Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils

<i>E. coli</i> cells overexpressing the enzyme atrazine chlorohydrolase were coated using layer-by-layer self-assembly. The polymeric coating was designed to improve the surface properties of the cells and create positively charged, ecologically safe, bio-hybrid capsules that can efficie...

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Autores principales:	Reut Gal, Neriya Perez-Lapid, Yael Zvulunov, Adi Radian
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polyelectrolytes polydiallyldimethylammonium chloride layer-by-layer (LbL) biodegradation herbicides pollution Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/7571411b4f584113b0d6d3b86d46f148
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id	oai:doaj.org-article:7571411b4f584113b0d6d3b86d46f148
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spelling	oai:doaj.org-article:7571411b4f584113b0d6d3b86d46f1482021-11-11T18:48:05ZLayer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils10.3390/polym132138142073-4360https://doaj.org/article/7571411b4f584113b0d6d3b86d46f1482021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3814https://doaj.org/toc/2073-4360<i>E. coli</i> cells overexpressing the enzyme atrazine chlorohydrolase were coated using layer-by-layer self-assembly. The polymeric coating was designed to improve the surface properties of the cells and create positively charged, ecologically safe, bio-hybrid capsules that can efficiently degrade the herbicide atrazine in soils. The physio-chemical properties of the bacteria/polymer interface were studied as a function of the polymeric composition of the shell and its thickness. Characterization of cell viability, enzyme activity, morphology, and size of the bio-capsules was done using fluorescence spectroscopy, BET and zeta potential measurements and electron microscopy imaging. Out of several polyelectrolytes, the combination of polydiallyldimethylammonium chloride and polysodium 4-styrenesulfonate improved the surface properties and activity of the cells to the greatest extent. The resulting bio-hybrid capsules were stable, well-dispersed, with a net positive charge and a large surface area compared to the uncoated bacteria. These non-viable, bio-hybrid capsules also exhibited a kinetic advantage in comparison with uncoated cells. When added to soils, they exhibited continuous activity over a six-week period and atrazine concentrations declined by 84%. Thus, the concept of layer-by-layer coated bacteria is a promising avenue for the design of new and sustainable bioremediation and biocatalytic platforms.Reut GalNeriya Perez-LapidYael ZvulunovAdi RadianMDPI AGarticlepolyelectrolytespolydiallyldimethylammonium chloridelayer-by-layer (LbL)biodegradationherbicidespollutionOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3814, p 3814 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyelectrolytes polydiallyldimethylammonium chloride layer-by-layer (LbL) biodegradation herbicides pollution Organic chemistry QD241-441
spellingShingle	polyelectrolytes polydiallyldimethylammonium chloride layer-by-layer (LbL) biodegradation herbicides pollution Organic chemistry QD241-441 Reut Gal Neriya Perez-Lapid Yael Zvulunov Adi Radian Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils
description	<i>E. coli</i> cells overexpressing the enzyme atrazine chlorohydrolase were coated using layer-by-layer self-assembly. The polymeric coating was designed to improve the surface properties of the cells and create positively charged, ecologically safe, bio-hybrid capsules that can efficiently degrade the herbicide atrazine in soils. The physio-chemical properties of the bacteria/polymer interface were studied as a function of the polymeric composition of the shell and its thickness. Characterization of cell viability, enzyme activity, morphology, and size of the bio-capsules was done using fluorescence spectroscopy, BET and zeta potential measurements and electron microscopy imaging. Out of several polyelectrolytes, the combination of polydiallyldimethylammonium chloride and polysodium 4-styrenesulfonate improved the surface properties and activity of the cells to the greatest extent. The resulting bio-hybrid capsules were stable, well-dispersed, with a net positive charge and a large surface area compared to the uncoated bacteria. These non-viable, bio-hybrid capsules also exhibited a kinetic advantage in comparison with uncoated cells. When added to soils, they exhibited continuous activity over a six-week period and atrazine concentrations declined by 84%. Thus, the concept of layer-by-layer coated bacteria is a promising avenue for the design of new and sustainable bioremediation and biocatalytic platforms.
format	article
author	Reut Gal Neriya Perez-Lapid Yael Zvulunov Adi Radian
author_facet	Reut Gal Neriya Perez-Lapid Yael Zvulunov Adi Radian
author_sort	Reut Gal
title	Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils
title_short	Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils
title_full	Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils
title_fullStr	Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils
title_full_unstemmed	Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils
title_sort	layer-by-layer encapsulation of herbicide-degrading bacteria for improved surface properties and compatibility in soils
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7571411b4f584113b0d6d3b86d46f148
work_keys_str_mv	AT reutgal layerbylayerencapsulationofherbicidedegradingbacteriaforimprovedsurfacepropertiesandcompatibilityinsoils AT neriyaperezlapid layerbylayerencapsulationofherbicidedegradingbacteriaforimprovedsurfacepropertiesandcompatibilityinsoils AT yaelzvulunov layerbylayerencapsulationofherbicidedegradingbacteriaforimprovedsurfacepropertiesandcompatibilityinsoils AT adiradian layerbylayerencapsulationofherbicidedegradingbacteriaforimprovedsurfacepropertiesandcompatibilityinsoils
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Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils

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