Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs

Control of CRISPR-Cas9 activity allows for fine-tuning of editing and gene expression. Here the authors use gRNAs modified with RNA aptamers to enable small molecule control in bacterial systems.

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Autores principales:	Kale Kundert, James E. Lucas, Kyle E. Watters, Christof Fellmann, Andrew H. Ng, Benjamin M. Heineike, Christina M. Fitzsimmons, Benjamin L. Oakes, Jiuxin Qu, Neha Prasad, Oren S. Rosenberg, David F. Savage, Hana El-Samad, Jennifer A. Doudna, Tanja Kortemme
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/75a198d8f2ba47a985055e98863b4ffd
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spelling	oai:doaj.org-article:75a198d8f2ba47a985055e98863b4ffd2021-12-02T16:57:51ZControlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs10.1038/s41467-019-09985-22041-1723https://doaj.org/article/75a198d8f2ba47a985055e98863b4ffd2019-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09985-2https://doaj.org/toc/2041-1723Control of CRISPR-Cas9 activity allows for fine-tuning of editing and gene expression. Here the authors use gRNAs modified with RNA aptamers to enable small molecule control in bacterial systems.Kale KundertJames E. LucasKyle E. WattersChristof FellmannAndrew H. NgBenjamin M. HeineikeChristina M. FitzsimmonsBenjamin L. OakesJiuxin QuNeha PrasadOren S. RosenbergDavid F. SavageHana El-SamadJennifer A. DoudnaTanja KortemmeNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Kale Kundert James E. Lucas Kyle E. Watters Christof Fellmann Andrew H. Ng Benjamin M. Heineike Christina M. Fitzsimmons Benjamin L. Oakes Jiuxin Qu Neha Prasad Oren S. Rosenberg David F. Savage Hana El-Samad Jennifer A. Doudna Tanja Kortemme Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
description	Control of CRISPR-Cas9 activity allows for fine-tuning of editing and gene expression. Here the authors use gRNAs modified with RNA aptamers to enable small molecule control in bacterial systems.
format	article
author	Kale Kundert James E. Lucas Kyle E. Watters Christof Fellmann Andrew H. Ng Benjamin M. Heineike Christina M. Fitzsimmons Benjamin L. Oakes Jiuxin Qu Neha Prasad Oren S. Rosenberg David F. Savage Hana El-Samad Jennifer A. Doudna Tanja Kortemme
author_facet	Kale Kundert James E. Lucas Kyle E. Watters Christof Fellmann Andrew H. Ng Benjamin M. Heineike Christina M. Fitzsimmons Benjamin L. Oakes Jiuxin Qu Neha Prasad Oren S. Rosenberg David F. Savage Hana El-Samad Jennifer A. Doudna Tanja Kortemme
author_sort	Kale Kundert
title	Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
title_short	Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
title_full	Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
title_fullStr	Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
title_full_unstemmed	Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
title_sort	controlling crispr-cas9 with ligand-activated and ligand-deactivated sgrnas
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/75a198d8f2ba47a985055e98863b4ffd
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Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs

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