Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination.

Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate double strand breakage and exchange. A recombina...

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Autores principales:	Lin Zhang, Binyan Zhu, Ruixue Dai, Guoping Zhao, Xiaoming Ding
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/757b02e592664353aa9512fdb1da41e5
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spelling	oai:doaj.org-article:757b02e592664353aa9512fdb1da41e52021-11-18T08:45:13ZControl of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination.1932-620310.1371/journal.pone.0080434https://doaj.org/article/757b02e592664353aa9512fdb1da41e52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278283/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate double strand breakage and exchange. A recombination directionality factor (RDF) is commonly required for switching integration to excision. Here we report the characterization of the RDF protein for φBT1 recombination. The RDF, is a phage-encoded gp3 gene product (28 KDa), which allows efficient active excision between attL and attR, and inhibits integration between attB and attP; Gp3 can also catalyze topological relaxation with the integrase of supercoiled plasmids containing a single excision site. Further study showed that Gp3 could form a dimer and interact with the integrase whether it bound to the substrate or not. The synapse formation of attL or attR alone with integrase and Gp3 showed that synapsis did not discriminate between the two sites, indicating that complementarity of central dinucleotides is the sole determinant of outcome in correct excision synapses. Furthermore, both in vitro and in vivo evidence support that the RDFs of φBT1 and φC31 were fully exchangeable, despite the low amino acid sequence identity of the two integrases.Lin ZhangBinyan ZhuRuixue DaiGuoping ZhaoXiaoming DingPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80434 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lin Zhang Binyan Zhu Ruixue Dai Guoping Zhao Xiaoming Ding Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination.
description	Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate double strand breakage and exchange. A recombination directionality factor (RDF) is commonly required for switching integration to excision. Here we report the characterization of the RDF protein for φBT1 recombination. The RDF, is a phage-encoded gp3 gene product (28 KDa), which allows efficient active excision between attL and attR, and inhibits integration between attB and attP; Gp3 can also catalyze topological relaxation with the integrase of supercoiled plasmids containing a single excision site. Further study showed that Gp3 could form a dimer and interact with the integrase whether it bound to the substrate or not. The synapse formation of attL or attR alone with integrase and Gp3 showed that synapsis did not discriminate between the two sites, indicating that complementarity of central dinucleotides is the sole determinant of outcome in correct excision synapses. Furthermore, both in vitro and in vivo evidence support that the RDFs of φBT1 and φC31 were fully exchangeable, despite the low amino acid sequence identity of the two integrases.
format	article
author	Lin Zhang Binyan Zhu Ruixue Dai Guoping Zhao Xiaoming Ding
author_facet	Lin Zhang Binyan Zhu Ruixue Dai Guoping Zhao Xiaoming Ding
author_sort	Lin Zhang
title	Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination.
title_short	Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination.
title_full	Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination.
title_fullStr	Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination.
title_full_unstemmed	Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination.
title_sort	control of directionality in streptomyces phage φbt1 integrase-mediated site-specific recombination.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/757b02e592664353aa9512fdb1da41e5
work_keys_str_mv	AT linzhang controlofdirectionalityinstreptomycesphagephbt1integrasemediatedsitespecificrecombination AT binyanzhu controlofdirectionalityinstreptomycesphagephbt1integrasemediatedsitespecificrecombination AT ruixuedai controlofdirectionalityinstreptomycesphagephbt1integrasemediatedsitespecificrecombination AT guopingzhao controlofdirectionalityinstreptomycesphagephbt1integrasemediatedsitespecificrecombination AT xiaomingding controlofdirectionalityinstreptomycesphagephbt1integrasemediatedsitespecificrecombination
_version_	1718421390103674880

Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination.

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