Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis

Uveitis describes a heterogeneous group of inflammatory eye diseases characterized by infiltration of leukocytes into the uveal tissues. Uveitis associated with the HLA haplotype B27 (HLA-B27) is a common subtype of uveitis and a prototypical ocular immune-mediated disease. Local immune mechanisms d...

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Autores principales:	Maren Kasper, Michael Heming, David Schafflick, Xiaolin Li, Tobias Lautwein, Melissa Meyer zu Horste, Dirk Bauer, Karoline Walscheid, Heinz Wiendl, Karin Loser, Arnd Heiligenhaus, Gerd Meyer zu Hörste
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	HLA-B27 uveitis inflammation dendritic cells Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/757c955af77f4ad394c56bea4c8ae6a8
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spelling	oai:doaj.org-article:757c955af77f4ad394c56bea4c8ae6a82021-11-16T12:40:17ZIntraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis10.7554/eLife.673962050-084Xe67396https://doaj.org/article/757c955af77f4ad394c56bea4c8ae6a82021-11-01T00:00:00Zhttps://elifesciences.org/articles/67396https://doaj.org/toc/2050-084XUveitis describes a heterogeneous group of inflammatory eye diseases characterized by infiltration of leukocytes into the uveal tissues. Uveitis associated with the HLA haplotype B27 (HLA-B27) is a common subtype of uveitis and a prototypical ocular immune-mediated disease. Local immune mechanisms driving human uveitis are poorly characterized mainly due to the limited available biomaterial and subsequent technical limitations. Here, we provide the first high-resolution characterization of intraocular leukocytes in HLA-B27-positive (n = 4) and -negative (n = 2) anterior uveitis and an infectious endophthalmitis control (n = 1) by combining single-cell RNA-sequencing with flow cytometry and protein analysis. Ocular cell infiltrates consisted primarily of lymphocytes in both subtypes of uveitis and of myeloid cells in infectious endophthalmitis. HLA-B27-positive uveitis exclusively featured a plasmacytoid and classical dendritic cell (cDC) infiltrate. Moreover, cDCs were central in predicted local cell-cell communication. This suggests a unique pattern of ocular leukocyte infiltration in HLA-B27-positive uveitis with relevance to DCs.Maren KasperMichael HemingDavid SchafflickXiaolin LiTobias LautweinMelissa Meyer zu HorsteDirk BauerKaroline WalscheidHeinz WiendlKarin LoserArnd HeiligenhausGerd Meyer zu HörsteeLife Sciences Publications LtdarticleHLA-B27uveitisinflammationdendritic cellsMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HLA-B27 uveitis inflammation dendritic cells Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	HLA-B27 uveitis inflammation dendritic cells Medicine R Science Q Biology (General) QH301-705.5 Maren Kasper Michael Heming David Schafflick Xiaolin Li Tobias Lautwein Melissa Meyer zu Horste Dirk Bauer Karoline Walscheid Heinz Wiendl Karin Loser Arnd Heiligenhaus Gerd Meyer zu Hörste Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis
description	Uveitis describes a heterogeneous group of inflammatory eye diseases characterized by infiltration of leukocytes into the uveal tissues. Uveitis associated with the HLA haplotype B27 (HLA-B27) is a common subtype of uveitis and a prototypical ocular immune-mediated disease. Local immune mechanisms driving human uveitis are poorly characterized mainly due to the limited available biomaterial and subsequent technical limitations. Here, we provide the first high-resolution characterization of intraocular leukocytes in HLA-B27-positive (n = 4) and -negative (n = 2) anterior uveitis and an infectious endophthalmitis control (n = 1) by combining single-cell RNA-sequencing with flow cytometry and protein analysis. Ocular cell infiltrates consisted primarily of lymphocytes in both subtypes of uveitis and of myeloid cells in infectious endophthalmitis. HLA-B27-positive uveitis exclusively featured a plasmacytoid and classical dendritic cell (cDC) infiltrate. Moreover, cDCs were central in predicted local cell-cell communication. This suggests a unique pattern of ocular leukocyte infiltration in HLA-B27-positive uveitis with relevance to DCs.
format	article
author	Maren Kasper Michael Heming David Schafflick Xiaolin Li Tobias Lautwein Melissa Meyer zu Horste Dirk Bauer Karoline Walscheid Heinz Wiendl Karin Loser Arnd Heiligenhaus Gerd Meyer zu Hörste
author_facet	Maren Kasper Michael Heming David Schafflick Xiaolin Li Tobias Lautwein Melissa Meyer zu Horste Dirk Bauer Karoline Walscheid Heinz Wiendl Karin Loser Arnd Heiligenhaus Gerd Meyer zu Hörste
author_sort	Maren Kasper
title	Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis
title_short	Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis
title_full	Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis
title_fullStr	Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis
title_full_unstemmed	Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis
title_sort	intraocular dendritic cells characterize hla-b27-associated acute anterior uveitis
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/757c955af77f4ad394c56bea4c8ae6a8
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_version_	1718426555176189952

Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis

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