregorylawson increasedabscessformationanddefectivechemokineregulationincrebtransgenicmice AT kathleenmsakamoto increasedabscessformationanddefectivechemokineregulationincrebtransgenicmice
_version_	1718422674501271552

Increased abscess formation and defective chemokine regulation in CREB transgenic mice.

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