Mutation in <i>OsFWL7</i> Affects Cadmium and Micronutrient Metal Accumulation in Rice

Micronutrient metals, such as Mn, Cu, Fe, and Zn, are essential heavy metals for plant growth and development, while Cd is a nonessential heavy metal that is highly toxic to both plants and humans. Our understanding of the molecular mechanisms underlying Cd and micronutrient metal accumulation in pl...

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Autores principales:	Qingsong Gao, Lei Liu, Haiying Zhou, Xi Liu, Wei Li, Yu Min, Yurong Yan, Jianhui Ji, Hao Zhang, Xiangxiang Zhao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rice <i>FW2.2</i>-like gene cadmium micronutrient metal protein interaction membrane microdomain Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/75804294bdb941e496e99427c68c224b
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spelling	oai:doaj.org-article:75804294bdb941e496e99427c68c224b2021-11-25T17:58:11ZMutation in <i>OsFWL7</i> Affects Cadmium and Micronutrient Metal Accumulation in Rice10.3390/ijms2222125831422-00671661-6596https://doaj.org/article/75804294bdb941e496e99427c68c224b2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12583https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Micronutrient metals, such as Mn, Cu, Fe, and Zn, are essential heavy metals for plant growth and development, while Cd is a nonessential heavy metal that is highly toxic to both plants and humans. Our understanding of the molecular mechanisms underlying Cd and micronutrient metal accumulation in plants remains incomplete. Here, we show that <i>OsFWL7</i>, an <i>FW2.2</i>-like (<i>FWL</i>) family gene in <i>Oryza sativa</i>, is preferentially expressed in the root and encodes a protein localized to the cell membrane. The <i>osfwl7</i> mutation reduces both the uptake and the root-to-shoot translocation of Cd in rice plants. Additionally, the accumulation of micronutrient metals, including Mn, Cu, and Fe, was lower in <i>osfwl7</i> mutants than in the wildtype plants under normal growth conditions. Moreover, the <i>osfwl7</i> mutation affects the expression of several heavy metal transporter genes. Protein interaction analyses reveal that rice FWL proteins interact with themselves and one another, and with several membrane microdomain marker proteins. Our results suggest that <i>OsFWL7</i> is involved in Cd and micronutrient metal accumulation in rice. Additionally, rice FWL proteins may form oligomers and some of them may be located in membrane microdomains.Qingsong GaoLei LiuHaiying ZhouXi LiuWei LiYu MinYurong YanJianhui JiHao ZhangXiangxiang ZhaoMDPI AGarticlerice<i>FW2.2</i>-like genecadmiummicronutrient metalprotein interactionmembrane microdomainBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12583, p 12583 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rice <i>FW2.2</i>-like gene cadmium micronutrient metal protein interaction membrane microdomain Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	rice <i>FW2.2</i>-like gene cadmium micronutrient metal protein interaction membrane microdomain Biology (General) QH301-705.5 Chemistry QD1-999 Qingsong Gao Lei Liu Haiying Zhou Xi Liu Wei Li Yu Min Yurong Yan Jianhui Ji Hao Zhang Xiangxiang Zhao Mutation in <i>OsFWL7</i> Affects Cadmium and Micronutrient Metal Accumulation in Rice
description	Micronutrient metals, such as Mn, Cu, Fe, and Zn, are essential heavy metals for plant growth and development, while Cd is a nonessential heavy metal that is highly toxic to both plants and humans. Our understanding of the molecular mechanisms underlying Cd and micronutrient metal accumulation in plants remains incomplete. Here, we show that <i>OsFWL7</i>, an <i>FW2.2</i>-like (<i>FWL</i>) family gene in <i>Oryza sativa</i>, is preferentially expressed in the root and encodes a protein localized to the cell membrane. The <i>osfwl7</i> mutation reduces both the uptake and the root-to-shoot translocation of Cd in rice plants. Additionally, the accumulation of micronutrient metals, including Mn, Cu, and Fe, was lower in <i>osfwl7</i> mutants than in the wildtype plants under normal growth conditions. Moreover, the <i>osfwl7</i> mutation affects the expression of several heavy metal transporter genes. Protein interaction analyses reveal that rice FWL proteins interact with themselves and one another, and with several membrane microdomain marker proteins. Our results suggest that <i>OsFWL7</i> is involved in Cd and micronutrient metal accumulation in rice. Additionally, rice FWL proteins may form oligomers and some of them may be located in membrane microdomains.
format	article
author	Qingsong Gao Lei Liu Haiying Zhou Xi Liu Wei Li Yu Min Yurong Yan Jianhui Ji Hao Zhang Xiangxiang Zhao
author_facet	Qingsong Gao Lei Liu Haiying Zhou Xi Liu Wei Li Yu Min Yurong Yan Jianhui Ji Hao Zhang Xiangxiang Zhao
author_sort	Qingsong Gao
title	Mutation in <i>OsFWL7</i> Affects Cadmium and Micronutrient Metal Accumulation in Rice
title_short	Mutation in <i>OsFWL7</i> Affects Cadmium and Micronutrient Metal Accumulation in Rice
title_full	Mutation in <i>OsFWL7</i> Affects Cadmium and Micronutrient Metal Accumulation in Rice
title_fullStr	Mutation in <i>OsFWL7</i> Affects Cadmium and Micronutrient Metal Accumulation in Rice
title_full_unstemmed	Mutation in <i>OsFWL7</i> Affects Cadmium and Micronutrient Metal Accumulation in Rice
title_sort	mutation in <i>osfwl7</i> affects cadmium and micronutrient metal accumulation in rice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/75804294bdb941e496e99427c68c224b
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Mutation in <i>OsFWL7</i> Affects Cadmium and Micronutrient Metal Accumulation in Rice

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