Impact of deltamethrin-resistance in Aedes albopictus on its fitness cost and vector competence.

<h4>Background</h4>Aedes albopictus is one of the most invasive species in the world as well as the important vector for mosquito-borne diseases such as dengue fever, chikungunya fever and zika virus disease. Chemical control of mosquitoes is an effective method to control mosquito-borne...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jielin Deng, Yijia Guo, Xinghua Su, Shuang Liu, Wenqiang Yang, Yang Wu, Kun Wu, Guiyun Yan, Xiao-Guang Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/757a8da18efa4623801c37f9c60a2251
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:757a8da18efa4623801c37f9c60a2251
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jielin Deng Yijia Guo Xinghua Su Shuang Liu Wenqiang Yang Yang Wu Kun Wu Guiyun Yan Xiao-Guang Chen Impact of deltamethrin-resistance in Aedes albopictus on its fitness cost and vector competence.
description	<h4>Background</h4>Aedes albopictus is one of the most invasive species in the world as well as the important vector for mosquito-borne diseases such as dengue fever, chikungunya fever and zika virus disease. Chemical control of mosquitoes is an effective method to control mosquito-borne diseases, however, the wide and improper application of insecticides for vector control has led to serious resistance problems. At present, there have been many reports on the resistance to pyrethroid insecticides in vector mosquitoes including deltamethrin to Aedes albopictus. However, the fitness cost and vector competence of deltamethrin resistant Aedes albopictus remain unknown. To understand the impact of insecticide resistant mosquito is of great significance for the prevention and control mosquitoes and mosquito-borne diseases.<h4>Methodology/principal findings</h4>A laboratory resistant strain (Lab-R) of Aedes albopictus was established by deltamethrin insecticide selecting from the laboratory susceptible strain (Lab-S). The life table between the two strains were comparatively analyzed. The average development time of Lab-R and Lab-S in larvae was 9.7 days and 8.2 days (P < 0.005), and in pupae was 2.0 days and 1.8 days respectively (P > 0.05), indicating that deltamethrin resistance prolongs the larval development time of resistant mosquitoes. The average survival time of resistant adults was significantly shorter than that of susceptible adults, while the body weight of resistant female adults was significantly higher than that of the susceptible females. We also compared the vector competence for dengue virus type-2 (DENV-2) between the two strains via RT-qPCR. Considering the results of infection rate (IR) and virus load, there was no difference between the two strains during the early period of infection (4, 7, 10 day post infection (dpi)). However, in the later period of infection (14 dpi), IR and virus load in heads, salivary glands and ovaries of the resistant mosquitoes were significantly lower than those of the susceptible strain (IR of heads, salivary glands and ovaries: P < 0.05; virus load in heads and salivary glands: P < 0.05; virus load in ovaries: P < 0.001). And then, fourteen days after the DENV-2-infectious blood meal, females of the susceptible and resistant strains were allow to bite 5-day-old suckling mice. Both stains of mosquito can transmit DENV-2 to mice, but the onset of viremia was later in the mice biting by resistant group as well as lower virus copies in serum and brains, suggesting that the horizontal transmission of the resistant strain is lower than the susceptible strain. Meanwhile, we also detected IR of egg pools of the two strains on 14 dpi and found that the resistant strain were less capable of vertical transmission than susceptible mosquitoes. In addition, the average survival time of the resistant females infected with DENV-2 was 16 days, which was the shortest among the four groups of female mosquitoes, suggesting that deltamethrin resistance would shorten the life span of female Aedes albopictus infected with DENV-2.<h4>Conclusions/significance</h4>As Aedes albopictus developing high resistance to deltamethrin, the resistance prolonged the growth and development of larvae, shorten the life span of adults, as well as reduced the vector competence of resistant Aedes albopictus for DENV-2. It can be concluded that the resistance to deltamethrin in Aedes albopictus is a double-edged sword, which not only endow the mosquito survive under the pressure of insecticide, but also increase the fitness cost and decrease its vector competence. However, Aedes albopictus resistant to deltamethrin can still complete the external incubation period and transmit dengue virus, which remains a potential vector for dengue virus transmission and becomes a threat to public health. Therefore, we should pay high attention for the problem of insecticide resistance so that to better prevent and control mosquito-borne diseases.
format	article
author	Jielin Deng Yijia Guo Xinghua Su Shuang Liu Wenqiang Yang Yang Wu Kun Wu Guiyun Yan Xiao-Guang Chen
author_facet	Jielin Deng Yijia Guo Xinghua Su Shuang Liu Wenqiang Yang Yang Wu Kun Wu Guiyun Yan Xiao-Guang Chen
author_sort	Jielin Deng
title	Impact of deltamethrin-resistance in Aedes albopictus on its fitness cost and vector competence.
title_short	Impact of deltamethrin-resistance in Aedes albopictus on its fitness cost and vector competence.
title_full	Impact of deltamethrin-resistance in Aedes albopictus on its fitness cost and vector competence.
title_fullStr	Impact of deltamethrin-resistance in Aedes albopictus on its fitness cost and vector competence.
title_full_unstemmed	Impact of deltamethrin-resistance in Aedes albopictus on its fitness cost and vector competence.
title_sort	impact of deltamethrin-resistance in aedes albopictus on its fitness cost and vector competence.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/757a8da18efa4623801c37f9c60a2251
work_keys_str_mv	AT jielindeng impactofdeltamethrinresistanceinaedesalbopictusonitsfitnesscostandvectorcompetence AT yijiaguo impactofdeltamethrinresistanceinaedesalbopictusonitsfitnesscostandvectorcompetence AT xinghuasu impactofdeltamethrinresistanceinaedesalbopictusonitsfitnesscostandvectorcompetence AT shuangliu impactofdeltamethrinresistanceinaedesalbopictusonitsfitnesscostandvectorcompetence AT wenqiangyang impactofdeltamethrinresistanceinaedesalbopictusonitsfitnesscostandvectorcompetence AT yangwu impactofdeltamethrinresistanceinaedesalbopictusonitsfitnesscostandvectorcompetence AT kunwu impactofdeltamethrinresistanceinaedesalbopictusonitsfitnesscostandvectorcompetence AT guiyunyan impactofdeltamethrinresistanceinaedesalbopictusonitsfitnesscostandvectorcompetence AT xiaoguangchen impactofdeltamethrinresistanceinaedesalbopictusonitsfitnesscostandvectorcompetence
_version_	1718374113964195840
spelling	oai:doaj.org-article:757a8da18efa4623801c37f9c60a22512021-12-02T20:23:25ZImpact of deltamethrin-resistance in Aedes albopictus on its fitness cost and vector competence.1935-27271935-273510.1371/journal.pntd.0009391https://doaj.org/article/757a8da18efa4623801c37f9c60a22512021-04-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009391https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Aedes albopictus is one of the most invasive species in the world as well as the important vector for mosquito-borne diseases such as dengue fever, chikungunya fever and zika virus disease. Chemical control of mosquitoes is an effective method to control mosquito-borne diseases, however, the wide and improper application of insecticides for vector control has led to serious resistance problems. At present, there have been many reports on the resistance to pyrethroid insecticides in vector mosquitoes including deltamethrin to Aedes albopictus. However, the fitness cost and vector competence of deltamethrin resistant Aedes albopictus remain unknown. To understand the impact of insecticide resistant mosquito is of great significance for the prevention and control mosquitoes and mosquito-borne diseases.<h4>Methodology/principal findings</h4>A laboratory resistant strain (Lab-R) of Aedes albopictus was established by deltamethrin insecticide selecting from the laboratory susceptible strain (Lab-S). The life table between the two strains were comparatively analyzed. The average development time of Lab-R and Lab-S in larvae was 9.7 days and 8.2 days (P < 0.005), and in pupae was 2.0 days and 1.8 days respectively (P > 0.05), indicating that deltamethrin resistance prolongs the larval development time of resistant mosquitoes. The average survival time of resistant adults was significantly shorter than that of susceptible adults, while the body weight of resistant female adults was significantly higher than that of the susceptible females. We also compared the vector competence for dengue virus type-2 (DENV-2) between the two strains via RT-qPCR. Considering the results of infection rate (IR) and virus load, there was no difference between the two strains during the early period of infection (4, 7, 10 day post infection (dpi)). However, in the later period of infection (14 dpi), IR and virus load in heads, salivary glands and ovaries of the resistant mosquitoes were significantly lower than those of the susceptible strain (IR of heads, salivary glands and ovaries: P < 0.05; virus load in heads and salivary glands: P < 0.05; virus load in ovaries: P < 0.001). And then, fourteen days after the DENV-2-infectious blood meal, females of the susceptible and resistant strains were allow to bite 5-day-old suckling mice. Both stains of mosquito can transmit DENV-2 to mice, but the onset of viremia was later in the mice biting by resistant group as well as lower virus copies in serum and brains, suggesting that the horizontal transmission of the resistant strain is lower than the susceptible strain. Meanwhile, we also detected IR of egg pools of the two strains on 14 dpi and found that the resistant strain were less capable of vertical transmission than susceptible mosquitoes. In addition, the average survival time of the resistant females infected with DENV-2 was 16 days, which was the shortest among the four groups of female mosquitoes, suggesting that deltamethrin resistance would shorten the life span of female Aedes albopictus infected with DENV-2.<h4>Conclusions/significance</h4>As Aedes albopictus developing high resistance to deltamethrin, the resistance prolonged the growth and development of larvae, shorten the life span of adults, as well as reduced the vector competence of resistant Aedes albopictus for DENV-2. It can be concluded that the resistance to deltamethrin in Aedes albopictus is a double-edged sword, which not only endow the mosquito survive under the pressure of insecticide, but also increase the fitness cost and decrease its vector competence. However, Aedes albopictus resistant to deltamethrin can still complete the external incubation period and transmit dengue virus, which remains a potential vector for dengue virus transmission and becomes a threat to public health. Therefore, we should pay high attention for the problem of insecticide resistance so that to better prevent and control mosquito-borne diseases.Jielin DengYijia GuoXinghua SuShuang LiuWenqiang YangYang WuKun WuGuiyun YanXiao-Guang ChenPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 4, p e0009391 (2021)

Impact of deltamethrin-resistance in Aedes albopictus on its fitness cost and vector competence.

Ejemplares similares