Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris

In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm Sy...

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Autores principales:	Marjanović S. Đorđe, Bogunović Danica, Milovanović Mirjana, Marinković Darko, Zdravković Nemanja, Magaš Vladimir, Trailović M. Saša
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	c. elegans s. muris carvacrol thymol piperazine Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/7614394eafab4959a54811e8d027c277
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spelling	oai:doaj.org-article:7614394eafab4959a54811e8d027c2772021-11-17T21:27:53ZAntihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris1820-744810.2478/acve-2018-0036https://doaj.org/article/7614394eafab4959a54811e8d027c2772018-12-01T00:00:00Zhttps://doi.org/10.2478/acve-2018-0036https://doaj.org/toc/1820-7448In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm Syphacia muris. The control treatment of the infected rats was carried out with piperazine. Thymol caused a dose and time-dependent mortality in adult C. elegans. The value of the Median Lethal Concentration (LC50) of thymol was 117.9nM after 24h and 62.89 nM after 48h of exposure. Carvacrol exhibited a higher antinematodal efficiency than thymol. The LC50 of carvacrol, after 24 hours of exposure, was 53.03 nM, while after 48 hours it was 33.83 nM. On the other hand, piperazine showed an extremely high efficacy against S. muris infection in rats. Piperazine, at a dose of 625 mg/kg bw, administered in drinking water continuously for 10 days, eliminates the infection completely. However, none of the investigated active ingredients of essential oils were effective against S. muris. The reason for the lack of efficiency may be due to their pharmacokinetic properties. A relatively low amount of, orally administered, active ingredients of essential oils reaches the distal segments of the gastrointestinal tract, where S. muris inhabits the gut (colon and cecum). The obtained results, on C. elegans, indicate a clear dose and time-dependent antinematodal effect of thymol and carvacrol. However, for clinical application, it is necessary to examine the efficacy of microencapsulated formulations with a controlled release of active ingredients of essential oils in certain parts of the gastrointestinal tract.Marjanović S. ĐorđeBogunović DanicaMilovanović MirjanaMarinković DarkoZdravković NemanjaMagaš VladimirTrailović M. SašaSciendoarticlec. eleganss. muriscarvacrolthymolpiperazineVeterinary medicineSF600-1100ENActa Veterinaria, Vol 68, Iss 4, Pp 445-456 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	c. elegans s. muris carvacrol thymol piperazine Veterinary medicine SF600-1100
spellingShingle	c. elegans s. muris carvacrol thymol piperazine Veterinary medicine SF600-1100 Marjanović S. Đorđe Bogunović Danica Milovanović Mirjana Marinković Darko Zdravković Nemanja Magaš Vladimir Trailović M. Saša Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris
description	In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm Syphacia muris. The control treatment of the infected rats was carried out with piperazine. Thymol caused a dose and time-dependent mortality in adult C. elegans. The value of the Median Lethal Concentration (LC50) of thymol was 117.9nM after 24h and 62.89 nM after 48h of exposure. Carvacrol exhibited a higher antinematodal efficiency than thymol. The LC50 of carvacrol, after 24 hours of exposure, was 53.03 nM, while after 48 hours it was 33.83 nM. On the other hand, piperazine showed an extremely high efficacy against S. muris infection in rats. Piperazine, at a dose of 625 mg/kg bw, administered in drinking water continuously for 10 days, eliminates the infection completely. However, none of the investigated active ingredients of essential oils were effective against S. muris. The reason for the lack of efficiency may be due to their pharmacokinetic properties. A relatively low amount of, orally administered, active ingredients of essential oils reaches the distal segments of the gastrointestinal tract, where S. muris inhabits the gut (colon and cecum). The obtained results, on C. elegans, indicate a clear dose and time-dependent antinematodal effect of thymol and carvacrol. However, for clinical application, it is necessary to examine the efficacy of microencapsulated formulations with a controlled release of active ingredients of essential oils in certain parts of the gastrointestinal tract.
format	article
author	Marjanović S. Đorđe Bogunović Danica Milovanović Mirjana Marinković Darko Zdravković Nemanja Magaš Vladimir Trailović M. Saša
author_facet	Marjanović S. Đorđe Bogunović Danica Milovanović Mirjana Marinković Darko Zdravković Nemanja Magaš Vladimir Trailović M. Saša
author_sort	Marjanović S. Đorđe
title	Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris
title_short	Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris
title_full	Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris
title_fullStr	Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris
title_full_unstemmed	Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris
title_sort	antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/7614394eafab4959a54811e8d027c277
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Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris

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