Camera-based localization microscopy optimized with calibrated structured illumination

The structure and processes of life’s molecules at the nanoscale are probed with optical super resolution techniques. The authors present a method that combines conventional localization information and information from structured illumination, which outperforms other methods by localizing single mo...

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Autores principales: Martin Schmidt, Adam C. Hundahl, Henrik Flyvbjerg, Rodolphe Marie, Kim I. Mortensen
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/76106148508b480582066e892b5fc54c
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spelling oai:doaj.org-article:76106148508b480582066e892b5fc54c2021-12-02T15:52:57ZCamera-based localization microscopy optimized with calibrated structured illumination10.1038/s42005-021-00546-y2399-3650https://doaj.org/article/76106148508b480582066e892b5fc54c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s42005-021-00546-yhttps://doaj.org/toc/2399-3650The structure and processes of life’s molecules at the nanoscale are probed with optical super resolution techniques. The authors present a method that combines conventional localization information and information from structured illumination, which outperforms other methods by localizing single molecules with a theoretically optimal doubling of precision.Martin SchmidtAdam C. HundahlHenrik FlyvbjergRodolphe MarieKim I. MortensenNature PortfolioarticleAstrophysicsQB460-466PhysicsQC1-999ENCommunications Physics, Vol 4, Iss 1, Pp 1-9 (2021)
institution DOAJ
collection DOAJ
language EN
topic Astrophysics
QB460-466
Physics
QC1-999
spellingShingle Astrophysics
QB460-466
Physics
QC1-999
Martin Schmidt
Adam C. Hundahl
Henrik Flyvbjerg
Rodolphe Marie
Kim I. Mortensen
Camera-based localization microscopy optimized with calibrated structured illumination
description The structure and processes of life’s molecules at the nanoscale are probed with optical super resolution techniques. The authors present a method that combines conventional localization information and information from structured illumination, which outperforms other methods by localizing single molecules with a theoretically optimal doubling of precision.
format article
author Martin Schmidt
Adam C. Hundahl
Henrik Flyvbjerg
Rodolphe Marie
Kim I. Mortensen
author_facet Martin Schmidt
Adam C. Hundahl
Henrik Flyvbjerg
Rodolphe Marie
Kim I. Mortensen
author_sort Martin Schmidt
title Camera-based localization microscopy optimized with calibrated structured illumination
title_short Camera-based localization microscopy optimized with calibrated structured illumination
title_full Camera-based localization microscopy optimized with calibrated structured illumination
title_fullStr Camera-based localization microscopy optimized with calibrated structured illumination
title_full_unstemmed Camera-based localization microscopy optimized with calibrated structured illumination
title_sort camera-based localization microscopy optimized with calibrated structured illumination
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/76106148508b480582066e892b5fc54c
work_keys_str_mv AT martinschmidt camerabasedlocalizationmicroscopyoptimizedwithcalibratedstructuredillumination
AT adamchundahl camerabasedlocalizationmicroscopyoptimizedwithcalibratedstructuredillumination
AT henrikflyvbjerg camerabasedlocalizationmicroscopyoptimizedwithcalibratedstructuredillumination
AT rodolphemarie camerabasedlocalizationmicroscopyoptimizedwithcalibratedstructuredillumination
AT kimimortensen camerabasedlocalizationmicroscopyoptimizedwithcalibratedstructuredillumination
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