Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines
Background One of the most frequently deleted genes in cancer is CDKN2A encoding p16. This protein is often overexpressed in senescent cells, while its suppression can bypass the oncogene-induced senescence to enable transformation and tumorigenesis. The roles of the protein p16 are recently being e...
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PeerJ Inc.
2021
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oai:doaj.org-article:761dfcb95a51440793c3a9df063a1ddb2021-11-04T15:05:18ZDifferential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines10.7717/peerj.123582167-8359https://doaj.org/article/761dfcb95a51440793c3a9df063a1ddb2021-11-01T00:00:00Zhttps://peerj.com/articles/12358.pdfhttps://peerj.com/articles/12358/https://doaj.org/toc/2167-8359Background One of the most frequently deleted genes in cancer is CDKN2A encoding p16. This protein is often overexpressed in senescent cells, while its suppression can bypass the oncogene-induced senescence to enable transformation and tumorigenesis. The roles of the protein p16 are recently being expanded from the cell cycle progression regulator to the cellular regulator interacting in several different pathways. Yet data on its liver and liver cells’ expression are inconclusive. Methods The expression of the p16 gene in liver and liver cells was determined by RT-qPCR and compared to its protein amounts by western blotting. Results p16 is expressed at low levels in the liver and rat hepatocytes. Its expression varies from none to the considerable levels in the examined hepatocellular carcinoma cell lines (FaO and HepG2) and in immortalized mouse hepatocytes. Such significant expression differences of an important cellular regulator warrant the need to closely examine the differences in biochemical pathways correlated with the p16 expression when using hepatocytes and hepatoma liver models.Barbara KramarDušan ŠuputIrina MilisavPeerJ Inc.articlep16Gene expressionHepatocytesHepG2LiverFaOMedicineRENPeerJ, Vol 9, p e12358 (2021) |
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p16 Gene expression Hepatocytes HepG2 Liver FaO Medicine R |
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p16 Gene expression Hepatocytes HepG2 Liver FaO Medicine R Barbara Kramar Dušan Šuput Irina Milisav Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines |
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Background One of the most frequently deleted genes in cancer is CDKN2A encoding p16. This protein is often overexpressed in senescent cells, while its suppression can bypass the oncogene-induced senescence to enable transformation and tumorigenesis. The roles of the protein p16 are recently being expanded from the cell cycle progression regulator to the cellular regulator interacting in several different pathways. Yet data on its liver and liver cells’ expression are inconclusive. Methods The expression of the p16 gene in liver and liver cells was determined by RT-qPCR and compared to its protein amounts by western blotting. Results p16 is expressed at low levels in the liver and rat hepatocytes. Its expression varies from none to the considerable levels in the examined hepatocellular carcinoma cell lines (FaO and HepG2) and in immortalized mouse hepatocytes. Such significant expression differences of an important cellular regulator warrant the need to closely examine the differences in biochemical pathways correlated with the p16 expression when using hepatocytes and hepatoma liver models. |
format |
article |
author |
Barbara Kramar Dušan Šuput Irina Milisav |
author_facet |
Barbara Kramar Dušan Šuput Irina Milisav |
author_sort |
Barbara Kramar |
title |
Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines |
title_short |
Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines |
title_full |
Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines |
title_fullStr |
Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines |
title_full_unstemmed |
Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines |
title_sort |
differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines |
publisher |
PeerJ Inc. |
publishDate |
2021 |
url |
https://doaj.org/article/761dfcb95a51440793c3a9df063a1ddb |
work_keys_str_mv |
AT barbarakramar differentialp16expressionlevelsintheliverhepatocytesandhepatocellularcelllines AT dusansuput differentialp16expressionlevelsintheliverhepatocytesandhepatocellularcelllines AT irinamilisav differentialp16expressionlevelsintheliverhepatocytesandhepatocellularcelllines |
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1718444778158292992 |