Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

Cell-free gene expression systems are an attractive platform for biomanufacturing and synthetic biology. Here the authors characterize native membrane vesicles in E. coli extracts for improved glycoengineering.

Guardado en:
Detalles Bibliográficos
Autores principales: Jasmine M. Hershewe, Katherine F. Warfel, Shaelyn M. Iyer, Justin A. Peruzzi, Claretta J. Sullivan, Eric W. Roth, Matthew P. DeLisa, Neha P. Kamat, Michael C. Jewett
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Q
Acceso en línea:https://doaj.org/article/7590e16edeb24beca41ddcd5cdb6b1c9
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:7590e16edeb24beca41ddcd5cdb6b1c9
record_format dspace
spelling oai:doaj.org-article:7590e16edeb24beca41ddcd5cdb6b1c92021-12-02T17:33:40ZImproving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles10.1038/s41467-021-22329-32041-1723https://doaj.org/article/7590e16edeb24beca41ddcd5cdb6b1c92021-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22329-3https://doaj.org/toc/2041-1723Cell-free gene expression systems are an attractive platform for biomanufacturing and synthetic biology. Here the authors characterize native membrane vesicles in E. coli extracts for improved glycoengineering.Jasmine M. HersheweKatherine F. WarfelShaelyn M. IyerJustin A. PeruzziClaretta J. SullivanEric W. RothMatthew P. DeLisaNeha P. KamatMichael C. JewettNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Jasmine M. Hershewe
Katherine F. Warfel
Shaelyn M. Iyer
Justin A. Peruzzi
Claretta J. Sullivan
Eric W. Roth
Matthew P. DeLisa
Neha P. Kamat
Michael C. Jewett
Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
description Cell-free gene expression systems are an attractive platform for biomanufacturing and synthetic biology. Here the authors characterize native membrane vesicles in E. coli extracts for improved glycoengineering.
format article
author Jasmine M. Hershewe
Katherine F. Warfel
Shaelyn M. Iyer
Justin A. Peruzzi
Claretta J. Sullivan
Eric W. Roth
Matthew P. DeLisa
Neha P. Kamat
Michael C. Jewett
author_facet Jasmine M. Hershewe
Katherine F. Warfel
Shaelyn M. Iyer
Justin A. Peruzzi
Claretta J. Sullivan
Eric W. Roth
Matthew P. DeLisa
Neha P. Kamat
Michael C. Jewett
author_sort Jasmine M. Hershewe
title Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
title_short Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
title_full Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
title_fullStr Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
title_full_unstemmed Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
title_sort improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/7590e16edeb24beca41ddcd5cdb6b1c9
work_keys_str_mv AT jasminemhershewe improvingcellfreeglycoproteinsynthesisbycharacterizingandenrichingnativemembranevesicles
AT katherinefwarfel improvingcellfreeglycoproteinsynthesisbycharacterizingandenrichingnativemembranevesicles
AT shaelynmiyer improvingcellfreeglycoproteinsynthesisbycharacterizingandenrichingnativemembranevesicles
AT justinaperuzzi improvingcellfreeglycoproteinsynthesisbycharacterizingandenrichingnativemembranevesicles
AT clarettajsullivan improvingcellfreeglycoproteinsynthesisbycharacterizingandenrichingnativemembranevesicles
AT ericwroth improvingcellfreeglycoproteinsynthesisbycharacterizingandenrichingnativemembranevesicles
AT matthewpdelisa improvingcellfreeglycoproteinsynthesisbycharacterizingandenrichingnativemembranevesicles
AT nehapkamat improvingcellfreeglycoproteinsynthesisbycharacterizingandenrichingnativemembranevesicles
AT michaelcjewett improvingcellfreeglycoproteinsynthesisbycharacterizingandenrichingnativemembranevesicles
_version_ 1718379947944312832