Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report

Summary Anamnesis: a six-year-old milking Holstein cow (Bos taurus taurus) was diagnosed with stage IV Johne´s Disease (JD). Clinical and Laboratory findings: the cow suffered intermittent diarrhea during 6 months with no response to antibiotic treatment. Consequently, the cow was subjected to eut...

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Autores principales:	René Ramírez-Garcia, Juan G Maldonado-Estrada
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2013
Materias:	Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/75cd7a14538844d0a584f5368f524dde
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spelling	oai:doaj.org-article:75cd7a14538844d0a584f5368f524dde2021-12-01T14:57:06ZDetection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report2256-2958https://doaj.org/article/75cd7a14538844d0a584f5368f524dde2013-09-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/324832https://doaj.org/toc/2256-2958Summary Anamnesis: a six-year-old milking Holstein cow (Bos taurus taurus) was diagnosed with stage IV Johne´s Disease (JD). Clinical and Laboratory findings: the cow suffered intermittent diarrhea during 6 months with no response to antibiotic treatment. Consequently, the cow was subjected to euthanasia. Treatment approach: antemortem milk and peripheral blood samples and postmortem colon, mediastinic, mesenteric lymph nodes, and spleen samples were processed for macrophages isolation. Total DNA was extracted from macrophages and used to diagnose IS900 of Mycobacterium avium paratuberculosis (MAP) through real time PCR. The MAP IS900 segment was successfully amplified from cells of all samples, indicating that these cells were MAP-infected macrophages. Conclusion: macrophages of cows suffering from JD can be used for amplification of the MAP IS900 segment. Key words: intracellular bacteria, real time PCR, resident macrophages, susceptible host. Resumen Anamnesis: una vaca Holstein de 6 años de edad (Bos taurus taurus) presentó sintomatología de la fase IV de la enfermedad de Johne. Hallazgos clínicos y de laboratorio: la vaca presentó diarrea intermitente durante 6 meses sin respuesta al tratamiento con antibióticos. En consecuencia, la vaca fue sometida a eutanasia. Esquema de tratamiento: muestras de leche y de sangre periférica se tomaron ante-mortem; muestras de la mucosa del intestino, bazo y linfonodos mediastínico y mesentérico, se tomaron post-mortem, todas para aislamiento de macrófagos. El ADN total de los macrófagos fue usado para la amplificación del segmento IS900 de Mycobacterium avium paratuberculosis (MAP) por PCR en tiempo real. Conclusión: los macrófagos de vacas con la enfermedad de Johne pueden ser usados para la amplificación del segmento IS900 de MAP. Palabras clave: bacteria intracelular, hospedero susceptible, macrófagos residentes, PCR en tiempo real. Resumo Anamnese: uma vaca Holstein com 6 anos de idade (Bos taurus taurus) apresentou os sintomas da fase IV da doença de Johne. Achados clínicos e laboratoriais: a vaca teve diarreia intermitente por seis meses sem resposta ao tratamento com antibióticos. Por conseguinte, a vaca foi submetida à eutanásia. Abordagem de tratamento: amostras de leite e de sangue periférico foram retiradas ante-mortem, enquanto as amostras da mucosa intestinal, baço e linfonodos mesentéricos e mediastinais foram tomadas todas post-mortem para o isolamento de macrófagos. O ADN total de macrófagos foi utilizado para amplificação do segmento IS900 do Mycobacterium avium paratuberculosis (MAP) por PCR em tempo real. Conclusão: macrófagos isolados a partir de vacas com doença de Johne podem ser utilizados para a amplificação da segmento IS900 do MAP. Palavras chave: bactérias intracelulares, hospedeiro suscetível, macrófagos residentes, PCR em tempo real.René Ramírez-GarciaJuan G Maldonado-EstradaUniversidad de AntioquiaarticleAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 26, Iss 3, Pp 219-225 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Animal culture SF1-1100
spellingShingle	Animal culture SF1-1100 René Ramírez-Garcia Juan G Maldonado-Estrada Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report
description	Summary Anamnesis: a six-year-old milking Holstein cow (Bos taurus taurus) was diagnosed with stage IV Johne´s Disease (JD). Clinical and Laboratory findings: the cow suffered intermittent diarrhea during 6 months with no response to antibiotic treatment. Consequently, the cow was subjected to euthanasia. Treatment approach: antemortem milk and peripheral blood samples and postmortem colon, mediastinic, mesenteric lymph nodes, and spleen samples were processed for macrophages isolation. Total DNA was extracted from macrophages and used to diagnose IS900 of Mycobacterium avium paratuberculosis (MAP) through real time PCR. The MAP IS900 segment was successfully amplified from cells of all samples, indicating that these cells were MAP-infected macrophages. Conclusion: macrophages of cows suffering from JD can be used for amplification of the MAP IS900 segment. Key words: intracellular bacteria, real time PCR, resident macrophages, susceptible host. Resumen Anamnesis: una vaca Holstein de 6 años de edad (Bos taurus taurus) presentó sintomatología de la fase IV de la enfermedad de Johne. Hallazgos clínicos y de laboratorio: la vaca presentó diarrea intermitente durante 6 meses sin respuesta al tratamiento con antibióticos. En consecuencia, la vaca fue sometida a eutanasia. Esquema de tratamiento: muestras de leche y de sangre periférica se tomaron ante-mortem; muestras de la mucosa del intestino, bazo y linfonodos mediastínico y mesentérico, se tomaron post-mortem, todas para aislamiento de macrófagos. El ADN total de los macrófagos fue usado para la amplificación del segmento IS900 de Mycobacterium avium paratuberculosis (MAP) por PCR en tiempo real. Conclusión: los macrófagos de vacas con la enfermedad de Johne pueden ser usados para la amplificación del segmento IS900 de MAP. Palabras clave: bacteria intracelular, hospedero susceptible, macrófagos residentes, PCR en tiempo real. Resumo Anamnese: uma vaca Holstein com 6 anos de idade (Bos taurus taurus) apresentou os sintomas da fase IV da doença de Johne. Achados clínicos e laboratoriais: a vaca teve diarreia intermitente por seis meses sem resposta ao tratamento com antibióticos. Por conseguinte, a vaca foi submetida à eutanásia. Abordagem de tratamento: amostras de leite e de sangue periférico foram retiradas ante-mortem, enquanto as amostras da mucosa intestinal, baço e linfonodos mesentéricos e mediastinais foram tomadas todas post-mortem para o isolamento de macrófagos. O ADN total de macrófagos foi utilizado para amplificação do segmento IS900 do Mycobacterium avium paratuberculosis (MAP) por PCR em tempo real. Conclusão: macrófagos isolados a partir de vacas com doença de Johne podem ser utilizados para a amplificação da segmento IS900 do MAP. Palavras chave: bactérias intracelulares, hospedeiro suscetível, macrófagos residentes, PCR em tempo real.
format	article
author	René Ramírez-Garcia Juan G Maldonado-Estrada
author_facet	René Ramírez-Garcia Juan G Maldonado-Estrada
author_sort	René Ramírez-Garcia
title	Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report
title_short	Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report
title_full	Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report
title_fullStr	Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report
title_full_unstemmed	Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report
title_sort	detection of macrophages infected with mycobacterium avium subspecies paratuberculosis in a cow with clinical stage iv of johne's disease. a case report
publisher	Universidad de Antioquia
publishDate	2013
url	https://doaj.org/article/75cd7a14538844d0a584f5368f524dde
work_keys_str_mv	AT reneramirezgarcia detectionofmacrophagesinfectedwithmycobacteriumaviumsubspeciesparatuberculosisinacowwithclinicalstageivofjohnesdiseaseacasereport AT juangmaldonadoestrada detectionofmacrophagesinfectedwithmycobacteriumaviumsubspeciesparatuberculosisinacowwithclinicalstageivofjohnesdiseaseacasereport
_version_	1718404877994950656

Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report

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