Single molecule secondary structure determination of proteins through infrared absorption nanospectroscopy

While infrared nanospectroscopy methods based on thermomechanical detection (AFM-IR) enables the acquisition of absorption spectra at the nanoscale, single molecule detection has not been possible so far. Here, the authors present off-resonance, low power and short pulse infrared nanospectroscopy (O...

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Autores principales:	Francesco Simone Ruggeri, Benedetta Mannini, Roman Schmid, Michele Vendruscolo, Tuomas P. J. Knowles
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/75fd9014aacf4a8eacc2ca3e369c4d03
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spelling	oai:doaj.org-article:75fd9014aacf4a8eacc2ca3e369c4d032021-12-02T17:30:52ZSingle molecule secondary structure determination of proteins through infrared absorption nanospectroscopy10.1038/s41467-020-16728-12041-1723https://doaj.org/article/75fd9014aacf4a8eacc2ca3e369c4d032020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16728-1https://doaj.org/toc/2041-1723While infrared nanospectroscopy methods based on thermomechanical detection (AFM-IR) enables the acquisition of absorption spectra at the nanoscale, single molecule detection has not been possible so far. Here, the authors present off-resonance, low power and short pulse infrared nanospectroscopy (ORS-nanoIR), which allows measuring infrared absorption spectra at the single molecule level in a time scale of seconds with high throughput and demonstrate that the secondary structure of single protein molecules can be determined with this method.Francesco Simone RuggeriBenedetta ManniniRoman SchmidMichele VendruscoloTuomas P. J. KnowlesNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Francesco Simone Ruggeri Benedetta Mannini Roman Schmid Michele Vendruscolo Tuomas P. J. Knowles Single molecule secondary structure determination of proteins through infrared absorption nanospectroscopy
description	While infrared nanospectroscopy methods based on thermomechanical detection (AFM-IR) enables the acquisition of absorption spectra at the nanoscale, single molecule detection has not been possible so far. Here, the authors present off-resonance, low power and short pulse infrared nanospectroscopy (ORS-nanoIR), which allows measuring infrared absorption spectra at the single molecule level in a time scale of seconds with high throughput and demonstrate that the secondary structure of single protein molecules can be determined with this method.
format	article
author	Francesco Simone Ruggeri Benedetta Mannini Roman Schmid Michele Vendruscolo Tuomas P. J. Knowles
author_facet	Francesco Simone Ruggeri Benedetta Mannini Roman Schmid Michele Vendruscolo Tuomas P. J. Knowles
author_sort	Francesco Simone Ruggeri
title	Single molecule secondary structure determination of proteins through infrared absorption nanospectroscopy
title_short	Single molecule secondary structure determination of proteins through infrared absorption nanospectroscopy
title_full	Single molecule secondary structure determination of proteins through infrared absorption nanospectroscopy
title_fullStr	Single molecule secondary structure determination of proteins through infrared absorption nanospectroscopy
title_full_unstemmed	Single molecule secondary structure determination of proteins through infrared absorption nanospectroscopy
title_sort	single molecule secondary structure determination of proteins through infrared absorption nanospectroscopy
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/75fd9014aacf4a8eacc2ca3e369c4d03
work_keys_str_mv	AT francescosimoneruggeri singlemoleculesecondarystructuredeterminationofproteinsthroughinfraredabsorptionnanospectroscopy AT benedettamannini singlemoleculesecondarystructuredeterminationofproteinsthroughinfraredabsorptionnanospectroscopy AT romanschmid singlemoleculesecondarystructuredeterminationofproteinsthroughinfraredabsorptionnanospectroscopy AT michelevendruscolo singlemoleculesecondarystructuredeterminationofproteinsthroughinfraredabsorptionnanospectroscopy AT tuomaspjknowles singlemoleculesecondarystructuredeterminationofproteinsthroughinfraredabsorptionnanospectroscopy
_version_	1718380734677254144

Single molecule secondary structure determination of proteins through infrared absorption nanospectroscopy

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