Probing Hepatic Glucose Metabolism via <sup>13</sup>C NMR Spectroscopy in Perfused Livers—Applications to Drug Development

Despite being first published over 40 years ago, the combination of <sup>13</sup>C nuclear magnetic resonance spectroscopy (NMR) and the isolated perfused liver preparation remains a unique and relevant approach in investigating the effects of pharmacological interventions on hepatic met...

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Autores principales:	Corin O. Miller, Jin Cao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	perfused liver nuclear magnetic resonance spectroscopy NMR MRS stable isotope <sup>13</sup>C (carbon-13) Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7581fb343feb4fdfb2e25c87b5cab33e
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id	oai:doaj.org-article:7581fb343feb4fdfb2e25c87b5cab33e
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spelling	oai:doaj.org-article:7581fb343feb4fdfb2e25c87b5cab33e2021-11-25T18:20:22ZProbing Hepatic Glucose Metabolism via <sup>13</sup>C NMR Spectroscopy in Perfused Livers—Applications to Drug Development10.3390/metabo111107122218-1989https://doaj.org/article/7581fb343feb4fdfb2e25c87b5cab33e2021-10-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/712https://doaj.org/toc/2218-1989Despite being first published over 40 years ago, the combination of <sup>13</sup>C nuclear magnetic resonance spectroscopy (NMR) and the isolated perfused liver preparation remains a unique and relevant approach in investigating the effects of pharmacological interventions on hepatic metabolism. The use of intact, perfused livers maintains many metabolic reactions at their respective rates in vivo, while the use of <sup>13</sup>C-labelled substrates in combination with <sup>13</sup>C NMR allows for a detailed study of specific pathways, as well as the design of robust assays which can be used to evaluate novel pharmacological agents. In this review article, we share some of the methods used to probe glucose metabolism, and highlight key findings and successes derived from the application of this specialized technique to the area of drug development for diabetes and related metabolic disorders.Corin O. MillerJin CaoMDPI AGarticleperfused livernuclear magnetic resonance spectroscopyNMRMRSstable isotope<sup>13</sup>C (carbon-13)MicrobiologyQR1-502ENMetabolites, Vol 11, Iss 712, p 712 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	perfused liver nuclear magnetic resonance spectroscopy NMR MRS stable isotope <sup>13</sup>C (carbon-13) Microbiology QR1-502
spellingShingle	perfused liver nuclear magnetic resonance spectroscopy NMR MRS stable isotope <sup>13</sup>C (carbon-13) Microbiology QR1-502 Corin O. Miller Jin Cao Probing Hepatic Glucose Metabolism via <sup>13</sup>C NMR Spectroscopy in Perfused Livers—Applications to Drug Development
description	Despite being first published over 40 years ago, the combination of <sup>13</sup>C nuclear magnetic resonance spectroscopy (NMR) and the isolated perfused liver preparation remains a unique and relevant approach in investigating the effects of pharmacological interventions on hepatic metabolism. The use of intact, perfused livers maintains many metabolic reactions at their respective rates in vivo, while the use of <sup>13</sup>C-labelled substrates in combination with <sup>13</sup>C NMR allows for a detailed study of specific pathways, as well as the design of robust assays which can be used to evaluate novel pharmacological agents. In this review article, we share some of the methods used to probe glucose metabolism, and highlight key findings and successes derived from the application of this specialized technique to the area of drug development for diabetes and related metabolic disorders.
format	article
author	Corin O. Miller Jin Cao
author_facet	Corin O. Miller Jin Cao
author_sort	Corin O. Miller
title	Probing Hepatic Glucose Metabolism via <sup>13</sup>C NMR Spectroscopy in Perfused Livers—Applications to Drug Development
title_short	Probing Hepatic Glucose Metabolism via <sup>13</sup>C NMR Spectroscopy in Perfused Livers—Applications to Drug Development
title_full	Probing Hepatic Glucose Metabolism via <sup>13</sup>C NMR Spectroscopy in Perfused Livers—Applications to Drug Development
title_fullStr	Probing Hepatic Glucose Metabolism via <sup>13</sup>C NMR Spectroscopy in Perfused Livers—Applications to Drug Development
title_full_unstemmed	Probing Hepatic Glucose Metabolism via <sup>13</sup>C NMR Spectroscopy in Perfused Livers—Applications to Drug Development
title_sort	probing hepatic glucose metabolism via <sup>13</sup>c nmr spectroscopy in perfused livers—applications to drug development
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7581fb343feb4fdfb2e25c87b5cab33e
work_keys_str_mv	AT corinomiller probinghepaticglucosemetabolismviasup13supcnmrspectroscopyinperfusedliversapplicationstodrugdevelopment AT jincao probinghepaticglucosemetabolismviasup13supcnmrspectroscopyinperfusedliversapplicationstodrugdevelopment
_version_	1718411327448285184

Probing Hepatic Glucose Metabolism via <sup>13</sup>C NMR Spectroscopy in Perfused Livers—Applications to Drug Development

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