<italic toggle="yes">Citrobacter</italic> Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions

ABSTRACT An efficient energy harvesting mechanism is likely critical for animals in their natural environment. Intestinal microbiota enriched by a high-fat diet aid in lipid accumulation, a strategy likely evolved for energy harvest in mammals. However, whether this strategy is conserved among verte...

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Autores principales:	Mei-Ling Zhang, Miao Li, Yi Sheng, Fang Tan, Liqiao Chen, Isaac Cann, Zhen-Yu Du
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	energy harvest gut microbiota high-fat diet fish intestinal permeability Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/762b238c16384074b06acddec0cb4184
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spelling	oai:doaj.org-article:762b238c16384074b06acddec0cb41842021-12-02T18:44:35Z<italic toggle="yes">Citrobacter</italic> Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions10.1128/mSystems.00303-202379-5077https://doaj.org/article/762b238c16384074b06acddec0cb41842020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00303-20https://doaj.org/toc/2379-5077ABSTRACT An efficient energy harvesting mechanism is likely critical for animals in their natural environment. Intestinal microbiota enriched by a high-fat diet aid in lipid accumulation, a strategy likely evolved for energy harvest in mammals. However, whether this strategy is conserved among vertebrate organisms remains unclear. A bacterial strain (S1), enriched on soybean oil rich medium, was isolated from the gut of Nile tilapia and demonstrated to be a member of the Citrobacter genus. Although a high-fat diet increased the number of Citrobacter spp., these bacteria were not abundant in the intestine by high-throughput sequencing. Addition of bacterium S1 to a high-fat diet modulated intestinal microbial composition and increased high-fat diet-induced lipid accumulation in mesenteric adipose tissue, accompanied by (i) increased triglyceride absorption efficiency and triglyceride reesterification and (ii) increased intestinal permeability. Collectively, our results provide evidence that specific intestinal bacteria aid the host in harvesting more energy from a high-fat diet in fish. Furthermore, the results from the present study also suggest that nondominant bacteria in the gut may play an important role in regulating host metabolism. IMPORTANCE This study shows that the ability of gut microbiota members to enhance host energy harvest from a high-fat diet is a conserved feature of host-microbe interactions in fish, as in mammals. It also underscores that gut microbiota members are able to significantly impact host biology even when at low abundance.Mei-Ling ZhangMiao LiYi ShengFang TanLiqiao ChenIsaac CannZhen-Yu DuAmerican Society for Microbiologyarticleenergy harvestgut microbiotahigh-fat dietfishintestinal permeabilityMicrobiologyQR1-502ENmSystems, Vol 5, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	energy harvest gut microbiota high-fat diet fish intestinal permeability Microbiology QR1-502
spellingShingle	energy harvest gut microbiota high-fat diet fish intestinal permeability Microbiology QR1-502 Mei-Ling Zhang Miao Li Yi Sheng Fang Tan Liqiao Chen Isaac Cann Zhen-Yu Du <italic toggle="yes">Citrobacter</italic> Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions
description	ABSTRACT An efficient energy harvesting mechanism is likely critical for animals in their natural environment. Intestinal microbiota enriched by a high-fat diet aid in lipid accumulation, a strategy likely evolved for energy harvest in mammals. However, whether this strategy is conserved among vertebrate organisms remains unclear. A bacterial strain (S1), enriched on soybean oil rich medium, was isolated from the gut of Nile tilapia and demonstrated to be a member of the Citrobacter genus. Although a high-fat diet increased the number of Citrobacter spp., these bacteria were not abundant in the intestine by high-throughput sequencing. Addition of bacterium S1 to a high-fat diet modulated intestinal microbial composition and increased high-fat diet-induced lipid accumulation in mesenteric adipose tissue, accompanied by (i) increased triglyceride absorption efficiency and triglyceride reesterification and (ii) increased intestinal permeability. Collectively, our results provide evidence that specific intestinal bacteria aid the host in harvesting more energy from a high-fat diet in fish. Furthermore, the results from the present study also suggest that nondominant bacteria in the gut may play an important role in regulating host metabolism. IMPORTANCE This study shows that the ability of gut microbiota members to enhance host energy harvest from a high-fat diet is a conserved feature of host-microbe interactions in fish, as in mammals. It also underscores that gut microbiota members are able to significantly impact host biology even when at low abundance.
format	article
author	Mei-Ling Zhang Miao Li Yi Sheng Fang Tan Liqiao Chen Isaac Cann Zhen-Yu Du
author_facet	Mei-Ling Zhang Miao Li Yi Sheng Fang Tan Liqiao Chen Isaac Cann Zhen-Yu Du
author_sort	Mei-Ling Zhang
title	<italic toggle="yes">Citrobacter</italic> Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions
title_short	<italic toggle="yes">Citrobacter</italic> Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions
title_full	<italic toggle="yes">Citrobacter</italic> Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions
title_fullStr	<italic toggle="yes">Citrobacter</italic> Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions
title_full_unstemmed	<italic toggle="yes">Citrobacter</italic> Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions
title_sort	<italic toggle="yes">citrobacter</italic> species increase energy harvest by modulating intestinal microbiota in fish: nondominant species play important functions
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/762b238c16384074b06acddec0cb4184
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<italic toggle="yes">Citrobacter</italic> Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions

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