First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs
Plasmid-mediated fosfomycin determinants is a global public health concern due to the increasing dissemination of fosfomycin resistance and limited clinical treatment options. Information about the fosfomycin resistant and molecular genetic among <i>Enterococcus</i> spp. is still lacking...
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2021
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oai:doaj.org-article:756b7b2c26da4b8db3751f54b750baab2021-11-25T17:40:47ZFirst Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs10.3390/genes121116842073-4425https://doaj.org/article/756b7b2c26da4b8db3751f54b750baab2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1684https://doaj.org/toc/2073-4425Plasmid-mediated fosfomycin determinants is a global public health concern due to the increasing dissemination of fosfomycin resistance and limited clinical treatment options. Information about the fosfomycin resistant and molecular genetic among <i>Enterococcus</i> spp. is still lacking. In this study, we found the first plasmid-medieted <i>fosB</i> in <i>Enterococcus faecalis</i> from pigs, and all the fosfomycin resistant <i>Enterococcus</i> spp. (FRE) isolates were multi-drug resistant. S1-PFGE, Southern blot and conjugation experiments indicated that the <i>fosB</i> gene located on ~54.7 kb transferable plasmids<sub>.</sub> Relative competition assay confirmed that the <i>fosB</i><i>-</i>carrying plasmid impaired fitness in recipient <i>E. faecalis</i> JH2-2. Illumina and the MinION sequencing data revealed that both <i>E. faecalis</i> ES-1 and ES-2 isolates belonged to novel ST (ST964), and had 71 SNPs difference. WGS showed that the genetic environments of <i>fosB</i> were diverse among different species, and the linezolid resistance gene <i>optrA</i> was found in the <i>fosB</i>-carrying strains. To summarize, for the first time, we reported plasmid-mediated <i>fosB</i> in <i>E. faecalis</i> from pigs. And, the co-occurrence of <i>fosB</i> and <i>optrA</i> pose a serious threat to public health.Xiaoming WangYi GaoXiao LiuNaiyan SunJinhu HuangLiping WangMDPI AGarticleantimicrobial resistancemolecular geneticfosfomycin resistant<i>Enterococcus</i> spp.GeneticsQH426-470ENGenes, Vol 12, Iss 1684, p 1684 (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
antimicrobial resistance molecular genetic fosfomycin resistant <i>Enterococcus</i> spp. Genetics QH426-470 |
| spellingShingle |
antimicrobial resistance molecular genetic fosfomycin resistant <i>Enterococcus</i> spp. Genetics QH426-470 Xiaoming Wang Yi Gao Xiao Liu Naiyan Sun Jinhu Huang Liping Wang First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| description |
Plasmid-mediated fosfomycin determinants is a global public health concern due to the increasing dissemination of fosfomycin resistance and limited clinical treatment options. Information about the fosfomycin resistant and molecular genetic among <i>Enterococcus</i> spp. is still lacking. In this study, we found the first plasmid-medieted <i>fosB</i> in <i>Enterococcus faecalis</i> from pigs, and all the fosfomycin resistant <i>Enterococcus</i> spp. (FRE) isolates were multi-drug resistant. S1-PFGE, Southern blot and conjugation experiments indicated that the <i>fosB</i> gene located on ~54.7 kb transferable plasmids<sub>.</sub> Relative competition assay confirmed that the <i>fosB</i><i>-</i>carrying plasmid impaired fitness in recipient <i>E. faecalis</i> JH2-2. Illumina and the MinION sequencing data revealed that both <i>E. faecalis</i> ES-1 and ES-2 isolates belonged to novel ST (ST964), and had 71 SNPs difference. WGS showed that the genetic environments of <i>fosB</i> were diverse among different species, and the linezolid resistance gene <i>optrA</i> was found in the <i>fosB</i>-carrying strains. To summarize, for the first time, we reported plasmid-mediated <i>fosB</i> in <i>E. faecalis</i> from pigs. And, the co-occurrence of <i>fosB</i> and <i>optrA</i> pose a serious threat to public health. |
| format |
article |
| author |
Xiaoming Wang Yi Gao Xiao Liu Naiyan Sun Jinhu Huang Liping Wang |
| author_facet |
Xiaoming Wang Yi Gao Xiao Liu Naiyan Sun Jinhu Huang Liping Wang |
| author_sort |
Xiaoming Wang |
| title |
First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| title_short |
First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| title_full |
First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| title_fullStr |
First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| title_full_unstemmed |
First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| title_sort |
first report of the plasmid-mediated <i>fosb</i> gene in <i>enterococcus faecalis</i> from pigs |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/756b7b2c26da4b8db3751f54b750baab |
| work_keys_str_mv |
AT xiaomingwang firstreportoftheplasmidmediatedifosbigeneinienterococcusfaecalisifrompigs AT yigao firstreportoftheplasmidmediatedifosbigeneinienterococcusfaecalisifrompigs AT xiaoliu firstreportoftheplasmidmediatedifosbigeneinienterococcusfaecalisifrompigs AT naiyansun firstreportoftheplasmidmediatedifosbigeneinienterococcusfaecalisifrompigs AT jinhuhuang firstreportoftheplasmidmediatedifosbigeneinienterococcusfaecalisifrompigs AT lipingwang firstreportoftheplasmidmediatedifosbigeneinienterococcusfaecalisifrompigs |
| _version_ |
1718412111605923840 |