Discovery and validation of new potential biomarkers for early detection of colon cancer.

<h4>Background</h4>Accurate detection of characteristic proteins secreted by colon cancer tumor cells in biological fluids could serve as a biomarker for the disease. The aim of the present study was to identify and validate new serum biomarkers and demonstrate their potential usefulness...

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Autores principales:	Xavier Solé, Marta Crous-Bou, David Cordero, David Olivares, Elisabet Guinó, Rebeca Sanz-Pamplona, Francisco Rodriguez-Moranta, Xavier Sanjuan, Javier de Oca, Ramon Salazar, Victor Moreno
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/761aa31772e74ebbaf0fdc36a6585c81
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spelling	oai:doaj.org-article:761aa31772e74ebbaf0fdc36a6585c812021-11-25T06:00:46ZDiscovery and validation of new potential biomarkers for early detection of colon cancer.1932-620310.1371/journal.pone.0106748https://doaj.org/article/761aa31772e74ebbaf0fdc36a6585c812014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0106748https://doaj.org/toc/1932-6203<h4>Background</h4>Accurate detection of characteristic proteins secreted by colon cancer tumor cells in biological fluids could serve as a biomarker for the disease. The aim of the present study was to identify and validate new serum biomarkers and demonstrate their potential usefulness for early diagnosis of colon cancer.<h4>Methods</h4>The study was organized in three sequential phases: 1) biomarker discovery, 2) technical and biological validation, and 3) proof of concept to test the potential clinical use of selected biomarkers. A prioritized subset of the differentially-expressed genes between tissue types (50 colon mucosa from cancer-free individuals and 100 normal-tumor pairs from colon cancer patients) was validated and further tested in a series of serum samples from 80 colon cancer cases, 23 patients with adenoma and 77 cancer-free controls.<h4>Results</h4>In the discovery phase, 505 unique candidate biomarkers were identified, with highly significant results and high capacity to discriminate between the different tissue types. After a subsequent prioritization, all tested genes (N = 23) were successfully validated in tissue, and one of them, COL10A1, showed relevant differences in serum protein levels between controls, patients with adenoma (p = 0.0083) and colon cancer cases (p = 3.2e-6).<h4>Conclusion</h4>We present a sequential process for the identification and further validation of biomarkers for early detection of colon cancer that identifies COL10A1 protein levels in serum as a potential diagnostic candidate to detect both adenoma lesions and tumor.<h4>Impact</h4>The use of a cheap serum test for colon cancer screening should improve its participation rates and contribute to decrease the burden of this disease.Xavier SoléMarta Crous-BouDavid CorderoDavid OlivaresElisabet GuinóRebeca Sanz-PamplonaFrancisco Rodriguez-MorantaXavier SanjuanJavier de OcaRamon SalazarVictor MorenoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106748 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xavier Solé Marta Crous-Bou David Cordero David Olivares Elisabet Guinó Rebeca Sanz-Pamplona Francisco Rodriguez-Moranta Xavier Sanjuan Javier de Oca Ramon Salazar Victor Moreno Discovery and validation of new potential biomarkers for early detection of colon cancer.
description	<h4>Background</h4>Accurate detection of characteristic proteins secreted by colon cancer tumor cells in biological fluids could serve as a biomarker for the disease. The aim of the present study was to identify and validate new serum biomarkers and demonstrate their potential usefulness for early diagnosis of colon cancer.<h4>Methods</h4>The study was organized in three sequential phases: 1) biomarker discovery, 2) technical and biological validation, and 3) proof of concept to test the potential clinical use of selected biomarkers. A prioritized subset of the differentially-expressed genes between tissue types (50 colon mucosa from cancer-free individuals and 100 normal-tumor pairs from colon cancer patients) was validated and further tested in a series of serum samples from 80 colon cancer cases, 23 patients with adenoma and 77 cancer-free controls.<h4>Results</h4>In the discovery phase, 505 unique candidate biomarkers were identified, with highly significant results and high capacity to discriminate between the different tissue types. After a subsequent prioritization, all tested genes (N = 23) were successfully validated in tissue, and one of them, COL10A1, showed relevant differences in serum protein levels between controls, patients with adenoma (p = 0.0083) and colon cancer cases (p = 3.2e-6).<h4>Conclusion</h4>We present a sequential process for the identification and further validation of biomarkers for early detection of colon cancer that identifies COL10A1 protein levels in serum as a potential diagnostic candidate to detect both adenoma lesions and tumor.<h4>Impact</h4>The use of a cheap serum test for colon cancer screening should improve its participation rates and contribute to decrease the burden of this disease.
format	article
author	Xavier Solé Marta Crous-Bou David Cordero David Olivares Elisabet Guinó Rebeca Sanz-Pamplona Francisco Rodriguez-Moranta Xavier Sanjuan Javier de Oca Ramon Salazar Victor Moreno
author_facet	Xavier Solé Marta Crous-Bou David Cordero David Olivares Elisabet Guinó Rebeca Sanz-Pamplona Francisco Rodriguez-Moranta Xavier Sanjuan Javier de Oca Ramon Salazar Victor Moreno
author_sort	Xavier Solé
title	Discovery and validation of new potential biomarkers for early detection of colon cancer.
title_short	Discovery and validation of new potential biomarkers for early detection of colon cancer.
title_full	Discovery and validation of new potential biomarkers for early detection of colon cancer.
title_fullStr	Discovery and validation of new potential biomarkers for early detection of colon cancer.
title_full_unstemmed	Discovery and validation of new potential biomarkers for early detection of colon cancer.
title_sort	discovery and validation of new potential biomarkers for early detection of colon cancer.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/761aa31772e74ebbaf0fdc36a6585c81
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Discovery and validation of new potential biomarkers for early detection of colon cancer.

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