Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation

Summary: The importance of the G-protein βγ subunits in the regulation of cargo transport from the trans-Golgi network (TGN) to the plasma membrane (PM) is well accepted; however, the molecular mechanism underlying the G-protein activation at the TGN remains unclear. We show here that sphingosine 1-...

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Autores principales:	Taro Okada, Susumu Nishida, Lifang Zhang, Nesma Nabil Ibrahim Mohamed, Tianyou Wang, Takeshi Ijuin, Taketoshi Kajimoto, Shun-Ichi Nakamura
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell biology Organizational aspects of cell biology Functional aspects of cell biology Science Q
Acceso en línea:	https://doaj.org/article/75eaec608d4b498a8de513a8224e8905
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spelling	oai:doaj.org-article:75eaec608d4b498a8de513a8224e89052021-11-20T05:10:23ZConstitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation2589-004210.1016/j.isci.2021.103351https://doaj.org/article/75eaec608d4b498a8de513a8224e89052021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221013201https://doaj.org/toc/2589-0042Summary: The importance of the G-protein βγ subunits in the regulation of cargo transport from the trans-Golgi network (TGN) to the plasma membrane (PM) is well accepted; however, the molecular mechanism underlying the G-protein activation at the TGN remains unclear. We show here that sphingosine 1-phosphate (S1P) receptors at the PM were trafficked to the TGN in response to a surface transport cargo, temperature-sensitive vesicular stomatitis virus glycoprotein tagged with green fluorescent protein accumulation in the Golgi. The receptor internalization occurred in an S1P-independent manner but required phosphorylation by G-protein receptor kinase 2 and β-arrestin association before internalization. Continuously activated S1P receptors in a manner dependent on S1P at the TGN kept transmitting G-protein signals including the βγ subunits supply necessary for transport carrier formation at the TGN destined for the PM.Taro OkadaSusumu NishidaLifang ZhangNesma Nabil Ibrahim MohamedTianyou WangTakeshi IjuinTaketoshi KajimotoShun-Ichi NakamuraElsevierarticleCell biologyOrganizational aspects of cell biologyFunctional aspects of cell biologyScienceQENiScience, Vol 24, Iss 11, Pp 103351- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell biology Organizational aspects of cell biology Functional aspects of cell biology Science Q
spellingShingle	Cell biology Organizational aspects of cell biology Functional aspects of cell biology Science Q Taro Okada Susumu Nishida Lifang Zhang Nesma Nabil Ibrahim Mohamed Tianyou Wang Takeshi Ijuin Taketoshi Kajimoto Shun-Ichi Nakamura Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation
description	Summary: The importance of the G-protein βγ subunits in the regulation of cargo transport from the trans-Golgi network (TGN) to the plasma membrane (PM) is well accepted; however, the molecular mechanism underlying the G-protein activation at the TGN remains unclear. We show here that sphingosine 1-phosphate (S1P) receptors at the PM were trafficked to the TGN in response to a surface transport cargo, temperature-sensitive vesicular stomatitis virus glycoprotein tagged with green fluorescent protein accumulation in the Golgi. The receptor internalization occurred in an S1P-independent manner but required phosphorylation by G-protein receptor kinase 2 and β-arrestin association before internalization. Continuously activated S1P receptors in a manner dependent on S1P at the TGN kept transmitting G-protein signals including the βγ subunits supply necessary for transport carrier formation at the TGN destined for the PM.
format	article
author	Taro Okada Susumu Nishida Lifang Zhang Nesma Nabil Ibrahim Mohamed Tianyou Wang Takeshi Ijuin Taketoshi Kajimoto Shun-Ichi Nakamura
author_facet	Taro Okada Susumu Nishida Lifang Zhang Nesma Nabil Ibrahim Mohamed Tianyou Wang Takeshi Ijuin Taketoshi Kajimoto Shun-Ichi Nakamura
author_sort	Taro Okada
title	Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation
title_short	Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation
title_full	Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation
title_fullStr	Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation
title_full_unstemmed	Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation
title_sort	constitutive activation of s1p receptors at the trans-golgi network is required for surface transport carrier formation
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/75eaec608d4b498a8de513a8224e8905
work_keys_str_mv	AT tarookada constitutiveactivationofs1preceptorsatthetransgolginetworkisrequiredforsurfacetransportcarrierformation AT susumunishida constitutiveactivationofs1preceptorsatthetransgolginetworkisrequiredforsurfacetransportcarrierformation AT lifangzhang constitutiveactivationofs1preceptorsatthetransgolginetworkisrequiredforsurfacetransportcarrierformation AT nesmanabilibrahimmohamed constitutiveactivationofs1preceptorsatthetransgolginetworkisrequiredforsurfacetransportcarrierformation AT tianyouwang constitutiveactivationofs1preceptorsatthetransgolginetworkisrequiredforsurfacetransportcarrierformation AT takeshiijuin constitutiveactivationofs1preceptorsatthetransgolginetworkisrequiredforsurfacetransportcarrierformation AT taketoshikajimoto constitutiveactivationofs1preceptorsatthetransgolginetworkisrequiredforsurfacetransportcarrierformation AT shunichinakamura constitutiveactivationofs1preceptorsatthetransgolginetworkisrequiredforsurfacetransportcarrierformation
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Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation

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