Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells

Oxidative stress plays a crucial role in the development of airway diseases. Recently, hydrogen (H<sub>2</sub>) gas has been explored for its antioxidant properties. This study investigated the role of H<sub>2</sub> gas in oxidative stress-induced alveolar and bronchial airwa...

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Autores principales:	In-Soo You, Subham Sharma, Ailyn Fadriquela, Johny Bajgai, Thuy Trinh Thi, Md. Habibur Rahman, Jaeyong Sung, Hwang-Un Kwon, So-Yeon Lee, Cheol-Su Kim, Kyu-Jae Lee
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hydrogen gas oxidative stress anti-oxidant airway epithelium MAPK signaling Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/755c313f7baf46278d73ddb805aa1958
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spelling	oai:doaj.org-article:755c313f7baf46278d73ddb805aa19582021-11-11T18:24:17ZAntioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells10.3390/molecules262163751420-3049https://doaj.org/article/755c313f7baf46278d73ddb805aa19582021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6375https://doaj.org/toc/1420-3049Oxidative stress plays a crucial role in the development of airway diseases. Recently, hydrogen (H<sub>2</sub>) gas has been explored for its antioxidant properties. This study investigated the role of H<sub>2</sub> gas in oxidative stress-induced alveolar and bronchial airway injury, where A549 and NCI-H292 cells were stimulated with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and lipopolysaccharide (LPS) in vitro. Results show that time-dependent administration of 2% H<sub>2</sub> gas recovered the cells from oxidative stress. Various indicators including reactive oxygen species (ROS), nitric oxide (NO), antioxidant enzymes (catalase, glutathione peroxidase), intracellular calcium, and mitogen-activated protein kinase (MAPK) signaling pathway were examined to analyze the redox profile. The viability of A549 and NCI-H292 cells and the activity of antioxidant enzymes were reduced following induction by H<sub>2</sub>O<sub>2</sub> and LPS but were later recovered using H<sub>2</sub> gas. Additionally, the levels of oxidative stress markers, including ROS and NO, were elevated upon induction but were attenuated after treatment with H<sub>2</sub> gas. Furthermore, H<sub>2</sub> gas suppressed oxidative stress-induced MAPK activation and maintained calcium homeostasis. This study suggests that H<sub>2</sub> gas can rescue airway epithelial cells from H<sub>2</sub>O<sub>2</sub> and LPS-induced oxidative stress and may be a potential intervention for airway diseases.In-Soo YouSubham SharmaAilyn FadriquelaJohny BajgaiThuy Trinh ThiMd. Habibur RahmanJaeyong SungHwang-Un KwonSo-Yeon LeeCheol-Su KimKyu-Jae LeeMDPI AGarticlehydrogen gasoxidative stressanti-oxidantairway epitheliumMAPK signalingOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6375, p 6375 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrogen gas oxidative stress anti-oxidant airway epithelium MAPK signaling Organic chemistry QD241-441
spellingShingle	hydrogen gas oxidative stress anti-oxidant airway epithelium MAPK signaling Organic chemistry QD241-441 In-Soo You Subham Sharma Ailyn Fadriquela Johny Bajgai Thuy Trinh Thi Md. Habibur Rahman Jaeyong Sung Hwang-Un Kwon So-Yeon Lee Cheol-Su Kim Kyu-Jae Lee Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells
description	Oxidative stress plays a crucial role in the development of airway diseases. Recently, hydrogen (H<sub>2</sub>) gas has been explored for its antioxidant properties. This study investigated the role of H<sub>2</sub> gas in oxidative stress-induced alveolar and bronchial airway injury, where A549 and NCI-H292 cells were stimulated with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and lipopolysaccharide (LPS) in vitro. Results show that time-dependent administration of 2% H<sub>2</sub> gas recovered the cells from oxidative stress. Various indicators including reactive oxygen species (ROS), nitric oxide (NO), antioxidant enzymes (catalase, glutathione peroxidase), intracellular calcium, and mitogen-activated protein kinase (MAPK) signaling pathway were examined to analyze the redox profile. The viability of A549 and NCI-H292 cells and the activity of antioxidant enzymes were reduced following induction by H<sub>2</sub>O<sub>2</sub> and LPS but were later recovered using H<sub>2</sub> gas. Additionally, the levels of oxidative stress markers, including ROS and NO, were elevated upon induction but were attenuated after treatment with H<sub>2</sub> gas. Furthermore, H<sub>2</sub> gas suppressed oxidative stress-induced MAPK activation and maintained calcium homeostasis. This study suggests that H<sub>2</sub> gas can rescue airway epithelial cells from H<sub>2</sub>O<sub>2</sub> and LPS-induced oxidative stress and may be a potential intervention for airway diseases.
format	article
author	In-Soo You Subham Sharma Ailyn Fadriquela Johny Bajgai Thuy Trinh Thi Md. Habibur Rahman Jaeyong Sung Hwang-Un Kwon So-Yeon Lee Cheol-Su Kim Kyu-Jae Lee
author_facet	In-Soo You Subham Sharma Ailyn Fadriquela Johny Bajgai Thuy Trinh Thi Md. Habibur Rahman Jaeyong Sung Hwang-Un Kwon So-Yeon Lee Cheol-Su Kim Kyu-Jae Lee
author_sort	In-Soo You
title	Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells
title_short	Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells
title_full	Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells
title_fullStr	Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells
title_full_unstemmed	Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells
title_sort	antioxidant properties of hydrogen gas attenuates oxidative stress in airway epithelial cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/755c313f7baf46278d73ddb805aa1958
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Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells

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