In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye

Abstract Optical coherence tomography (OCT) has become a standard-of-care in retinal imaging. OCT allows non-invasive imaging of the tissue structure but lacks specificity to contrast agents that could be used for in vivo molecular imaging. Photothermal OCT (PT-OCT) is a functional OCT-based techniq...

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Autores principales:	Maryse Lapierre-Landry, Andrew Y. Gordon, John S. Penn, Melissa C. Skala
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/75a620aefc3d4fd68c5208ae345acb28
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spelling	oai:doaj.org-article:75a620aefc3d4fd68c5208ae345acb282021-12-02T15:06:07ZIn vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye10.1038/s41598-017-10050-52045-2322https://doaj.org/article/75a620aefc3d4fd68c5208ae345acb282017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-10050-5https://doaj.org/toc/2045-2322Abstract Optical coherence tomography (OCT) has become a standard-of-care in retinal imaging. OCT allows non-invasive imaging of the tissue structure but lacks specificity to contrast agents that could be used for in vivo molecular imaging. Photothermal OCT (PT-OCT) is a functional OCT-based technique that has been developed to detect absorbers in a sample. We demonstrate in vivo PT-OCT in the eye for the first time on both endogenous (melanin) and exogenous (gold nanorods) absorbers. Pigmented mice and albino mice (n = 6 eyes) were used to isolate the photothermal signal from the melanin in the retina. Pigmented mice with laser-induced choroidal neovascularization lesions (n = 7 eyes) were also imaged after a systemic injection of gold nanorods to observe their passive accumulation in the retina. This experiment demonstrates the feasibility of PT-OCT to image the distribution of both endogenous and exogenous absorbers in the mouse retina.Maryse Lapierre-LandryAndrew Y. GordonJohn S. PennMelissa C. SkalaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maryse Lapierre-Landry Andrew Y. Gordon John S. Penn Melissa C. Skala In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye
description	Abstract Optical coherence tomography (OCT) has become a standard-of-care in retinal imaging. OCT allows non-invasive imaging of the tissue structure but lacks specificity to contrast agents that could be used for in vivo molecular imaging. Photothermal OCT (PT-OCT) is a functional OCT-based technique that has been developed to detect absorbers in a sample. We demonstrate in vivo PT-OCT in the eye for the first time on both endogenous (melanin) and exogenous (gold nanorods) absorbers. Pigmented mice and albino mice (n = 6 eyes) were used to isolate the photothermal signal from the melanin in the retina. Pigmented mice with laser-induced choroidal neovascularization lesions (n = 7 eyes) were also imaged after a systemic injection of gold nanorods to observe their passive accumulation in the retina. This experiment demonstrates the feasibility of PT-OCT to image the distribution of both endogenous and exogenous absorbers in the mouse retina.
format	article
author	Maryse Lapierre-Landry Andrew Y. Gordon John S. Penn Melissa C. Skala
author_facet	Maryse Lapierre-Landry Andrew Y. Gordon John S. Penn Melissa C. Skala
author_sort	Maryse Lapierre-Landry
title	In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye
title_short	In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye
title_full	In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye
title_fullStr	In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye
title_full_unstemmed	In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye
title_sort	in vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/75a620aefc3d4fd68c5208ae345acb28
work_keys_str_mv	AT maryselapierrelandry invivophotothermalopticalcoherencetomographyofendogenousandexogenouscontrastagentsintheeye AT andrewygordon invivophotothermalopticalcoherencetomographyofendogenousandexogenouscontrastagentsintheeye AT johnspenn invivophotothermalopticalcoherencetomographyofendogenousandexogenouscontrastagentsintheeye AT melissacskala invivophotothermalopticalcoherencetomographyofendogenousandexogenouscontrastagentsintheeye
_version_	1718388589508689920

In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye

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