Screening of biocontrol bacteria against soft rot disease of Colocasia esculenta (L.) schott and its field application.

Soft rot disease is a major pathogenic bacteria of Fuding areca taro and has caused serious losses. This study aims to screen biocontrol bacterial against soft rot disease. A total of 53 bacterial strains were isolated from the rhizosphere soil, nine of which exhibited good biocontrol effect against...

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Autores principales:	Xiaofei Dong, Lu Fang, Zuyun Ye, Guangqiang Zhu, Qianyu Lai, Shengrong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/75813299d73c4c7fa9cf1f809e86823c
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spelling	oai:doaj.org-article:75813299d73c4c7fa9cf1f809e86823c2021-12-02T20:09:15ZScreening of biocontrol bacteria against soft rot disease of Colocasia esculenta (L.) schott and its field application.1932-620310.1371/journal.pone.0254070https://doaj.org/article/75813299d73c4c7fa9cf1f809e86823c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254070https://doaj.org/toc/1932-6203Soft rot disease is a major pathogenic bacteria of Fuding areca taro and has caused serious losses. This study aims to screen biocontrol bacterial against soft rot disease. A total of 53 bacterial strains were isolated from the rhizosphere soil, nine of which exhibited good biocontrol effect against the pathogenic bacteria of soft rot disease as seen in antagonistic screening of biocontrol bacteria from corm in vitro. Strains were selected by physical and chemical experiments, biocontrol effect tests in vivo, molecular sequencing, morphological observation and field tests. Four strains including CAB-L005, CAB-L012, CAB-L014, and CAB-L022 exhibited strong antagonistic effects. On the basis of the sequence homology of 16S rRNA genes, the similarity between strain CAB-L005 and Bacillus tropicus was 100%, that between strain CAB-L012 and Bacillus subtilis was 99%, and that between strain CAB-L014 and Bacillus tequilensis was 100%, and similarity between strain CAB-L022 and Bacillus cereus was 100%. The isolated bacteria demonstrated good biocontrol effects in field experiments. In this study, four strains with good biocontrol application value were isolated and identified, providing a foundation for biocontrol against soft rot disease in areca taro.Xiaofei DongLu FangZuyun YeGuangqiang ZhuQianyu LaiShengrong LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254070 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaofei Dong Lu Fang Zuyun Ye Guangqiang Zhu Qianyu Lai Shengrong Liu Screening of biocontrol bacteria against soft rot disease of Colocasia esculenta (L.) schott and its field application.
description	Soft rot disease is a major pathogenic bacteria of Fuding areca taro and has caused serious losses. This study aims to screen biocontrol bacterial against soft rot disease. A total of 53 bacterial strains were isolated from the rhizosphere soil, nine of which exhibited good biocontrol effect against the pathogenic bacteria of soft rot disease as seen in antagonistic screening of biocontrol bacteria from corm in vitro. Strains were selected by physical and chemical experiments, biocontrol effect tests in vivo, molecular sequencing, morphological observation and field tests. Four strains including CAB-L005, CAB-L012, CAB-L014, and CAB-L022 exhibited strong antagonistic effects. On the basis of the sequence homology of 16S rRNA genes, the similarity between strain CAB-L005 and Bacillus tropicus was 100%, that between strain CAB-L012 and Bacillus subtilis was 99%, and that between strain CAB-L014 and Bacillus tequilensis was 100%, and similarity between strain CAB-L022 and Bacillus cereus was 100%. The isolated bacteria demonstrated good biocontrol effects in field experiments. In this study, four strains with good biocontrol application value were isolated and identified, providing a foundation for biocontrol against soft rot disease in areca taro.
format	article
author	Xiaofei Dong Lu Fang Zuyun Ye Guangqiang Zhu Qianyu Lai Shengrong Liu
author_facet	Xiaofei Dong Lu Fang Zuyun Ye Guangqiang Zhu Qianyu Lai Shengrong Liu
author_sort	Xiaofei Dong
title	Screening of biocontrol bacteria against soft rot disease of Colocasia esculenta (L.) schott and its field application.
title_short	Screening of biocontrol bacteria against soft rot disease of Colocasia esculenta (L.) schott and its field application.
title_full	Screening of biocontrol bacteria against soft rot disease of Colocasia esculenta (L.) schott and its field application.
title_fullStr	Screening of biocontrol bacteria against soft rot disease of Colocasia esculenta (L.) schott and its field application.
title_full_unstemmed	Screening of biocontrol bacteria against soft rot disease of Colocasia esculenta (L.) schott and its field application.
title_sort	screening of biocontrol bacteria against soft rot disease of colocasia esculenta (l.) schott and its field application.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/75813299d73c4c7fa9cf1f809e86823c
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Screening of biocontrol bacteria against soft rot disease of Colocasia esculenta (L.) schott and its field application.

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