Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells
Oxidative stress plays a crucial role in the development of airway diseases. Recently, hydrogen (H<sub>2</sub>) gas has been explored for its antioxidant properties. This study investigated the role of H<sub>2</sub> gas in oxidative stress-induced alveolar and bronchial airwa...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | article |
| Language: | EN |
| Published: |
MDPI AG
2021
|
| Subjects: | |
| Online Access: | https://doaj.org/article/755c313f7baf46278d73ddb805aa1958 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Oxidative stress plays a crucial role in the development of airway diseases. Recently, hydrogen (H<sub>2</sub>) gas has been explored for its antioxidant properties. This study investigated the role of H<sub>2</sub> gas in oxidative stress-induced alveolar and bronchial airway injury, where A549 and NCI-H292 cells were stimulated with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and lipopolysaccharide (LPS) in vitro. Results show that time-dependent administration of 2% H<sub>2</sub> gas recovered the cells from oxidative stress. Various indicators including reactive oxygen species (ROS), nitric oxide (NO), antioxidant enzymes (catalase, glutathione peroxidase), intracellular calcium, and mitogen-activated protein kinase (MAPK) signaling pathway were examined to analyze the redox profile. The viability of A549 and NCI-H292 cells and the activity of antioxidant enzymes were reduced following induction by H<sub>2</sub>O<sub>2</sub> and LPS but were later recovered using H<sub>2</sub> gas. Additionally, the levels of oxidative stress markers, including ROS and NO, were elevated upon induction but were attenuated after treatment with H<sub>2</sub> gas. Furthermore, H<sub>2</sub> gas suppressed oxidative stress-induced MAPK activation and maintained calcium homeostasis. This study suggests that H<sub>2</sub> gas can rescue airway epithelial cells from H<sub>2</sub>O<sub>2</sub> and LPS-induced oxidative stress and may be a potential intervention for airway diseases. |
|---|