Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample

Abstract We recovered the first full-length poxvirus genome, including the terminal hairpin region, directly from complex clinical material using a combination of second generation short read and third generation nanopore sequencing technologies. The complete viral genome sequence was directly recov...

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Autores principales:	Thomas Günther, Ludwig Haas, Malik Alawi, Peter Wohlsein, Jerzy Marks, Adam Grundhoff, Paul Becher, Nicole Fischer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/756dc6fd915c4b93802783d41f08e1ca
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spelling	oai:doaj.org-article:756dc6fd915c4b93802783d41f08e1ca2021-12-02T16:06:50ZRecovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample10.1038/s41598-017-03997-y2045-2322https://doaj.org/article/756dc6fd915c4b93802783d41f08e1ca2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03997-yhttps://doaj.org/toc/2045-2322Abstract We recovered the first full-length poxvirus genome, including the terminal hairpin region, directly from complex clinical material using a combination of second generation short read and third generation nanopore sequencing technologies. The complete viral genome sequence was directly recovered from a skin lesion of a grey seal thereby preventing sequence changes due to in vitro passaging of the virus. Subsequent analysis of the proteins encoded by this virus identified genes specific for skin adaptation and pathogenesis of parapoxviruses. These data warrant the classification of seal parapoxvirus, tentatively designated SePPV, as a new species within the genus Parapoxvirus.Thomas GüntherLudwig HaasMalik AlawiPeter WohlseinJerzy MarksAdam GrundhoffPaul BecherNicole FischerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas Günther Ludwig Haas Malik Alawi Peter Wohlsein Jerzy Marks Adam Grundhoff Paul Becher Nicole Fischer Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample
description	Abstract We recovered the first full-length poxvirus genome, including the terminal hairpin region, directly from complex clinical material using a combination of second generation short read and third generation nanopore sequencing technologies. The complete viral genome sequence was directly recovered from a skin lesion of a grey seal thereby preventing sequence changes due to in vitro passaging of the virus. Subsequent analysis of the proteins encoded by this virus identified genes specific for skin adaptation and pathogenesis of parapoxviruses. These data warrant the classification of seal parapoxvirus, tentatively designated SePPV, as a new species within the genus Parapoxvirus.
format	article
author	Thomas Günther Ludwig Haas Malik Alawi Peter Wohlsein Jerzy Marks Adam Grundhoff Paul Becher Nicole Fischer
author_facet	Thomas Günther Ludwig Haas Malik Alawi Peter Wohlsein Jerzy Marks Adam Grundhoff Paul Becher Nicole Fischer
author_sort	Thomas Günther
title	Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample
title_short	Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample
title_full	Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample
title_fullStr	Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample
title_full_unstemmed	Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample
title_sort	recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/756dc6fd915c4b93802783d41f08e1ca
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Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample

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