A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line

Abstract The inability to over-express Aquaporin 6 (AQP6) in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane protein at atomic detail using crystallographic approaches. Using an Aquaporin 3-tGFP Reporter (AGR) syst...

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Autores principales:	D. C. Soler, T. Kowatz, A. E. Sloan, T. S. McCormick, K. D. Cooper, R. Stepanyan, A. Engel, A. Vahedi-Faridi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/75ba498787aa4de3baf4ebb95d66607f
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spelling	oai:doaj.org-article:75ba498787aa4de3baf4ebb95d66607f2021-12-02T16:31:54ZA region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line10.1038/s41598-021-93117-82045-2322https://doaj.org/article/75ba498787aa4de3baf4ebb95d66607f2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93117-8https://doaj.org/toc/2045-2322Abstract The inability to over-express Aquaporin 6 (AQP6) in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane protein at atomic detail using crystallographic approaches. Using an Aquaporin 3-tGFP Reporter (AGR) system we have identified a region within loop C of AQP6 that is responsible for severely hampering plasma membrane expression. Serine substitution corroborated that amino acids present within AQP6194–213 of AQP6 loop C contribute to intracellular endoplasmic reticulum (ER) retention. This intracellular retention signal may preclude proper plasma membrane trafficking and severely curtail expression of AQP6 in heterologous expression systems.D. C. SolerT. KowatzA. E. SloanT. S. McCormickK. D. CooperR. StepanyanA. EngelA. Vahedi-FaridiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q D. C. Soler T. Kowatz A. E. Sloan T. S. McCormick K. D. Cooper R. Stepanyan A. Engel A. Vahedi-Faridi A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
description	Abstract The inability to over-express Aquaporin 6 (AQP6) in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane protein at atomic detail using crystallographic approaches. Using an Aquaporin 3-tGFP Reporter (AGR) system we have identified a region within loop C of AQP6 that is responsible for severely hampering plasma membrane expression. Serine substitution corroborated that amino acids present within AQP6194–213 of AQP6 loop C contribute to intracellular endoplasmic reticulum (ER) retention. This intracellular retention signal may preclude proper plasma membrane trafficking and severely curtail expression of AQP6 in heterologous expression systems.
format	article
author	D. C. Soler T. Kowatz A. E. Sloan T. S. McCormick K. D. Cooper R. Stepanyan A. Engel A. Vahedi-Faridi
author_facet	D. C. Soler T. Kowatz A. E. Sloan T. S. McCormick K. D. Cooper R. Stepanyan A. Engel A. Vahedi-Faridi
author_sort	D. C. Soler
title	A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title_short	A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title_full	A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title_fullStr	A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title_full_unstemmed	A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title_sort	region within the third extracellular loop of rat aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/75ba498787aa4de3baf4ebb95d66607f
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A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line

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