CONTENTS OF THYROID HORMONES, CYTOKINES AND α2-MACROGLOBULIN IN BLOOD SERA AND IN CULTURE SUPERNATES OF BLOOD CELLS FROM THE GRAVES DISEASE PATIENTS

We had investigated levels of TTG, T4, TNFα, IL-6, IFNγ, and α2-MG in blood serum and supernates of short-term blood cultures in the patients with verified Graves disease before treatment and after reaching of euthyroid status, as compared with healthy controls. We have revealed that initial blood c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	V. N. Zorina, T. P. Maklakova, T. T. Sheppel, O. N. Boyko, R. M. Zorina, N. A. Zorin
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2015
Materias:	graves disease cytokines α2-macroglobulin blood cells cultures supernatant mitogen stimulation thiamazole Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/75c1ad349a154589b3fc45032abba56d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:75c1ad349a154589b3fc45032abba56d
record_format	dspace
spelling	oai:doaj.org-article:75c1ad349a154589b3fc45032abba56d2021-11-18T08:03:44ZCONTENTS OF THYROID HORMONES, CYTOKINES AND α2-MACROGLOBULIN IN BLOOD SERA AND IN CULTURE SUPERNATES OF BLOOD CELLS FROM THE GRAVES DISEASE PATIENTS1563-06252313-741X10.15789/1563-0625-2015-1-53-58https://doaj.org/article/75c1ad349a154589b3fc45032abba56d2015-03-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/808https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XWe had investigated levels of TTG, T4, TNFα, IL-6, IFNγ, and α2-MG in blood serum and supernates of short-term blood cultures in the patients with verified Graves disease before treatment and after reaching of euthyroid status, as compared with healthy controls. We have revealed that initial blood concentrations of free Т4 in the patients were increased, along with decrease in TSH, higher IL-6, IFNγ levels, as well as concentrations of α2-MG which participates in cytokine transport and synthesis. Thiamazole treatment normalized the hormonal profile and reduced blood levels of IL-6, IFNγ and α2-MG, however, without complete normalization, along with increase of serum TNFα contents. It was shown, that the patients before treatment had decreased in vitro response of cells to the mitogenic stimulation as shown by decreased induction of TNFα and IFNγ production, along with, increased spontaneous IFNγ levels. When reaching euthyroid state after Thiamazole administration, we observed an increased spontaneous IFNγ synthesis, decreased IL-6 production in resting cultures. In mitogen-stimulated cell cultures from the treated patients, IFNγ contents became normal, however, TNFα secretion remained lower than in controls. The α2-MG levels in supernates were stable and significantly lower, than in serum. We may presume that thyrotoxicosis treatment with Thiamazole causes stabilization of the endocrine state, however, being not sufficient for normalized production of cytokines, as well as α2-MG, with its regulatory and transporter functions, thus promoting recurrence of disease and reactivation of autoimmune events.V. N. ZorinaT. P. MaklakovaT. T. SheppelO. N. BoykoR. M. ZorinaN. A. ZorinSPb RAACIarticlegraves diseasecytokinesα2-macroglobulinblood cells culturessupernatantmitogen stimulationthiamazoleImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 17, Iss 1, Pp 53-58 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	graves disease cytokines α2-macroglobulin blood cells cultures supernatant mitogen stimulation thiamazole Immunologic diseases. Allergy RC581-607
spellingShingle	graves disease cytokines α2-macroglobulin blood cells cultures supernatant mitogen stimulation thiamazole Immunologic diseases. Allergy RC581-607 V. N. Zorina T. P. Maklakova T. T. Sheppel O. N. Boyko R. M. Zorina N. A. Zorin CONTENTS OF THYROID HORMONES, CYTOKINES AND α2-MACROGLOBULIN IN BLOOD SERA AND IN CULTURE SUPERNATES OF BLOOD CELLS FROM THE GRAVES DISEASE PATIENTS
description	We had investigated levels of TTG, T4, TNFα, IL-6, IFNγ, and α2-MG in blood serum and supernates of short-term blood cultures in the patients with verified Graves disease before treatment and after reaching of euthyroid status, as compared with healthy controls. We have revealed that initial blood concentrations of free Т4 in the patients were increased, along with decrease in TSH, higher IL-6, IFNγ levels, as well as concentrations of α2-MG which participates in cytokine transport and synthesis. Thiamazole treatment normalized the hormonal profile and reduced blood levels of IL-6, IFNγ and α2-MG, however, without complete normalization, along with increase of serum TNFα contents. It was shown, that the patients before treatment had decreased in vitro response of cells to the mitogenic stimulation as shown by decreased induction of TNFα and IFNγ production, along with, increased spontaneous IFNγ levels. When reaching euthyroid state after Thiamazole administration, we observed an increased spontaneous IFNγ synthesis, decreased IL-6 production in resting cultures. In mitogen-stimulated cell cultures from the treated patients, IFNγ contents became normal, however, TNFα secretion remained lower than in controls. The α2-MG levels in supernates were stable and significantly lower, than in serum. We may presume that thyrotoxicosis treatment with Thiamazole causes stabilization of the endocrine state, however, being not sufficient for normalized production of cytokines, as well as α2-MG, with its regulatory and transporter functions, thus promoting recurrence of disease and reactivation of autoimmune events.
format	article
author	V. N. Zorina T. P. Maklakova T. T. Sheppel O. N. Boyko R. M. Zorina N. A. Zorin
author_facet	V. N. Zorina T. P. Maklakova T. T. Sheppel O. N. Boyko R. M. Zorina N. A. Zorin
author_sort	V. N. Zorina
title	CONTENTS OF THYROID HORMONES, CYTOKINES AND α2-MACROGLOBULIN IN BLOOD SERA AND IN CULTURE SUPERNATES OF BLOOD CELLS FROM THE GRAVES DISEASE PATIENTS
title_short	CONTENTS OF THYROID HORMONES, CYTOKINES AND α2-MACROGLOBULIN IN BLOOD SERA AND IN CULTURE SUPERNATES OF BLOOD CELLS FROM THE GRAVES DISEASE PATIENTS
title_full	CONTENTS OF THYROID HORMONES, CYTOKINES AND α2-MACROGLOBULIN IN BLOOD SERA AND IN CULTURE SUPERNATES OF BLOOD CELLS FROM THE GRAVES DISEASE PATIENTS
title_fullStr	CONTENTS OF THYROID HORMONES, CYTOKINES AND α2-MACROGLOBULIN IN BLOOD SERA AND IN CULTURE SUPERNATES OF BLOOD CELLS FROM THE GRAVES DISEASE PATIENTS
title_full_unstemmed	CONTENTS OF THYROID HORMONES, CYTOKINES AND α2-MACROGLOBULIN IN BLOOD SERA AND IN CULTURE SUPERNATES OF BLOOD CELLS FROM THE GRAVES DISEASE PATIENTS
title_sort	contents of thyroid hormones, cytokines and α2-macroglobulin in blood sera and in culture supernates of blood cells from the graves disease patients
publisher	SPb RAACI
publishDate	2015
url	https://doaj.org/article/75c1ad349a154589b3fc45032abba56d
work_keys_str_mv	AT vnzorina contentsofthyroidhormonescytokinesanda2macroglobulininbloodseraandinculturesupernatesofbloodcellsfromthegravesdiseasepatients AT tpmaklakova contentsofthyroidhormonescytokinesanda2macroglobulininbloodseraandinculturesupernatesofbloodcellsfromthegravesdiseasepatients AT ttsheppel contentsofthyroidhormonescytokinesanda2macroglobulininbloodseraandinculturesupernatesofbloodcellsfromthegravesdiseasepatients AT onboyko contentsofthyroidhormonescytokinesanda2macroglobulininbloodseraandinculturesupernatesofbloodcellsfromthegravesdiseasepatients AT rmzorina contentsofthyroidhormonescytokinesanda2macroglobulininbloodseraandinculturesupernatesofbloodcellsfromthegravesdiseasepatients AT nazorin contentsofthyroidhormonescytokinesanda2macroglobulininbloodseraandinculturesupernatesofbloodcellsfromthegravesdiseasepatients
_version_	1718422427243905024

CONTENTS OF THYROID HORMONES, CYTOKINES AND α2-MACROGLOBULIN IN BLOOD SERA AND IN CULTURE SUPERNATES OF BLOOD CELLS FROM THE GRAVES DISEASE PATIENTS

Ejemplares similares