New York City House Mice (<named-content content-type="genus-species">Mus musculus</named-content>) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants

ABSTRACT House mice (Mus musculus) thrive in large urban centers worldwide. Nonetheless, little is known about the role that they may play in contributing to environmental contamination with potentially pathogenic bacteria. Here, we describe the fecal microbiome of house mice with emphasis on detect...

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Autores principales:	Simon H. Williams, Xiaoyu Che, Ashley Paulick, Cheng Guo, Bohyun Lee, Dorothy Muller, Anne-Catrin Uhlemann, Franklin D. Lowy, Robert M. Corrigan, W. Ian Lipkin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	antimicrobial resistance bacteriome mice New York City Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/75dae800e6784ebbbae1045c4bfba38b
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spelling	oai:doaj.org-article:75dae800e6784ebbbae1045c4bfba38b2021-11-15T15:53:27ZNew York City House Mice (<named-content content-type="genus-species">Mus musculus</named-content>) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants10.1128/mBio.00624-182150-7511https://doaj.org/article/75dae800e6784ebbbae1045c4bfba38b2018-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00624-18https://doaj.org/toc/2150-7511ABSTRACT House mice (Mus musculus) thrive in large urban centers worldwide. Nonetheless, little is known about the role that they may play in contributing to environmental contamination with potentially pathogenic bacteria. Here, we describe the fecal microbiome of house mice with emphasis on detection of pathogenic bacteria and antimicrobial resistance genes by molecular methods. Four hundred sixteen mice were collected from predominantly residential buildings in seven sites across New York City over a period of 13 months. 16S rRNA sequencing identified Bacteroidetes as dominant and revealed high levels of Proteobacteria. A targeted PCR screen of 11 bacteria, as indicated by 16S rRNA analyses, found that mice are carriers of several gastrointestinal disease-causing agents, including Shigella, Salmonella, Clostridium difficile, and diarrheagenic Escherichia coli. Furthermore, genes mediating antimicrobial resistance to fluoroquinolones (qnrB) and β-lactam drugs (blaSHV and blaACT/MIR) were widely distributed. Culture and molecular strain typing of C. difficile revealed that mice harbor ribotypes associated with human disease, and screening of kidney samples demonstrated genetic evidence of pathogenic Leptospira species. In concert, these findings support the need for further research into the role of house mice as potential reservoirs for human pathogens and antimicrobial resistance in the built environment. IMPORTANCE Mice are commensal pests often found in close proximity to humans, especially in urban centers. We surveyed mice from seven sites across New York City and found multiple pathogenic bacteria associated with febrile and gastrointestinal disease as well as an array of antimicrobial resistance genes.Simon H. WilliamsXiaoyu CheAshley PaulickCheng GuoBohyun LeeDorothy MullerAnne-Catrin UhlemannFranklin D. LowyRobert M. CorriganW. Ian LipkinAmerican Society for Microbiologyarticleantimicrobial resistancebacteriomemiceNew York CityMicrobiologyQR1-502ENmBio, Vol 9, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial resistance bacteriome mice New York City Microbiology QR1-502
spellingShingle	antimicrobial resistance bacteriome mice New York City Microbiology QR1-502 Simon H. Williams Xiaoyu Che Ashley Paulick Cheng Guo Bohyun Lee Dorothy Muller Anne-Catrin Uhlemann Franklin D. Lowy Robert M. Corrigan W. Ian Lipkin New York City House Mice (<named-content content-type="genus-species">Mus musculus</named-content>) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants
description	ABSTRACT House mice (Mus musculus) thrive in large urban centers worldwide. Nonetheless, little is known about the role that they may play in contributing to environmental contamination with potentially pathogenic bacteria. Here, we describe the fecal microbiome of house mice with emphasis on detection of pathogenic bacteria and antimicrobial resistance genes by molecular methods. Four hundred sixteen mice were collected from predominantly residential buildings in seven sites across New York City over a period of 13 months. 16S rRNA sequencing identified Bacteroidetes as dominant and revealed high levels of Proteobacteria. A targeted PCR screen of 11 bacteria, as indicated by 16S rRNA analyses, found that mice are carriers of several gastrointestinal disease-causing agents, including Shigella, Salmonella, Clostridium difficile, and diarrheagenic Escherichia coli. Furthermore, genes mediating antimicrobial resistance to fluoroquinolones (qnrB) and β-lactam drugs (blaSHV and blaACT/MIR) were widely distributed. Culture and molecular strain typing of C. difficile revealed that mice harbor ribotypes associated with human disease, and screening of kidney samples demonstrated genetic evidence of pathogenic Leptospira species. In concert, these findings support the need for further research into the role of house mice as potential reservoirs for human pathogens and antimicrobial resistance in the built environment. IMPORTANCE Mice are commensal pests often found in close proximity to humans, especially in urban centers. We surveyed mice from seven sites across New York City and found multiple pathogenic bacteria associated with febrile and gastrointestinal disease as well as an array of antimicrobial resistance genes.
format	article
author	Simon H. Williams Xiaoyu Che Ashley Paulick Cheng Guo Bohyun Lee Dorothy Muller Anne-Catrin Uhlemann Franklin D. Lowy Robert M. Corrigan W. Ian Lipkin
author_facet	Simon H. Williams Xiaoyu Che Ashley Paulick Cheng Guo Bohyun Lee Dorothy Muller Anne-Catrin Uhlemann Franklin D. Lowy Robert M. Corrigan W. Ian Lipkin
author_sort	Simon H. Williams
title	New York City House Mice (<named-content content-type="genus-species">Mus musculus</named-content>) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants
title_short	New York City House Mice (<named-content content-type="genus-species">Mus musculus</named-content>) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants
title_full	New York City House Mice (<named-content content-type="genus-species">Mus musculus</named-content>) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants
title_fullStr	New York City House Mice (<named-content content-type="genus-species">Mus musculus</named-content>) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants
title_full_unstemmed	New York City House Mice (<named-content content-type="genus-species">Mus musculus</named-content>) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants
title_sort	new york city house mice (<named-content content-type="genus-species">mus musculus</named-content>) as potential reservoirs for pathogenic bacteria and antimicrobial resistance determinants
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/75dae800e6784ebbbae1045c4bfba38b
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New York City House Mice (<named-content content-type="genus-species">Mus musculus</named-content>) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants

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