Anti-Obesity Effect of α-Cubebenol Isolated from <i>Schisandra chinensis</i> in 3T3-L1 Adipocytes

The efficacy of α-cubebenol isolated from <i>Schisandra chinensis</i> has been studied in several diseases, including cecal ligation, puncture challenge-induced sepsis, and degranulation of neutrophils. To identify the novel functions of α-cubebenol on lipid metabolism, alterations on th...

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Autores principales:	Su Jin Lee, Ji Eun Kim, Yun Ju Choi, Jeong Eun Gong, You Jeong Jin, Da Woon Lee, Young Whan Choi, Dae Youn Hwang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	α-cubebenol obesity lipogenesis β-oxidation lipolysis inflammasome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/762b7da63876452aaf407a9ed278a4a6
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spelling	oai:doaj.org-article:762b7da63876452aaf407a9ed278a4a62021-11-25T16:53:23ZAnti-Obesity Effect of α-Cubebenol Isolated from <i>Schisandra chinensis</i> in 3T3-L1 Adipocytes10.3390/biom111116502218-273Xhttps://doaj.org/article/762b7da63876452aaf407a9ed278a4a62021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1650https://doaj.org/toc/2218-273XThe efficacy of α-cubebenol isolated from <i>Schisandra chinensis</i> has been studied in several diseases, including cecal ligation, puncture challenge-induced sepsis, and degranulation of neutrophils. To identify the novel functions of α-cubebenol on lipid metabolism, alterations on the regulation of lipogenesis, lipolysis, and inflammatory response were observed in 3T3-L1 adipocytes treated with α-cubebenol. Most lipogenic targets, including lipid accumulation, level of lipogenic transcription factors, and expression of lipogenic regulators, were suppressed in MDI (3-isobutyl-1-methylxanthine, dexamethasone, and insulin)-stimulated 3T3-L1 adipocytes treated with α-cubebenol without significant cytotoxicity. In addition, similar inhibition effects were observed in the iNOS-induced COX-2 mediated pathway and NLRP3 inflammasome pathway of MDI-stimulated 3T3-L1 cells treated with α-cubebenol. Lipolytic targets, such as cAMP concentration, expression of adenylyl cyclase and PDE4, and their downstream signaling pathway, in MDI-stimulated 3T3-L1 cells were stimulated by the α-cubebenol treatment. The levels of transcription factors and related proteins for β-oxidation were significantly higher in the MDI + α-cubebenol treated group than in the MDI + Vehicle treated group. These results show that α-cubebenol has a novel role as a lipogenesis inhibitor, lipolysis and β-oxidation stimulator, and inflammasome suppressor in MDI-stimulated 3T3-L1 adipocytes.Su Jin LeeJi Eun KimYun Ju ChoiJeong Eun GongYou Jeong JinDa Woon LeeYoung Whan ChoiDae Youn HwangMDPI AGarticleα-cubebenolobesitylipogenesisβ-oxidationlipolysisinflammasomeMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1650, p 1650 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	α-cubebenol obesity lipogenesis β-oxidation lipolysis inflammasome Microbiology QR1-502
spellingShingle	α-cubebenol obesity lipogenesis β-oxidation lipolysis inflammasome Microbiology QR1-502 Su Jin Lee Ji Eun Kim Yun Ju Choi Jeong Eun Gong You Jeong Jin Da Woon Lee Young Whan Choi Dae Youn Hwang Anti-Obesity Effect of α-Cubebenol Isolated from <i>Schisandra chinensis</i> in 3T3-L1 Adipocytes
description	The efficacy of α-cubebenol isolated from <i>Schisandra chinensis</i> has been studied in several diseases, including cecal ligation, puncture challenge-induced sepsis, and degranulation of neutrophils. To identify the novel functions of α-cubebenol on lipid metabolism, alterations on the regulation of lipogenesis, lipolysis, and inflammatory response were observed in 3T3-L1 adipocytes treated with α-cubebenol. Most lipogenic targets, including lipid accumulation, level of lipogenic transcription factors, and expression of lipogenic regulators, were suppressed in MDI (3-isobutyl-1-methylxanthine, dexamethasone, and insulin)-stimulated 3T3-L1 adipocytes treated with α-cubebenol without significant cytotoxicity. In addition, similar inhibition effects were observed in the iNOS-induced COX-2 mediated pathway and NLRP3 inflammasome pathway of MDI-stimulated 3T3-L1 cells treated with α-cubebenol. Lipolytic targets, such as cAMP concentration, expression of adenylyl cyclase and PDE4, and their downstream signaling pathway, in MDI-stimulated 3T3-L1 cells were stimulated by the α-cubebenol treatment. The levels of transcription factors and related proteins for β-oxidation were significantly higher in the MDI + α-cubebenol treated group than in the MDI + Vehicle treated group. These results show that α-cubebenol has a novel role as a lipogenesis inhibitor, lipolysis and β-oxidation stimulator, and inflammasome suppressor in MDI-stimulated 3T3-L1 adipocytes.
format	article
author	Su Jin Lee Ji Eun Kim Yun Ju Choi Jeong Eun Gong You Jeong Jin Da Woon Lee Young Whan Choi Dae Youn Hwang
author_facet	Su Jin Lee Ji Eun Kim Yun Ju Choi Jeong Eun Gong You Jeong Jin Da Woon Lee Young Whan Choi Dae Youn Hwang
author_sort	Su Jin Lee
title	Anti-Obesity Effect of α-Cubebenol Isolated from <i>Schisandra chinensis</i> in 3T3-L1 Adipocytes
title_short	Anti-Obesity Effect of α-Cubebenol Isolated from <i>Schisandra chinensis</i> in 3T3-L1 Adipocytes
title_full	Anti-Obesity Effect of α-Cubebenol Isolated from <i>Schisandra chinensis</i> in 3T3-L1 Adipocytes
title_fullStr	Anti-Obesity Effect of α-Cubebenol Isolated from <i>Schisandra chinensis</i> in 3T3-L1 Adipocytes
title_full_unstemmed	Anti-Obesity Effect of α-Cubebenol Isolated from <i>Schisandra chinensis</i> in 3T3-L1 Adipocytes
title_sort	anti-obesity effect of α-cubebenol isolated from <i>schisandra chinensis</i> in 3t3-l1 adipocytes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/762b7da63876452aaf407a9ed278a4a6
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Anti-Obesity Effect of α-Cubebenol Isolated from <i>Schisandra chinensis</i> in 3T3-L1 Adipocytes

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