Camera-based localization microscopy optimized with calibrated structured illumination
The structure and processes of life’s molecules at the nanoscale are probed with optical super resolution techniques. The authors present a method that combines conventional localization information and information from structured illumination, which outperforms other methods by localizing single mo...
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Nature Portfolio
2021
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oai:doaj.org-article:76106148508b480582066e892b5fc54c2021-12-02T15:52:57ZCamera-based localization microscopy optimized with calibrated structured illumination10.1038/s42005-021-00546-y2399-3650https://doaj.org/article/76106148508b480582066e892b5fc54c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s42005-021-00546-yhttps://doaj.org/toc/2399-3650The structure and processes of life’s molecules at the nanoscale are probed with optical super resolution techniques. The authors present a method that combines conventional localization information and information from structured illumination, which outperforms other methods by localizing single molecules with a theoretically optimal doubling of precision.Martin SchmidtAdam C. HundahlHenrik FlyvbjergRodolphe MarieKim I. MortensenNature PortfolioarticleAstrophysicsQB460-466PhysicsQC1-999ENCommunications Physics, Vol 4, Iss 1, Pp 1-9 (2021) |
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Astrophysics QB460-466 Physics QC1-999 Martin Schmidt Adam C. Hundahl Henrik Flyvbjerg Rodolphe Marie Kim I. Mortensen Camera-based localization microscopy optimized with calibrated structured illumination |
description |
The structure and processes of life’s molecules at the nanoscale are probed with optical super resolution techniques. The authors present a method that combines conventional localization information and information from structured illumination, which outperforms other methods by localizing single molecules with a theoretically optimal doubling of precision. |
format |
article |
author |
Martin Schmidt Adam C. Hundahl Henrik Flyvbjerg Rodolphe Marie Kim I. Mortensen |
author_facet |
Martin Schmidt Adam C. Hundahl Henrik Flyvbjerg Rodolphe Marie Kim I. Mortensen |
author_sort |
Martin Schmidt |
title |
Camera-based localization microscopy optimized with calibrated structured illumination |
title_short |
Camera-based localization microscopy optimized with calibrated structured illumination |
title_full |
Camera-based localization microscopy optimized with calibrated structured illumination |
title_fullStr |
Camera-based localization microscopy optimized with calibrated structured illumination |
title_full_unstemmed |
Camera-based localization microscopy optimized with calibrated structured illumination |
title_sort |
camera-based localization microscopy optimized with calibrated structured illumination |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/76106148508b480582066e892b5fc54c |
work_keys_str_mv |
AT martinschmidt camerabasedlocalizationmicroscopyoptimizedwithcalibratedstructuredillumination AT adamchundahl camerabasedlocalizationmicroscopyoptimizedwithcalibratedstructuredillumination AT henrikflyvbjerg camerabasedlocalizationmicroscopyoptimizedwithcalibratedstructuredillumination AT rodolphemarie camerabasedlocalizationmicroscopyoptimizedwithcalibratedstructuredillumination AT kimimortensen camerabasedlocalizationmicroscopyoptimizedwithcalibratedstructuredillumination |
_version_ |
1718385578431479808 |