Docking of LDCVs is modulated by lower intracellular [Ca2+] than priming.

Many regulatory steps precede final membrane fusion in neuroendocrine cells. Some parts of this preparatory cascade, including fusion and priming, are dependent on the intracellular Ca(2+) concentration ([Ca(2+)](i)). However, the functional implications of [Ca(2+)](i) in the regulation of docking r...

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Autores principales:	Mathias Pasche, Ulf Matti, Detlef Hof, Jens Rettig, Ute Becherer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/760802b86de0484a81d9c831a5ce02de
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spelling	oai:doaj.org-article:760802b86de0484a81d9c831a5ce02de2021-11-18T07:19:12ZDocking of LDCVs is modulated by lower intracellular [Ca2+] than priming.1932-620310.1371/journal.pone.0036416https://doaj.org/article/760802b86de0484a81d9c831a5ce02de2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22590540/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Many regulatory steps precede final membrane fusion in neuroendocrine cells. Some parts of this preparatory cascade, including fusion and priming, are dependent on the intracellular Ca(2+) concentration ([Ca(2+)](i)). However, the functional implications of [Ca(2+)](i) in the regulation of docking remain elusive and controversial due to an inability to determine the modulatory effect of [Ca(2+)](i). Using a combination of TIRF-microscopy and electrophysiology we followed the movement of large dense core vesicles (LDCVs) close to the plasma membrane, simultaneously measuring membrane capacitance and [Ca(2+)](i). We found that a free [Ca(2+)](i) of 700 nM maximized the immediately releasable pool and minimized the lateral mobility of vesicles, which is consistent with a maximal increase of the pool size of primed LDCVs. The parameters that reflect docking, i.e. axial mobility and the fraction of LDCVs residing at the plasma membrane for less than 5 seconds, were strongly decreased at a free [Ca(2+)](i) of 500 nM. These results provide the first evidence that docking and priming occur at different free intracellular Ca(2+) concentrations, with docking efficiency being the most robust at 500 nM.Mathias PascheUlf MattiDetlef HofJens RettigUte BechererPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36416 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mathias Pasche Ulf Matti Detlef Hof Jens Rettig Ute Becherer Docking of LDCVs is modulated by lower intracellular [Ca2+] than priming.
description	Many regulatory steps precede final membrane fusion in neuroendocrine cells. Some parts of this preparatory cascade, including fusion and priming, are dependent on the intracellular Ca(2+) concentration ([Ca(2+)](i)). However, the functional implications of [Ca(2+)](i) in the regulation of docking remain elusive and controversial due to an inability to determine the modulatory effect of [Ca(2+)](i). Using a combination of TIRF-microscopy and electrophysiology we followed the movement of large dense core vesicles (LDCVs) close to the plasma membrane, simultaneously measuring membrane capacitance and [Ca(2+)](i). We found that a free [Ca(2+)](i) of 700 nM maximized the immediately releasable pool and minimized the lateral mobility of vesicles, which is consistent with a maximal increase of the pool size of primed LDCVs. The parameters that reflect docking, i.e. axial mobility and the fraction of LDCVs residing at the plasma membrane for less than 5 seconds, were strongly decreased at a free [Ca(2+)](i) of 500 nM. These results provide the first evidence that docking and priming occur at different free intracellular Ca(2+) concentrations, with docking efficiency being the most robust at 500 nM.
format	article
author	Mathias Pasche Ulf Matti Detlef Hof Jens Rettig Ute Becherer
author_facet	Mathias Pasche Ulf Matti Detlef Hof Jens Rettig Ute Becherer
author_sort	Mathias Pasche
title	Docking of LDCVs is modulated by lower intracellular [Ca2+] than priming.
title_short	Docking of LDCVs is modulated by lower intracellular [Ca2+] than priming.
title_full	Docking of LDCVs is modulated by lower intracellular [Ca2+] than priming.
title_fullStr	Docking of LDCVs is modulated by lower intracellular [Ca2+] than priming.
title_full_unstemmed	Docking of LDCVs is modulated by lower intracellular [Ca2+] than priming.
title_sort	docking of ldcvs is modulated by lower intracellular [ca2+] than priming.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/760802b86de0484a81d9c831a5ce02de
work_keys_str_mv	AT mathiaspasche dockingofldcvsismodulatedbylowerintracellularca2thanpriming AT ulfmatti dockingofldcvsismodulatedbylowerintracellularca2thanpriming AT detlefhof dockingofldcvsismodulatedbylowerintracellularca2thanpriming AT jensrettig dockingofldcvsismodulatedbylowerintracellularca2thanpriming AT utebecherer dockingofldcvsismodulatedbylowerintracellularca2thanpriming
_version_	1718423605892612096

Docking of LDCVs is modulated by lower intracellular [Ca2+] than priming.

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