A prelude to the proximity interaction mapping of CXXC5

Abstract CXXC5 is a member of the zinc-finger CXXC family proteins that interact with unmodified CpG dinucleotides through a conserved ZF-CXXC domain. CXXC5 is involved in the modulation of gene expressions that lead to alterations in diverse cellular events. However, the underlying mechanism of CXX...

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Autores principales:	Gamze Ayaz, Gizem Turan, Çağla Ece Olgun, Gizem Kars, Burcu Karakaya, Kerim Yavuz, Öykü Deniz Demiralay, Tolga Can, Mesut Muyan, Pelin Yaşar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/75e2878109d14e88b1641504d87a2085
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spelling	oai:doaj.org-article:75e2878109d14e88b1641504d87a20852021-12-02T16:38:49ZA prelude to the proximity interaction mapping of CXXC510.1038/s41598-021-97060-62045-2322https://doaj.org/article/75e2878109d14e88b1641504d87a20852021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97060-6https://doaj.org/toc/2045-2322Abstract CXXC5 is a member of the zinc-finger CXXC family proteins that interact with unmodified CpG dinucleotides through a conserved ZF-CXXC domain. CXXC5 is involved in the modulation of gene expressions that lead to alterations in diverse cellular events. However, the underlying mechanism of CXXC5-modulated gene expressions remains unclear. Proteins perform their functions in a network of proteins whose identities and amounts change spatiotemporally in response to various stimuli in a lineage-specific manner. Since CXXC5 lacks an intrinsic transcription regulatory function or enzymatic activity but is a DNA binder, CXXC5 by interacting with proteins could act as a scaffold to establish a chromatin state restrictive or permissive for transcription. To initially address this, we utilized the proximity-dependent biotinylation approach. Proximity interaction partners of CXXC5 include DNA and chromatin modifiers, transcription factors/co-regulators, and RNA processors. Of these, CXXC5 through its CXXC domain interacted with EMD, MAZ, and MeCP2. Furthermore, an interplay between CXXC5 and MeCP2 was critical for a subset of CXXC5 target gene expressions. It appears that CXXC5 may act as a nucleation factor in modulating gene expressions. Providing a prelude for CXXC5 actions, our results could also contribute to a better understanding of CXXC5-mediated cellular processes in physiology and pathophysiology.Gamze AyazGizem TuranÇağla Ece OlgunGizem KarsBurcu KarakayaKerim YavuzÖykü Deniz DemiralayTolga CanMesut MuyanPelin YaşarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gamze Ayaz Gizem Turan Çağla Ece Olgun Gizem Kars Burcu Karakaya Kerim Yavuz Öykü Deniz Demiralay Tolga Can Mesut Muyan Pelin Yaşar A prelude to the proximity interaction mapping of CXXC5
description	Abstract CXXC5 is a member of the zinc-finger CXXC family proteins that interact with unmodified CpG dinucleotides through a conserved ZF-CXXC domain. CXXC5 is involved in the modulation of gene expressions that lead to alterations in diverse cellular events. However, the underlying mechanism of CXXC5-modulated gene expressions remains unclear. Proteins perform their functions in a network of proteins whose identities and amounts change spatiotemporally in response to various stimuli in a lineage-specific manner. Since CXXC5 lacks an intrinsic transcription regulatory function or enzymatic activity but is a DNA binder, CXXC5 by interacting with proteins could act as a scaffold to establish a chromatin state restrictive or permissive for transcription. To initially address this, we utilized the proximity-dependent biotinylation approach. Proximity interaction partners of CXXC5 include DNA and chromatin modifiers, transcription factors/co-regulators, and RNA processors. Of these, CXXC5 through its CXXC domain interacted with EMD, MAZ, and MeCP2. Furthermore, an interplay between CXXC5 and MeCP2 was critical for a subset of CXXC5 target gene expressions. It appears that CXXC5 may act as a nucleation factor in modulating gene expressions. Providing a prelude for CXXC5 actions, our results could also contribute to a better understanding of CXXC5-mediated cellular processes in physiology and pathophysiology.
format	article
author	Gamze Ayaz Gizem Turan Çağla Ece Olgun Gizem Kars Burcu Karakaya Kerim Yavuz Öykü Deniz Demiralay Tolga Can Mesut Muyan Pelin Yaşar
author_facet	Gamze Ayaz Gizem Turan Çağla Ece Olgun Gizem Kars Burcu Karakaya Kerim Yavuz Öykü Deniz Demiralay Tolga Can Mesut Muyan Pelin Yaşar
author_sort	Gamze Ayaz
title	A prelude to the proximity interaction mapping of CXXC5
title_short	A prelude to the proximity interaction mapping of CXXC5
title_full	A prelude to the proximity interaction mapping of CXXC5
title_fullStr	A prelude to the proximity interaction mapping of CXXC5
title_full_unstemmed	A prelude to the proximity interaction mapping of CXXC5
title_sort	prelude to the proximity interaction mapping of cxxc5
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/75e2878109d14e88b1641504d87a2085
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A prelude to the proximity interaction mapping of CXXC5

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