Reproducibility of nasal allergen challenge responses in adults with allergic rhinitis

Charles T Pantin,1 Thomas Southworth,1 Kristiane Wetzel,2 Dave Singh1 1Medicines Evaluation Unit, Manchester Academic Health Science Centre, Manchester University NHS Foundation Trust, University of Manchester, Manchester, UK; 2Translational Medicine and Clinical Pharmacology, Boehringer Ingelheim P...

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Autores principales:	Pantin CT, Southworth T, Wetzel K, Singh D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Allergic rhinitis Nasal allergen challenge Polyvinyl acetate sponges IL-5 CXCL8 CCL11 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/7563137f17b84e78bd0757d64d18ac1b
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spelling	oai:doaj.org-article:7563137f17b84e78bd0757d64d18ac1b2021-12-02T06:56:21ZReproducibility of nasal allergen challenge responses in adults with allergic rhinitis1179-1438https://doaj.org/article/7563137f17b84e78bd0757d64d18ac1b2019-05-01T00:00:00Zhttps://www.dovepress.com/reproducibility-of-nasal-allergen-challenge-responses-in-adults-with-a-peer-reviewed-article-CPAAhttps://doaj.org/toc/1179-1438Charles T Pantin,1 Thomas Southworth,1 Kristiane Wetzel,2 Dave Singh1 1Medicines Evaluation Unit, Manchester Academic Health Science Centre, Manchester University NHS Foundation Trust, University of Manchester, Manchester, UK; 2Translational Medicine and Clinical Pharmacology, Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riss, Germany Background: Allergic rhinitis is characterized by nasal inflammation in response to allergen exposure. Nasal allergen challenges are used in clinical trials evaluating drug effects. Reproducibility of nasal secretion cytokine responses and physiological measurements are needed to determine the optimum measurements and power calculations for future studies. We have investigated the reproducibility of nasal cytokine measurements, using ready-to-use polyvinyl acetate sponges to collect nasal secretions, and measurements of nasal physiological responses.Methods: Twelve subjects with allergic rhinitis and no history of respiratory disease, and 12 subjects with asthma and allergic rhinitis underwent a nasal allergen challenge. This was repeated at 7–14 days later.Results: There were increases in IL-5, CCL11, and CXCL8 responses post-challenge (all P<0.05). There was better reproducibility at later time points when higher cytokine levels were detected for IL-5 (ri =0.64 at 8 hours) and CXCL8 (ri =0.91 at 8 hours). Acoustic rhinometry provided good to excellent reproducibility (ri =0.66–0.89). Rhinomanometry had lower reproducibility with greater variation (ri =0.10–0.70), with some subjects unable to perform the measurement. Multiplex immunoassays provided greater sensitivity for CCL11 measurements. There were no differences between allergic rhinitis patients with and without asthma.Conclusion: Polyvinyl acetate sponges are a practical and reproducible way to sample nasal secretions. Acoustic rhinometry is a practical and reproducible method for assessing physiological responses. There were no differences in nasal response due to the presence of concurrent asthma. Keywords: acoustic rhinometry, total nasal symptom score, polyvinyl acetate sponges, IL-5, CXCL8, CCL11Pantin CTSouthworth TWetzel KSingh DDove Medical PressarticleAllergic rhinitisNasal allergen challengePolyvinyl acetate spongesIL-5CXCL8CCL11Therapeutics. PharmacologyRM1-950ENClinical Pharmacology: Advances and Applications, Vol Volume 11, Pp 67-76 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Allergic rhinitis Nasal allergen challenge Polyvinyl acetate sponges IL-5 CXCL8 CCL11 Therapeutics. Pharmacology RM1-950
spellingShingle	Allergic rhinitis Nasal allergen challenge Polyvinyl acetate sponges IL-5 CXCL8 CCL11 Therapeutics. Pharmacology RM1-950 Pantin CT Southworth T Wetzel K Singh D Reproducibility of nasal allergen challenge responses in adults with allergic rhinitis
description	Charles T Pantin,1 Thomas Southworth,1 Kristiane Wetzel,2 Dave Singh1 1Medicines Evaluation Unit, Manchester Academic Health Science Centre, Manchester University NHS Foundation Trust, University of Manchester, Manchester, UK; 2Translational Medicine and Clinical Pharmacology, Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riss, Germany Background: Allergic rhinitis is characterized by nasal inflammation in response to allergen exposure. Nasal allergen challenges are used in clinical trials evaluating drug effects. Reproducibility of nasal secretion cytokine responses and physiological measurements are needed to determine the optimum measurements and power calculations for future studies. We have investigated the reproducibility of nasal cytokine measurements, using ready-to-use polyvinyl acetate sponges to collect nasal secretions, and measurements of nasal physiological responses.Methods: Twelve subjects with allergic rhinitis and no history of respiratory disease, and 12 subjects with asthma and allergic rhinitis underwent a nasal allergen challenge. This was repeated at 7–14 days later.Results: There were increases in IL-5, CCL11, and CXCL8 responses post-challenge (all P<0.05). There was better reproducibility at later time points when higher cytokine levels were detected for IL-5 (ri =0.64 at 8 hours) and CXCL8 (ri =0.91 at 8 hours). Acoustic rhinometry provided good to excellent reproducibility (ri =0.66–0.89). Rhinomanometry had lower reproducibility with greater variation (ri =0.10–0.70), with some subjects unable to perform the measurement. Multiplex immunoassays provided greater sensitivity for CCL11 measurements. There were no differences between allergic rhinitis patients with and without asthma.Conclusion: Polyvinyl acetate sponges are a practical and reproducible way to sample nasal secretions. Acoustic rhinometry is a practical and reproducible method for assessing physiological responses. There were no differences in nasal response due to the presence of concurrent asthma. Keywords: acoustic rhinometry, total nasal symptom score, polyvinyl acetate sponges, IL-5, CXCL8, CCL11
format	article
author	Pantin CT Southworth T Wetzel K Singh D
author_facet	Pantin CT Southworth T Wetzel K Singh D
author_sort	Pantin CT
title	Reproducibility of nasal allergen challenge responses in adults with allergic rhinitis
title_short	Reproducibility of nasal allergen challenge responses in adults with allergic rhinitis
title_full	Reproducibility of nasal allergen challenge responses in adults with allergic rhinitis
title_fullStr	Reproducibility of nasal allergen challenge responses in adults with allergic rhinitis
title_full_unstemmed	Reproducibility of nasal allergen challenge responses in adults with allergic rhinitis
title_sort	reproducibility of nasal allergen challenge responses in adults with allergic rhinitis
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/7563137f17b84e78bd0757d64d18ac1b
work_keys_str_mv	AT pantinct reproducibilityofnasalallergenchallengeresponsesinadultswithallergicrhinitis AT southwortht reproducibilityofnasalallergenchallengeresponsesinadultswithallergicrhinitis AT wetzelk reproducibilityofnasalallergenchallengeresponsesinadultswithallergicrhinitis AT singhd reproducibilityofnasalallergenchallengeresponsesinadultswithallergicrhinitis
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Reproducibility of nasal allergen challenge responses in adults with allergic rhinitis

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