Characterization of novel paternal ncRNAs at the Plagl1 locus, including Hymai, predicted to interact with regulators of active chromatin.
Genomic imprinting is a complex epigenetic mechanism of transcriptional control that utilizes DNA methylation and histone modifications to bring about parent-of-origin specific monoallelic expression in mammals. Genes subject to imprinting are often organised in clusters associated with large non-co...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/75a973f630334971b059ce2ecb05af1d |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:75a973f630334971b059ce2ecb05af1d |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:75a973f630334971b059ce2ecb05af1d2021-11-18T07:15:00ZCharacterization of novel paternal ncRNAs at the Plagl1 locus, including Hymai, predicted to interact with regulators of active chromatin.1932-620310.1371/journal.pone.0038907https://doaj.org/article/75a973f630334971b059ce2ecb05af1d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22723905/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Genomic imprinting is a complex epigenetic mechanism of transcriptional control that utilizes DNA methylation and histone modifications to bring about parent-of-origin specific monoallelic expression in mammals. Genes subject to imprinting are often organised in clusters associated with large non-coding RNAs (ncRNAs), some of which have cis-regulatory functions. Here we have undertaken a detailed allelic expression analysis of an imprinted domain on mouse proximal chromosome 10 comprising the paternally expressed Plagl1 gene. We identified three novel Plagl1 transcripts, only one of which contains protein-coding exons. In addition, we characterised two unspliced ncRNAs, Hymai, the mouse orthologue of HYMAI, and Plagl1it (Plagl1 intronic transcript), a transcript located in intron 5 of Plagl1. Imprinted expression of these novel ncRNAs requires DNMT3L-mediated maternal DNA methylation, which is also indispensable for establishing the correct chromatin profile at the Plagl1 DMR. Significantly, the two ncRNAs are retained in the nucleus, consistent with a potential regulatory function at the imprinted domain. Analysis with catRAPID, a protein-ncRNA association prediction algorithm, suggests that Hymai and Plagl1it RNAs both have potentially high affinity for Trithorax chromatin regulators. The two ncRNAs could therefore help to protect the paternal allele from DNA methylation by attracting Trithorax proteins that mediate H3 lysine-4 methylation.Isabel Iglesias-PlatasAlex Martin-TrujilloDavide CirilloFranck CourtAmy Guillaumet-AdkinsCristina CamprubiDeborah Bourc'hisKenichiro HataRobert FeilGian TartagliaPhilippe ArnaudDavid MonkPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e38907 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Isabel Iglesias-Platas Alex Martin-Trujillo Davide Cirillo Franck Court Amy Guillaumet-Adkins Cristina Camprubi Deborah Bourc'his Kenichiro Hata Robert Feil Gian Tartaglia Philippe Arnaud David Monk Characterization of novel paternal ncRNAs at the Plagl1 locus, including Hymai, predicted to interact with regulators of active chromatin. |
| description |
Genomic imprinting is a complex epigenetic mechanism of transcriptional control that utilizes DNA methylation and histone modifications to bring about parent-of-origin specific monoallelic expression in mammals. Genes subject to imprinting are often organised in clusters associated with large non-coding RNAs (ncRNAs), some of which have cis-regulatory functions. Here we have undertaken a detailed allelic expression analysis of an imprinted domain on mouse proximal chromosome 10 comprising the paternally expressed Plagl1 gene. We identified three novel Plagl1 transcripts, only one of which contains protein-coding exons. In addition, we characterised two unspliced ncRNAs, Hymai, the mouse orthologue of HYMAI, and Plagl1it (Plagl1 intronic transcript), a transcript located in intron 5 of Plagl1. Imprinted expression of these novel ncRNAs requires DNMT3L-mediated maternal DNA methylation, which is also indispensable for establishing the correct chromatin profile at the Plagl1 DMR. Significantly, the two ncRNAs are retained in the nucleus, consistent with a potential regulatory function at the imprinted domain. Analysis with catRAPID, a protein-ncRNA association prediction algorithm, suggests that Hymai and Plagl1it RNAs both have potentially high affinity for Trithorax chromatin regulators. The two ncRNAs could therefore help to protect the paternal allele from DNA methylation by attracting Trithorax proteins that mediate H3 lysine-4 methylation. |
| format |
article |
| author |
Isabel Iglesias-Platas Alex Martin-Trujillo Davide Cirillo Franck Court Amy Guillaumet-Adkins Cristina Camprubi Deborah Bourc'his Kenichiro Hata Robert Feil Gian Tartaglia Philippe Arnaud David Monk |
| author_facet |
Isabel Iglesias-Platas Alex Martin-Trujillo Davide Cirillo Franck Court Amy Guillaumet-Adkins Cristina Camprubi Deborah Bourc'his Kenichiro Hata Robert Feil Gian Tartaglia Philippe Arnaud David Monk |
| author_sort |
Isabel Iglesias-Platas |
| title |
Characterization of novel paternal ncRNAs at the Plagl1 locus, including Hymai, predicted to interact with regulators of active chromatin. |
| title_short |
Characterization of novel paternal ncRNAs at the Plagl1 locus, including Hymai, predicted to interact with regulators of active chromatin. |
| title_full |
Characterization of novel paternal ncRNAs at the Plagl1 locus, including Hymai, predicted to interact with regulators of active chromatin. |
| title_fullStr |
Characterization of novel paternal ncRNAs at the Plagl1 locus, including Hymai, predicted to interact with regulators of active chromatin. |
| title_full_unstemmed |
Characterization of novel paternal ncRNAs at the Plagl1 locus, including Hymai, predicted to interact with regulators of active chromatin. |
| title_sort |
characterization of novel paternal ncrnas at the plagl1 locus, including hymai, predicted to interact with regulators of active chromatin. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/75a973f630334971b059ce2ecb05af1d |
| work_keys_str_mv |
AT isabeliglesiasplatas characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT alexmartintrujillo characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT davidecirillo characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT franckcourt characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT amyguillaumetadkins characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT cristinacamprubi characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT deborahbourchis characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT kenichirohata characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT robertfeil characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT giantartaglia characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT philippearnaud characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin AT davidmonk characterizationofnovelpaternalncrnasattheplagl1locusincludinghymaipredictedtointeractwithregulatorsofactivechromatin |
| _version_ |
1718423734093611008 |