Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines

Background One of the most frequently deleted genes in cancer is CDKN2A encoding p16. This protein is often overexpressed in senescent cells, while its suppression can bypass the oncogene-induced senescence to enable transformation and tumorigenesis. The roles of the protein p16 are recently being e...

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Autores principales: Barbara Kramar, Dušan Šuput, Irina Milisav
Formato: article
Lenguaje:EN
Publicado: PeerJ Inc. 2021
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p16
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Acceso en línea:https://doaj.org/article/761dfcb95a51440793c3a9df063a1ddb
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spelling oai:doaj.org-article:761dfcb95a51440793c3a9df063a1ddb2021-11-04T15:05:18ZDifferential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines10.7717/peerj.123582167-8359https://doaj.org/article/761dfcb95a51440793c3a9df063a1ddb2021-11-01T00:00:00Zhttps://peerj.com/articles/12358.pdfhttps://peerj.com/articles/12358/https://doaj.org/toc/2167-8359Background One of the most frequently deleted genes in cancer is CDKN2A encoding p16. This protein is often overexpressed in senescent cells, while its suppression can bypass the oncogene-induced senescence to enable transformation and tumorigenesis. The roles of the protein p16 are recently being expanded from the cell cycle progression regulator to the cellular regulator interacting in several different pathways. Yet data on its liver and liver cells’ expression are inconclusive. Methods The expression of the p16 gene in liver and liver cells was determined by RT-qPCR and compared to its protein amounts by western blotting. Results p16 is expressed at low levels in the liver and rat hepatocytes. Its expression varies from none to the considerable levels in the examined hepatocellular carcinoma cell lines (FaO and HepG2) and in immortalized mouse hepatocytes. Such significant expression differences of an important cellular regulator warrant the need to closely examine the differences in biochemical pathways correlated with the p16 expression when using hepatocytes and hepatoma liver models.Barbara KramarDušan ŠuputIrina MilisavPeerJ Inc.articlep16Gene expressionHepatocytesHepG2LiverFaOMedicineRENPeerJ, Vol 9, p e12358 (2021)
institution DOAJ
collection DOAJ
language EN
topic p16
Gene expression
Hepatocytes
HepG2
Liver
FaO
Medicine
R
spellingShingle p16
Gene expression
Hepatocytes
HepG2
Liver
FaO
Medicine
R
Barbara Kramar
Dušan Šuput
Irina Milisav
Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines
description Background One of the most frequently deleted genes in cancer is CDKN2A encoding p16. This protein is often overexpressed in senescent cells, while its suppression can bypass the oncogene-induced senescence to enable transformation and tumorigenesis. The roles of the protein p16 are recently being expanded from the cell cycle progression regulator to the cellular regulator interacting in several different pathways. Yet data on its liver and liver cells’ expression are inconclusive. Methods The expression of the p16 gene in liver and liver cells was determined by RT-qPCR and compared to its protein amounts by western blotting. Results p16 is expressed at low levels in the liver and rat hepatocytes. Its expression varies from none to the considerable levels in the examined hepatocellular carcinoma cell lines (FaO and HepG2) and in immortalized mouse hepatocytes. Such significant expression differences of an important cellular regulator warrant the need to closely examine the differences in biochemical pathways correlated with the p16 expression when using hepatocytes and hepatoma liver models.
format article
author Barbara Kramar
Dušan Šuput
Irina Milisav
author_facet Barbara Kramar
Dušan Šuput
Irina Milisav
author_sort Barbara Kramar
title Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines
title_short Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines
title_full Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines
title_fullStr Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines
title_full_unstemmed Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines
title_sort differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines
publisher PeerJ Inc.
publishDate 2021
url https://doaj.org/article/761dfcb95a51440793c3a9df063a1ddb
work_keys_str_mv AT barbarakramar differentialp16expressionlevelsintheliverhepatocytesandhepatocellularcelllines
AT dusansuput differentialp16expressionlevelsintheliverhepatocytesandhepatocellularcelllines
AT irinamilisav differentialp16expressionlevelsintheliverhepatocytesandhepatocellularcelllines
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