Human Monocytes/Macrophage Inflammatory Cytokine Changes Following in vivo and in vitro Schistomam manoni Infection

Mistire Wolde, 1, 2 Lisa C Laan, 3 Girmay Medhin, 1 Endalemaw Gadissa, 4 Nega Berhe, 1, 5 Aster Tsegaye 2 1Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia; 2Department of Medical Laboratory Sciences, College of Health Science, Addis Ababa University, Addis Aba...

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Autores principales:	Wolde M, Laan LC, Medhin G, Gadissa E, Berhe N, Tsegaye A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	schistosoma mansoni immune modulation monocytes macrophages Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/7578cd06e5604146a72362fd031f6d7a
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spelling	oai:doaj.org-article:7578cd06e5604146a72362fd031f6d7a2021-12-02T09:08:23ZHuman Monocytes/Macrophage Inflammatory Cytokine Changes Following in vivo and in vitro Schistomam manoni Infection1178-7031https://doaj.org/article/7578cd06e5604146a72362fd031f6d7a2020-01-01T00:00:00Zhttps://www.dovepress.com/human-monocytesmacrophage-inflammatory-cytokine-changes-following-in-v-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Mistire Wolde, 1, 2 Lisa C Laan, 3 Girmay Medhin, 1 Endalemaw Gadissa, 4 Nega Berhe, 1, 5 Aster Tsegaye 2 1Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia; 2Department of Medical Laboratory Sciences, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands; 4Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia; 5Oslo University Hospital-Ulleval, Centre for Imported and Tropical Diseases, Oslo, NorwayCorrespondence: Mistire WoldeAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, EthiopiaEmail mistire08@gmail.comIntroduction: Epidemiological and animal studies indicate that helminth infections have positive effects due to their potential to protect against autoimmune diseases. Here, we aim to assess the effect of S. mansoni infection on immune modulation of human monocytes and their potential protection against autoimmune disease development both in vivo and in vitro.Materials and Methods: Monocytes were isolated from helminth-infected Ethiopians (MHIE), and from Dutch healthy volunteers (MHV). The MHV were stimulated in vitro with S. mansoni soluble egg antigens (SEA) or soluble worm antigens (SWA). In addition, phenotypical changes were studied directly, as well as after culturing for 6 days in the presence of human serum to obtain macrophages. Q-PCR, flow cytometry, multiplex bead immunoassay, and live-cell imaging were employed during analysis.Results: MHIE showed elevated transcripts of SOCS-1 and TNF-α compared to MHV. Similarly, MHV that were stimulated with SEA demonstrated enhanced levels of SOCS-1, IL-10, and IL-12 mRNA, compared to control MHV. Remarkably, the SEA-treated monocytes showed a much higher motility than control monocytes, a hallmark of a patrolling phenotype. Furthermore, in vitro cultured macrophages that were stimulated by SEA exhibited enhanced mRNA levels of SOCS-1, IL-10, TNF-α, IL-12 and TGF-β, compared to control macrophages.Conclusion: Macrophages from MHIE as well as SEA-treated MHV show an intermediate activation phenotype with both pro-inflammatory and anti-inflammatory characteristics in vitro. The observed pro-inflammatory properties might reflect a recent response of the cells due to contact with a pathogen, whereas the anti-inflammatory properties might contribute to helminth-induced protection against inflammatory diseases. Large-scale study is recommended to consolidate the findings of the present study.Keywords: Schistosoma mansoni, immune modulation, monocytes, macrophagesWolde MLaan LCMedhin GGadissa EBerhe NTsegaye ADove Medical Pressarticleschistosoma mansoniimmune modulationmonocytesmacrophagesPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 35-43 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	schistosoma mansoni immune modulation monocytes macrophages Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	schistosoma mansoni immune modulation monocytes macrophages Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Wolde M Laan LC Medhin G Gadissa E Berhe N Tsegaye A Human Monocytes/Macrophage Inflammatory Cytokine Changes Following in vivo and in vitro Schistomam manoni Infection
description	Mistire Wolde, 1, 2 Lisa C Laan, 3 Girmay Medhin, 1 Endalemaw Gadissa, 4 Nega Berhe, 1, 5 Aster Tsegaye 2 1Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia; 2Department of Medical Laboratory Sciences, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands; 4Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia; 5Oslo University Hospital-Ulleval, Centre for Imported and Tropical Diseases, Oslo, NorwayCorrespondence: Mistire WoldeAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, EthiopiaEmail mistire08@gmail.comIntroduction: Epidemiological and animal studies indicate that helminth infections have positive effects due to their potential to protect against autoimmune diseases. Here, we aim to assess the effect of S. mansoni infection on immune modulation of human monocytes and their potential protection against autoimmune disease development both in vivo and in vitro.Materials and Methods: Monocytes were isolated from helminth-infected Ethiopians (MHIE), and from Dutch healthy volunteers (MHV). The MHV were stimulated in vitro with S. mansoni soluble egg antigens (SEA) or soluble worm antigens (SWA). In addition, phenotypical changes were studied directly, as well as after culturing for 6 days in the presence of human serum to obtain macrophages. Q-PCR, flow cytometry, multiplex bead immunoassay, and live-cell imaging were employed during analysis.Results: MHIE showed elevated transcripts of SOCS-1 and TNF-α compared to MHV. Similarly, MHV that were stimulated with SEA demonstrated enhanced levels of SOCS-1, IL-10, and IL-12 mRNA, compared to control MHV. Remarkably, the SEA-treated monocytes showed a much higher motility than control monocytes, a hallmark of a patrolling phenotype. Furthermore, in vitro cultured macrophages that were stimulated by SEA exhibited enhanced mRNA levels of SOCS-1, IL-10, TNF-α, IL-12 and TGF-β, compared to control macrophages.Conclusion: Macrophages from MHIE as well as SEA-treated MHV show an intermediate activation phenotype with both pro-inflammatory and anti-inflammatory characteristics in vitro. The observed pro-inflammatory properties might reflect a recent response of the cells due to contact with a pathogen, whereas the anti-inflammatory properties might contribute to helminth-induced protection against inflammatory diseases. Large-scale study is recommended to consolidate the findings of the present study.Keywords: Schistosoma mansoni, immune modulation, monocytes, macrophages
format	article
author	Wolde M Laan LC Medhin G Gadissa E Berhe N Tsegaye A
author_facet	Wolde M Laan LC Medhin G Gadissa E Berhe N Tsegaye A
author_sort	Wolde M
title	Human Monocytes/Macrophage Inflammatory Cytokine Changes Following in vivo and in vitro Schistomam manoni Infection
title_short	Human Monocytes/Macrophage Inflammatory Cytokine Changes Following in vivo and in vitro Schistomam manoni Infection
title_full	Human Monocytes/Macrophage Inflammatory Cytokine Changes Following in vivo and in vitro Schistomam manoni Infection
title_fullStr	Human Monocytes/Macrophage Inflammatory Cytokine Changes Following in vivo and in vitro Schistomam manoni Infection
title_full_unstemmed	Human Monocytes/Macrophage Inflammatory Cytokine Changes Following in vivo and in vitro Schistomam manoni Infection
title_sort	human monocytes/macrophage inflammatory cytokine changes following in vivo and in vitro schistomam manoni infection
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/7578cd06e5604146a72362fd031f6d7a
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Human Monocytes/Macrophage Inflammatory Cytokine Changes Following in vivo and in vitro Schistomam manoni Infection

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