Exosomal Long Non-Coding RNA Expression from Serum of Patients with Acute Minor Stroke

Xiaonan Xu, 1,* Chengle Zhuang, 2,* Liming Chen 1 1Department of Neurology, Shenzhen University General Hospital, Shenzhen 518000, Guangdong Province, People’s Republic of China; 2Department of Urology, Peking University Shenzhen Hospital, Shenzhen 518000, Guangdong Province, People&...

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Autores principales:	Xu X, Zhuang C, Chen L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	exosomes stroke lncrnas biomarker Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/75f5f3756f7a40ec999c37ff70236125
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spelling	oai:doaj.org-article:75f5f3756f7a40ec999c37ff702361252021-12-02T06:38:13ZExosomal Long Non-Coding RNA Expression from Serum of Patients with Acute Minor Stroke1178-2021https://doaj.org/article/75f5f3756f7a40ec999c37ff702361252020-01-01T00:00:00Zhttps://www.dovepress.com/exosomal-long-non-coding-rna-expression-from-serum-of-patients-with-ac-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Xiaonan Xu, 1,* Chengle Zhuang, 2,* Liming Chen 1 1Department of Neurology, Shenzhen University General Hospital, Shenzhen 518000, Guangdong Province, People’s Republic of China; 2Department of Urology, Peking University Shenzhen Hospital, Shenzhen 518000, Guangdong Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Liming Chen Email limingchen_2000@sina.comBackground: Acute minor stroke (AMS) is one kind of hypoxic ischemic necrosis with no more than 4 National Institutes of Health Stroke Scale (NIHSS) score. However, the early diagnosis of AMS is tough for lack of effective molecular markers. Recently, many long non-coding RNAs (lncRNAs) associated with AMS have been gradually revealed. Here, we aim to find the potential biomarkers of lncRNAs in exosomes isolated from blood serum of patients with AMS for early detection.Methods: RNA-seq technique, KEGG pathway analysis and GO enrichment analysis were used in this study. Besides, reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) was used to validate expression levels of four of eleven differentially expressed lncRNAs (lnc-CRKL-2, lnc-NTRK3-4, RPS6KA2-AS1 and lnc-CALM1-7) involved in the neurotrophin signaling pathway.Results: The expression levels of lnc-CRKL-2 (mean value 48, standard deviation 4.583, P = 0.003) and lnc-NTRK3-4 (mean value 32.3, standard deviation 2.08, P = 0.001) were increased significantly in AMS patients, while the expression levels of RPS6KA2-AS1 (mean value − 118.7, standard deviation 7.09, P = 0.001) and lnc-CALM1-7 (mean value − 148.7, standard deviation 6.10, P = 0.001) were decreased dramatically.Conclusion: In conclusion, these four new revealed lncRNAs may be used as novel joint biomarkers for the early detection of AMS.Keywords: exosomes, stroke, LncRNAs, biomarker  Xu XZhuang CChen LDove Medical PressarticleexosomesstrokelncrnasbiomarkerNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 16, Pp 153-160 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	exosomes stroke lncrnas biomarker Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	exosomes stroke lncrnas biomarker Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Xu X Zhuang C Chen L Exosomal Long Non-Coding RNA Expression from Serum of Patients with Acute Minor Stroke
description	Xiaonan Xu, 1,* Chengle Zhuang, 2,* Liming Chen 1 1Department of Neurology, Shenzhen University General Hospital, Shenzhen 518000, Guangdong Province, People’s Republic of China; 2Department of Urology, Peking University Shenzhen Hospital, Shenzhen 518000, Guangdong Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Liming Chen Email limingchen_2000@sina.comBackground: Acute minor stroke (AMS) is one kind of hypoxic ischemic necrosis with no more than 4 National Institutes of Health Stroke Scale (NIHSS) score. However, the early diagnosis of AMS is tough for lack of effective molecular markers. Recently, many long non-coding RNAs (lncRNAs) associated with AMS have been gradually revealed. Here, we aim to find the potential biomarkers of lncRNAs in exosomes isolated from blood serum of patients with AMS for early detection.Methods: RNA-seq technique, KEGG pathway analysis and GO enrichment analysis were used in this study. Besides, reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) was used to validate expression levels of four of eleven differentially expressed lncRNAs (lnc-CRKL-2, lnc-NTRK3-4, RPS6KA2-AS1 and lnc-CALM1-7) involved in the neurotrophin signaling pathway.Results: The expression levels of lnc-CRKL-2 (mean value 48, standard deviation 4.583, P = 0.003) and lnc-NTRK3-4 (mean value 32.3, standard deviation 2.08, P = 0.001) were increased significantly in AMS patients, while the expression levels of RPS6KA2-AS1 (mean value − 118.7, standard deviation 7.09, P = 0.001) and lnc-CALM1-7 (mean value − 148.7, standard deviation 6.10, P = 0.001) were decreased dramatically.Conclusion: In conclusion, these four new revealed lncRNAs may be used as novel joint biomarkers for the early detection of AMS.Keywords: exosomes, stroke, LncRNAs, biomarker
format	article
author	Xu X Zhuang C Chen L
author_facet	Xu X Zhuang C Chen L
author_sort	Xu X
title	Exosomal Long Non-Coding RNA Expression from Serum of Patients with Acute Minor Stroke
title_short	Exosomal Long Non-Coding RNA Expression from Serum of Patients with Acute Minor Stroke
title_full	Exosomal Long Non-Coding RNA Expression from Serum of Patients with Acute Minor Stroke
title_fullStr	Exosomal Long Non-Coding RNA Expression from Serum of Patients with Acute Minor Stroke
title_full_unstemmed	Exosomal Long Non-Coding RNA Expression from Serum of Patients with Acute Minor Stroke
title_sort	exosomal long non-coding rna expression from serum of patients with acute minor stroke
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/75f5f3756f7a40ec999c37ff70236125
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Exosomal Long Non-Coding RNA Expression from Serum of Patients with Acute Minor Stroke

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