Genomic markers on synthetic genomes

Abstract Genome synthesis endows scientists the ability of de novo creating genomes absent in nature, by thorough redesigning DNA sequences and introducing numerous custom features. However, the genome synthesis is a labor‐ and time‐consuming work, and thus it is a challenge to verify and quantify t...

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Autores principales:	Hao‐Qian Zhao, Wen‐Qing Wei, Chao Zhao, Ze‐Xiong Xie
Formato:	article
Lenguaje:	EN
Publicado:	Wiley-VCH 2021
Materias:	genomic marker PCRTag recoding synthetic genome watermark Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/761c931bce394f1792648f26ce8a00f8
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id	oai:doaj.org-article:761c931bce394f1792648f26ce8a00f8
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spelling	oai:doaj.org-article:761c931bce394f1792648f26ce8a00f82021-12-02T13:20:01ZGenomic markers on synthetic genomes1618-28631618-024010.1002/elsc.202100030https://doaj.org/article/761c931bce394f1792648f26ce8a00f82021-12-01T00:00:00Zhttps://doi.org/10.1002/elsc.202100030https://doaj.org/toc/1618-0240https://doaj.org/toc/1618-2863Abstract Genome synthesis endows scientists the ability of de novo creating genomes absent in nature, by thorough redesigning DNA sequences and introducing numerous custom features. However, the genome synthesis is a labor‐ and time‐consuming work, and thus it is a challenge to verify and quantify the synthetic genome rapidly and precisely. Thus, specific DNA sequences different from native genomic sequences are designed into synthetic genomes during synthesis, namely genomic markers. Genomic markers can be easily detected by PCR reaction, whole‐genome sequencing (WGS) and a variety of methods to identify the synthetic genome from native one. Here, we review types and applications of genomic markers utilized in synthetic genomes, with the hope of providing a guidance for future works.Hao‐Qian ZhaoWen‐Qing WeiChao ZhaoZe‐Xiong XieWiley-VCHarticlegenomic markerPCRTagrecodingsynthetic genomewatermarkBiotechnologyTP248.13-248.65ENEngineering in Life Sciences, Vol 21, Iss 12, Pp 825-831 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	genomic marker PCRTag recoding synthetic genome watermark Biotechnology TP248.13-248.65
spellingShingle	genomic marker PCRTag recoding synthetic genome watermark Biotechnology TP248.13-248.65 Hao‐Qian Zhao Wen‐Qing Wei Chao Zhao Ze‐Xiong Xie Genomic markers on synthetic genomes
description	Abstract Genome synthesis endows scientists the ability of de novo creating genomes absent in nature, by thorough redesigning DNA sequences and introducing numerous custom features. However, the genome synthesis is a labor‐ and time‐consuming work, and thus it is a challenge to verify and quantify the synthetic genome rapidly and precisely. Thus, specific DNA sequences different from native genomic sequences are designed into synthetic genomes during synthesis, namely genomic markers. Genomic markers can be easily detected by PCR reaction, whole‐genome sequencing (WGS) and a variety of methods to identify the synthetic genome from native one. Here, we review types and applications of genomic markers utilized in synthetic genomes, with the hope of providing a guidance for future works.
format	article
author	Hao‐Qian Zhao Wen‐Qing Wei Chao Zhao Ze‐Xiong Xie
author_facet	Hao‐Qian Zhao Wen‐Qing Wei Chao Zhao Ze‐Xiong Xie
author_sort	Hao‐Qian Zhao
title	Genomic markers on synthetic genomes
title_short	Genomic markers on synthetic genomes
title_full	Genomic markers on synthetic genomes
title_fullStr	Genomic markers on synthetic genomes
title_full_unstemmed	Genomic markers on synthetic genomes
title_sort	genomic markers on synthetic genomes
publisher	Wiley-VCH
publishDate	2021
url	https://doaj.org/article/761c931bce394f1792648f26ce8a00f8
work_keys_str_mv	AT haoqianzhao genomicmarkersonsyntheticgenomes AT wenqingwei genomicmarkersonsyntheticgenomes AT chaozhao genomicmarkersonsyntheticgenomes AT zexiongxie genomicmarkersonsyntheticgenomes
_version_	1718393281150189568

Genomic markers on synthetic genomes

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