Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood.

<h4>Background</h4>Point-of-care (PoC) testing of platelet count (PLT) provides real-time data for rapid decision making. The goal of this study is to evaluate the accuracy and precision of platelet counting using a new microvolume (8 μL), absolute counting, 1.5 kg cytometry-based blood...

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Autores principales:	William M Dickerson, Rebecca Yu, Helena U Westergren, Jonathan Paraskos, Philipp Schatz, Anna Tigerstrom, Anna Ekman, José Sánchez, Jamie Cheng, Lillian Li, Eugene Y Chan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/75e1cb5715034652b85e7af2b9e48fdd
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spelling	oai:doaj.org-article:75e1cb5715034652b85e7af2b9e48fdd2021-12-02T20:19:32ZPoint-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood.1932-620310.1371/journal.pone.0256423https://doaj.org/article/75e1cb5715034652b85e7af2b9e48fdd2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256423https://doaj.org/toc/1932-6203<h4>Background</h4>Point-of-care (PoC) testing of platelet count (PLT) provides real-time data for rapid decision making. The goal of this study is to evaluate the accuracy and precision of platelet counting using a new microvolume (8 μL), absolute counting, 1.5 kg cytometry-based blood analyzer, the rHEALTH ONE (rHEALTH) in comparison with the International Society of Laboratory Hematology (ISLH) platelet method, which uses a cytometer and an impedance analyzer.<h4>Methods</h4>Inclusion eligibility were healthy adults (M/F) ages 18-80 for donation of fingerprick and venous blood samples. Samples were from a random N = 31 volunteers from a single U.S. site. Samples were serially diluted to test thrombocytopenic ranges. Interfering substances and conditions were tested, including RBC fragments, platelet fragments, cholesterol, triglycerides, lipids, anti-platelet antibodies, and temperature.<h4>Results</h4>The concordance between the rHEALTH and ISLH methods had a slope = 1.030 and R2 = 0.9684. The rHEALTH method showed a correlation between capillary and venous blood samples (slope = 0.9514 and R2 = 0.9684). Certain interferents changed platelet recovery: RBC fragments and anti-platelet antibodies with the ISLH method; platelet fragments and anti-platelet antibodies on the rHEALTH; and RBC fragments, platelets fragments, triglycerides and LDL on the clinical impedance analyzer. The rHEALTH's precision ranged from 3.1-8.0%, and the ISLH from 1.0-10.5%.<h4>Conclusions</h4>The rHEALTH method provides similar results with the reference method and good correlation between adult capillary and venous blood samples. This demonstrates the ability of the rHEALTH to provide point-of-care assessment of normal and thrombocytopenic platelet counts from fingerprick blood with high precision and limited interferences.William M DickersonRebecca YuHelena U WestergrenJonathan ParaskosPhilipp SchatzAnna TigerstromAnna EkmanJosé SánchezJamie ChengLillian LiEugene Y ChanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256423 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q William M Dickerson Rebecca Yu Helena U Westergren Jonathan Paraskos Philipp Schatz Anna Tigerstrom Anna Ekman José Sánchez Jamie Cheng Lillian Li Eugene Y Chan Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood.
description	<h4>Background</h4>Point-of-care (PoC) testing of platelet count (PLT) provides real-time data for rapid decision making. The goal of this study is to evaluate the accuracy and precision of platelet counting using a new microvolume (8 μL), absolute counting, 1.5 kg cytometry-based blood analyzer, the rHEALTH ONE (rHEALTH) in comparison with the International Society of Laboratory Hematology (ISLH) platelet method, which uses a cytometer and an impedance analyzer.<h4>Methods</h4>Inclusion eligibility were healthy adults (M/F) ages 18-80 for donation of fingerprick and venous blood samples. Samples were from a random N = 31 volunteers from a single U.S. site. Samples were serially diluted to test thrombocytopenic ranges. Interfering substances and conditions were tested, including RBC fragments, platelet fragments, cholesterol, triglycerides, lipids, anti-platelet antibodies, and temperature.<h4>Results</h4>The concordance between the rHEALTH and ISLH methods had a slope = 1.030 and R2 = 0.9684. The rHEALTH method showed a correlation between capillary and venous blood samples (slope = 0.9514 and R2 = 0.9684). Certain interferents changed platelet recovery: RBC fragments and anti-platelet antibodies with the ISLH method; platelet fragments and anti-platelet antibodies on the rHEALTH; and RBC fragments, platelets fragments, triglycerides and LDL on the clinical impedance analyzer. The rHEALTH's precision ranged from 3.1-8.0%, and the ISLH from 1.0-10.5%.<h4>Conclusions</h4>The rHEALTH method provides similar results with the reference method and good correlation between adult capillary and venous blood samples. This demonstrates the ability of the rHEALTH to provide point-of-care assessment of normal and thrombocytopenic platelet counts from fingerprick blood with high precision and limited interferences.
format	article
author	William M Dickerson Rebecca Yu Helena U Westergren Jonathan Paraskos Philipp Schatz Anna Tigerstrom Anna Ekman José Sánchez Jamie Cheng Lillian Li Eugene Y Chan
author_facet	William M Dickerson Rebecca Yu Helena U Westergren Jonathan Paraskos Philipp Schatz Anna Tigerstrom Anna Ekman José Sánchez Jamie Cheng Lillian Li Eugene Y Chan
author_sort	William M Dickerson
title	Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood.
title_short	Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood.
title_full	Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood.
title_fullStr	Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood.
title_full_unstemmed	Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood.
title_sort	point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/75e1cb5715034652b85e7af2b9e48fdd
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Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood.

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