Specific inhibition of FGF5-induced cell proliferation by RNA aptamers

Abstract Fibroblast growth factor 5 (FGF5) is a crucial regulator of hair growth and an oncogenic factor in several human cancers. To generate FGF5 inhibitors, we performed Systematic Evolution of Ligands by EXponential enrichment and obtained novel RNA aptamers that have high affinity to human FGF5...

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Autores principales:	Ryo Amano, Masato Namekata, Masataka Horiuchi, Minami Saso, Takuya Yanagisawa, Yoichiro Tanaka, Farhana Ishrat Ghani, Masakuni Yamamoto, Taiichi Sakamoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/75c971d601c64b4c9394e84857b8cf47
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spelling	oai:doaj.org-article:75c971d601c64b4c9394e84857b8cf472021-12-02T10:44:14ZSpecific inhibition of FGF5-induced cell proliferation by RNA aptamers10.1038/s41598-021-82350-w2045-2322https://doaj.org/article/75c971d601c64b4c9394e84857b8cf472021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82350-whttps://doaj.org/toc/2045-2322Abstract Fibroblast growth factor 5 (FGF5) is a crucial regulator of hair growth and an oncogenic factor in several human cancers. To generate FGF5 inhibitors, we performed Systematic Evolution of Ligands by EXponential enrichment and obtained novel RNA aptamers that have high affinity to human FGF5. These aptamers inhibited FGF5-induced cell proliferation, but did not inhibit FGF2-induced cell proliferation. Surface plasmon resonance demonstrated that one of the aptamers, F5f1, binds to FGF5 tightly (K d = 0.7 ± 0.2 nM), but did not fully to FGF1, FGF2, FGF4, FGF6, or FGFR1. Based on sequence and secondary structure similarities of the aptamers, we generated the truncated aptamer, F5f1_56, which has higher affinity (K d = 0.118 ± 0.003 nM) than the original F5f1. Since the aptamers have high affinity and specificity to FGF5 and inhibit FGF5-induced cell proliferation, they may be candidates for therapeutic use with FGF5-related diseases or hair disorders.Ryo AmanoMasato NamekataMasataka HoriuchiMinami SasoTakuya YanagisawaYoichiro TanakaFarhana Ishrat GhaniMasakuni YamamotoTaiichi SakamotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryo Amano Masato Namekata Masataka Horiuchi Minami Saso Takuya Yanagisawa Yoichiro Tanaka Farhana Ishrat Ghani Masakuni Yamamoto Taiichi Sakamoto Specific inhibition of FGF5-induced cell proliferation by RNA aptamers
description	Abstract Fibroblast growth factor 5 (FGF5) is a crucial regulator of hair growth and an oncogenic factor in several human cancers. To generate FGF5 inhibitors, we performed Systematic Evolution of Ligands by EXponential enrichment and obtained novel RNA aptamers that have high affinity to human FGF5. These aptamers inhibited FGF5-induced cell proliferation, but did not inhibit FGF2-induced cell proliferation. Surface plasmon resonance demonstrated that one of the aptamers, F5f1, binds to FGF5 tightly (K d = 0.7 ± 0.2 nM), but did not fully to FGF1, FGF2, FGF4, FGF6, or FGFR1. Based on sequence and secondary structure similarities of the aptamers, we generated the truncated aptamer, F5f1_56, which has higher affinity (K d = 0.118 ± 0.003 nM) than the original F5f1. Since the aptamers have high affinity and specificity to FGF5 and inhibit FGF5-induced cell proliferation, they may be candidates for therapeutic use with FGF5-related diseases or hair disorders.
format	article
author	Ryo Amano Masato Namekata Masataka Horiuchi Minami Saso Takuya Yanagisawa Yoichiro Tanaka Farhana Ishrat Ghani Masakuni Yamamoto Taiichi Sakamoto
author_facet	Ryo Amano Masato Namekata Masataka Horiuchi Minami Saso Takuya Yanagisawa Yoichiro Tanaka Farhana Ishrat Ghani Masakuni Yamamoto Taiichi Sakamoto
author_sort	Ryo Amano
title	Specific inhibition of FGF5-induced cell proliferation by RNA aptamers
title_short	Specific inhibition of FGF5-induced cell proliferation by RNA aptamers
title_full	Specific inhibition of FGF5-induced cell proliferation by RNA aptamers
title_fullStr	Specific inhibition of FGF5-induced cell proliferation by RNA aptamers
title_full_unstemmed	Specific inhibition of FGF5-induced cell proliferation by RNA aptamers
title_sort	specific inhibition of fgf5-induced cell proliferation by rna aptamers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/75c971d601c64b4c9394e84857b8cf47
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Specific inhibition of FGF5-induced cell proliferation by RNA aptamers

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