The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei
Abstract Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spect...
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Nature Portfolio
2017
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oai:doaj.org-article:75bad0eb60ca4dda904e608ea8efed112021-12-02T11:52:43ZThe Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei10.1038/s41598-017-01038-22045-2322https://doaj.org/article/75bad0eb60ca4dda904e608ea8efed112017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01038-2https://doaj.org/toc/2045-2322Abstract Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Principal component analysis (PCA) of the processed LC-MS data demonstrated the varying chemical composition of the samples. Most of the samples were active against Trypanosoma b. brucei with the highest activity being in the samples from Southern Nigeria. The more active samples were fractionated in order to isolate the component(s) responsible for their activity using medium pressure liquid chromatography (MPLC). Three xanthones, 1,3,7-trihydroxy-2,8-di-(3-methylbut-2-enyl)xanthone, 1,3,7-trihydroxy-4,8-di-(3-methylbut-2-enyl)xanthone a previously undescribed xanthone and three triterpenes: ambonic acid, mangiferonic acid and a mixture of α-amyrin with mangiferonic acid (1:3) were isolated and characterised by NMR and LC-MS. These compounds all displayed strong inhibitory activity against T.b. brucei but none of them had higher activity than the crude extracts. Partial least squares (PLS) modelling of the anti-trypanosomal activity of the sample extracts using the LC-MS data indicated that high activity in the extracts, as judged from LCMS2 data, could be correlated to denticulatain isomers in the extracts.Ruwida OmarJohn O. IgoliTong ZhangAlexander I. GrayGodwin U. EbilomaCarol J. ClementsJames FearnleyRuAngeli Edrada EbelTim PagetHarry P. de KoningDavid G. WatsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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EN |
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Medicine R Science Q |
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Medicine R Science Q Ruwida Omar John O. Igoli Tong Zhang Alexander I. Gray Godwin U. Ebiloma Carol J. Clements James Fearnley RuAngeli Edrada Ebel Tim Paget Harry P. de Koning David G. Watson The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
| description |
Abstract Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Principal component analysis (PCA) of the processed LC-MS data demonstrated the varying chemical composition of the samples. Most of the samples were active against Trypanosoma b. brucei with the highest activity being in the samples from Southern Nigeria. The more active samples were fractionated in order to isolate the component(s) responsible for their activity using medium pressure liquid chromatography (MPLC). Three xanthones, 1,3,7-trihydroxy-2,8-di-(3-methylbut-2-enyl)xanthone, 1,3,7-trihydroxy-4,8-di-(3-methylbut-2-enyl)xanthone a previously undescribed xanthone and three triterpenes: ambonic acid, mangiferonic acid and a mixture of α-amyrin with mangiferonic acid (1:3) were isolated and characterised by NMR and LC-MS. These compounds all displayed strong inhibitory activity against T.b. brucei but none of them had higher activity than the crude extracts. Partial least squares (PLS) modelling of the anti-trypanosomal activity of the sample extracts using the LC-MS data indicated that high activity in the extracts, as judged from LCMS2 data, could be correlated to denticulatain isomers in the extracts. |
| format |
article |
| author |
Ruwida Omar John O. Igoli Tong Zhang Alexander I. Gray Godwin U. Ebiloma Carol J. Clements James Fearnley RuAngeli Edrada Ebel Tim Paget Harry P. de Koning David G. Watson |
| author_facet |
Ruwida Omar John O. Igoli Tong Zhang Alexander I. Gray Godwin U. Ebiloma Carol J. Clements James Fearnley RuAngeli Edrada Ebel Tim Paget Harry P. de Koning David G. Watson |
| author_sort |
Ruwida Omar |
| title |
The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
| title_short |
The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
| title_full |
The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
| title_fullStr |
The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
| title_full_unstemmed |
The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
| title_sort |
chemical characterization of nigerian propolis samples and their activity against trypanosoma brucei |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/75bad0eb60ca4dda904e608ea8efed11 |
| work_keys_str_mv |
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