A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients

Abstract Endometriosis, a major reproductive pathology affecting 8–10% of women is characterized by chronic inflammation and immune dysfunction. Human antigen R (HuR) and Tristetraprolin (TTP) are RNA binding proteins that competitively bind to cytokines involved in inflammation including: tumor nec...

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Autores principales:	Kasra Khalaj, Soo Hyun Ahn, Mallikarjun Bidarimath, Yasmin Nasirzadeh, Sukhbir S. Singh, Asgerally T. Fazleabas, Steven L. Young, Bruce A. Lessey, Madhuri Koti, Chandrakant Tayade
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7624af9b99e044b28c2c2981b2563cdf
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spelling	oai:doaj.org-article:7624af9b99e044b28c2c2981b2563cdf2021-12-02T15:05:27ZA balancing act: RNA binding protein HuR/TTP axis in endometriosis patients10.1038/s41598-017-06081-72045-2322https://doaj.org/article/7624af9b99e044b28c2c2981b2563cdf2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06081-7https://doaj.org/toc/2045-2322Abstract Endometriosis, a major reproductive pathology affecting 8–10% of women is characterized by chronic inflammation and immune dysfunction. Human antigen R (HuR) and Tristetraprolin (TTP) are RNA binding proteins that competitively bind to cytokines involved in inflammation including: tumor necrosis factor alpha (TNF-α), granulocyte macrophage colony stimulating factor (GM-CSF), interleukin 6 (IL-6) among others, and stabilize and destabilize them, respectively. The aim of this study was to examine RNA binding protein (RNABP) HuR/TTP axis in endometriosis patients compared to menstrual stage matched healthy fertile controls in hopes of better understanding their contribution to the pathogenesis of endometriosis. Additionally, using a targeted in vitro siRNA approach, we examined whether knock-down of TTP can play a functional role on other RNABPs that competitively bind to inflammatory targets of TTP in both endometriotic and endometrial epithelial cell lines. Our results suggest that RNABPs TTP and HuR are dysregulated in endometriotic lesions compared to matched eutopic patient samples as well endometrium from healthy controls. Silencing of TTP in endometriotic and endometrial epithelial cells revealed differential response to inflammatory cytokines and other RNABPs. Our results suggest potential involvement of HuR/TTP RNA binding protein axis in regulation of inflammation in endometriosis.Kasra KhalajSoo Hyun AhnMallikarjun BidarimathYasmin NasirzadehSukhbir S. SinghAsgerally T. FazleabasSteven L. YoungBruce A. LesseyMadhuri KotiChandrakant TayadeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kasra Khalaj Soo Hyun Ahn Mallikarjun Bidarimath Yasmin Nasirzadeh Sukhbir S. Singh Asgerally T. Fazleabas Steven L. Young Bruce A. Lessey Madhuri Koti Chandrakant Tayade A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients
description	Abstract Endometriosis, a major reproductive pathology affecting 8–10% of women is characterized by chronic inflammation and immune dysfunction. Human antigen R (HuR) and Tristetraprolin (TTP) are RNA binding proteins that competitively bind to cytokines involved in inflammation including: tumor necrosis factor alpha (TNF-α), granulocyte macrophage colony stimulating factor (GM-CSF), interleukin 6 (IL-6) among others, and stabilize and destabilize them, respectively. The aim of this study was to examine RNA binding protein (RNABP) HuR/TTP axis in endometriosis patients compared to menstrual stage matched healthy fertile controls in hopes of better understanding their contribution to the pathogenesis of endometriosis. Additionally, using a targeted in vitro siRNA approach, we examined whether knock-down of TTP can play a functional role on other RNABPs that competitively bind to inflammatory targets of TTP in both endometriotic and endometrial epithelial cell lines. Our results suggest that RNABPs TTP and HuR are dysregulated in endometriotic lesions compared to matched eutopic patient samples as well endometrium from healthy controls. Silencing of TTP in endometriotic and endometrial epithelial cells revealed differential response to inflammatory cytokines and other RNABPs. Our results suggest potential involvement of HuR/TTP RNA binding protein axis in regulation of inflammation in endometriosis.
format	article
author	Kasra Khalaj Soo Hyun Ahn Mallikarjun Bidarimath Yasmin Nasirzadeh Sukhbir S. Singh Asgerally T. Fazleabas Steven L. Young Bruce A. Lessey Madhuri Koti Chandrakant Tayade
author_facet	Kasra Khalaj Soo Hyun Ahn Mallikarjun Bidarimath Yasmin Nasirzadeh Sukhbir S. Singh Asgerally T. Fazleabas Steven L. Young Bruce A. Lessey Madhuri Koti Chandrakant Tayade
author_sort	Kasra Khalaj
title	A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients
title_short	A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients
title_full	A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients
title_fullStr	A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients
title_full_unstemmed	A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients
title_sort	balancing act: rna binding protein hur/ttp axis in endometriosis patients
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7624af9b99e044b28c2c2981b2563cdf
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A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients

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