Cyclomodulins and Hemolysis in <i>E. coli</i> as Potential Low-Cost Non-Invasive Biomarkers for Colorectal Cancer Screening

The frequent occurrence of <i>E. coli</i> positive for cyclomodulins such as colibactin (CLB), the cytotoxic necrotizing factor (CNF), and the cytolethal distending factor (CDT) in colorectal cancer (CRC) patients published so far provides the opportunity to use them as CRC screening mar...

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Autores principales:	Kristýna Mezerová, Lubomír Starý, Pavel Zbořil, Ivo Klementa, Martin Stašek, Petr Špička, Pavel Skalický, Vladislav Raclavský
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	colorectal cancer screening genotoxin colibactin cytotoxic necrotizing factor Science Q
Acceso en línea:	https://doaj.org/article/75f7969888ce4dd487748e79e64df620
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spelling	oai:doaj.org-article:75f7969888ce4dd487748e79e64df6202021-11-25T18:10:50ZCyclomodulins and Hemolysis in <i>E. coli</i> as Potential Low-Cost Non-Invasive Biomarkers for Colorectal Cancer Screening10.3390/life111111652075-1729https://doaj.org/article/75f7969888ce4dd487748e79e64df6202021-10-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1165https://doaj.org/toc/2075-1729The frequent occurrence of <i>E. coli</i> positive for cyclomodulins such as colibactin (CLB), the cytotoxic necrotizing factor (CNF), and the cytolethal distending factor (CDT) in colorectal cancer (CRC) patients published so far provides the opportunity to use them as CRC screening markers. We examined the practicability and performance of a low-cost detection approach that relied on culture followed by simplified DNA extraction and PCR in <i>E. coli</i> isolates recovered from 130 CRC patients and 111 controls. Our results showed a statistically significant association between CRC and the presence of colibactin genes <i>clbB</i> and <i>clbN</i>, the <i>cnf</i> gene, and newly, the hemolytic phenotype of <i>E. coli</i> isolates. We also observed a significant increase in the mean number of morphologically distinct <i>E. coli</i> isolates per patient in the CRC cohort compared to controls, indicating that the cyclomodulin-producing <i>E. coli</i> strains may represent potentially preventable harmful newcomers in CRC patients. A colibactin gene assay showed the highest detection rate (45.4%), and males would benefit from the screening more than females. However, because of the high number of false positives, practical use of this marker must be explored. In our opinion, it may serve as an auxiliary marker to increase the specificity and/or sensitivity of the well-established fecal immunochemical test (FIT) in CRC screening.Kristýna MezerováLubomír StarýPavel ZbořilIvo KlementaMartin StašekPetr ŠpičkaPavel SkalickýVladislav RaclavskýMDPI AGarticlecolorectal cancerscreeninggenotoxincolibactincytotoxic necrotizing factorScienceQENLife, Vol 11, Iss 1165, p 1165 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	colorectal cancer screening genotoxin colibactin cytotoxic necrotizing factor Science Q
spellingShingle	colorectal cancer screening genotoxin colibactin cytotoxic necrotizing factor Science Q Kristýna Mezerová Lubomír Starý Pavel Zbořil Ivo Klementa Martin Stašek Petr Špička Pavel Skalický Vladislav Raclavský Cyclomodulins and Hemolysis in <i>E. coli</i> as Potential Low-Cost Non-Invasive Biomarkers for Colorectal Cancer Screening
description	The frequent occurrence of <i>E. coli</i> positive for cyclomodulins such as colibactin (CLB), the cytotoxic necrotizing factor (CNF), and the cytolethal distending factor (CDT) in colorectal cancer (CRC) patients published so far provides the opportunity to use them as CRC screening markers. We examined the practicability and performance of a low-cost detection approach that relied on culture followed by simplified DNA extraction and PCR in <i>E. coli</i> isolates recovered from 130 CRC patients and 111 controls. Our results showed a statistically significant association between CRC and the presence of colibactin genes <i>clbB</i> and <i>clbN</i>, the <i>cnf</i> gene, and newly, the hemolytic phenotype of <i>E. coli</i> isolates. We also observed a significant increase in the mean number of morphologically distinct <i>E. coli</i> isolates per patient in the CRC cohort compared to controls, indicating that the cyclomodulin-producing <i>E. coli</i> strains may represent potentially preventable harmful newcomers in CRC patients. A colibactin gene assay showed the highest detection rate (45.4%), and males would benefit from the screening more than females. However, because of the high number of false positives, practical use of this marker must be explored. In our opinion, it may serve as an auxiliary marker to increase the specificity and/or sensitivity of the well-established fecal immunochemical test (FIT) in CRC screening.
format	article
author	Kristýna Mezerová Lubomír Starý Pavel Zbořil Ivo Klementa Martin Stašek Petr Špička Pavel Skalický Vladislav Raclavský
author_facet	Kristýna Mezerová Lubomír Starý Pavel Zbořil Ivo Klementa Martin Stašek Petr Špička Pavel Skalický Vladislav Raclavský
author_sort	Kristýna Mezerová
title	Cyclomodulins and Hemolysis in <i>E. coli</i> as Potential Low-Cost Non-Invasive Biomarkers for Colorectal Cancer Screening
title_short	Cyclomodulins and Hemolysis in <i>E. coli</i> as Potential Low-Cost Non-Invasive Biomarkers for Colorectal Cancer Screening
title_full	Cyclomodulins and Hemolysis in <i>E. coli</i> as Potential Low-Cost Non-Invasive Biomarkers for Colorectal Cancer Screening
title_fullStr	Cyclomodulins and Hemolysis in <i>E. coli</i> as Potential Low-Cost Non-Invasive Biomarkers for Colorectal Cancer Screening
title_full_unstemmed	Cyclomodulins and Hemolysis in <i>E. coli</i> as Potential Low-Cost Non-Invasive Biomarkers for Colorectal Cancer Screening
title_sort	cyclomodulins and hemolysis in <i>e. coli</i> as potential low-cost non-invasive biomarkers for colorectal cancer screening
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/75f7969888ce4dd487748e79e64df620
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Cyclomodulins and Hemolysis in <i>E. coli</i> as Potential Low-Cost Non-Invasive Biomarkers for Colorectal Cancer Screening

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