A new BiofilmChip device for testing biofilm formation and antibiotic susceptibility

Abstract Currently, three major circumstances threaten the management of bacterial infections: increasing antimicrobial resistance, expansion of chronic biofilm-associated infections, and lack of an appropriate approach to treat them. To date, the development of accelerated drug susceptibility testi...

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Autores principales:	Núria Blanco-Cabra, Maria José López-Martínez, Betsy Verónica Arévalo-Jaimes, María Teresa Martin-Gómez, Josep Samitier, Eduard Torrents
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/758f4027175b472db225f40d78bb7777
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spelling	oai:doaj.org-article:758f4027175b472db225f40d78bb77772021-12-02T16:34:05ZA new BiofilmChip device for testing biofilm formation and antibiotic susceptibility10.1038/s41522-021-00236-12055-5008https://doaj.org/article/758f4027175b472db225f40d78bb77772021-08-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00236-1https://doaj.org/toc/2055-5008Abstract Currently, three major circumstances threaten the management of bacterial infections: increasing antimicrobial resistance, expansion of chronic biofilm-associated infections, and lack of an appropriate approach to treat them. To date, the development of accelerated drug susceptibility testing of biofilms and of new antibiofouling systems has not been achieved despite the availability of different methodologies. There is a need for easy-to-use methods of testing the antibiotic susceptibility of bacteria that form biofilms and for screening new possible antibiofilm strategies. Herein, we present a microfluidic platform with an integrated interdigitated sensor (BiofilmChip). This new device allows an irreversible and homogeneous attachment of bacterial cells of clinical origin, even directly from clinical specimens, and the biofilms grown can be monitored by confocal microscopy or electrical impedance spectroscopy. The device proved to be suitable to study polymicrobial communities, as well as to measure the effect of antimicrobials on biofilms without introducing disturbances due to manipulation, thus better mimicking real-life clinical situations. Our results demonstrate that BiofilmChip is a straightforward tool for antimicrobial biofilm susceptibility testing that could be easily implemented in routine clinical laboratories.Núria Blanco-CabraMaria José López-MartínezBetsy Verónica Arévalo-JaimesMaría Teresa Martin-GómezJosep SamitierEduard TorrentsNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Núria Blanco-Cabra Maria José López-Martínez Betsy Verónica Arévalo-Jaimes María Teresa Martin-Gómez Josep Samitier Eduard Torrents A new BiofilmChip device for testing biofilm formation and antibiotic susceptibility
description	Abstract Currently, three major circumstances threaten the management of bacterial infections: increasing antimicrobial resistance, expansion of chronic biofilm-associated infections, and lack of an appropriate approach to treat them. To date, the development of accelerated drug susceptibility testing of biofilms and of new antibiofouling systems has not been achieved despite the availability of different methodologies. There is a need for easy-to-use methods of testing the antibiotic susceptibility of bacteria that form biofilms and for screening new possible antibiofilm strategies. Herein, we present a microfluidic platform with an integrated interdigitated sensor (BiofilmChip). This new device allows an irreversible and homogeneous attachment of bacterial cells of clinical origin, even directly from clinical specimens, and the biofilms grown can be monitored by confocal microscopy or electrical impedance spectroscopy. The device proved to be suitable to study polymicrobial communities, as well as to measure the effect of antimicrobials on biofilms without introducing disturbances due to manipulation, thus better mimicking real-life clinical situations. Our results demonstrate that BiofilmChip is a straightforward tool for antimicrobial biofilm susceptibility testing that could be easily implemented in routine clinical laboratories.
format	article
author	Núria Blanco-Cabra Maria José López-Martínez Betsy Verónica Arévalo-Jaimes María Teresa Martin-Gómez Josep Samitier Eduard Torrents
author_facet	Núria Blanco-Cabra Maria José López-Martínez Betsy Verónica Arévalo-Jaimes María Teresa Martin-Gómez Josep Samitier Eduard Torrents
author_sort	Núria Blanco-Cabra
title	A new BiofilmChip device for testing biofilm formation and antibiotic susceptibility
title_short	A new BiofilmChip device for testing biofilm formation and antibiotic susceptibility
title_full	A new BiofilmChip device for testing biofilm formation and antibiotic susceptibility
title_fullStr	A new BiofilmChip device for testing biofilm formation and antibiotic susceptibility
title_full_unstemmed	A new BiofilmChip device for testing biofilm formation and antibiotic susceptibility
title_sort	new biofilmchip device for testing biofilm formation and antibiotic susceptibility
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/758f4027175b472db225f40d78bb7777
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A new BiofilmChip device for testing biofilm formation and antibiotic susceptibility

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