Avian influenza outbreaks: evaluating the efficacy of cleaning and disinfection of vehicles and transport crates
ABSTRACT: In 2021, France faced large avian influenza outbreaks, like in 2016 and 2017. Controlling these outbreaks required the preventive depopulation of a large number of duck farms. A previous study in 2017 showed that the quality of decontamination of trucks and transport crates used for depopu...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Elsevier
2022
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/7637c286b64942efabe64fa29bea8d3d |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:7637c286b64942efabe64fa29bea8d3d |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:7637c286b64942efabe64fa29bea8d3d2021-11-24T04:23:26ZAvian influenza outbreaks: evaluating the efficacy of cleaning and disinfection of vehicles and transport crates0032-579110.1016/j.psj.2021.101569https://doaj.org/article/7637c286b64942efabe64fa29bea8d3d2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005903https://doaj.org/toc/0032-5791ABSTRACT: In 2021, France faced large avian influenza outbreaks, like in 2016 and 2017. Controlling these outbreaks required the preventive depopulation of a large number of duck farms. A previous study in 2017 showed that the quality of decontamination of trucks and transport crates used for depopulation was often insufficient. A new study was then set up to evaluate cleaning and disinfection (C&D) of trucks and crates used for duck depopulation and whether practices had changed since 2017. Three methods were used to assess decontamination: 1) detection of avian influenza virus (AIV) genome, 2) visual inspection of cleanliness, and 3) microbial counts, considering that 2 and 3 are commonly used in abattoirs. Another objective of the study was to evaluate the correlation between results obtained with the 3 methods. In 5 abattoirs, 8 trucks and their crates were sampled by swabbing to detect AIV genome by rRT-PCR before and after decontamination. Visual cleanliness scores and coliform counts were also determined on crates after C&D. Trucks and crates were decontaminated according to the abattoirs’ protocols. Before C&D, 3 quarters of crates (59/79) and 7 of 8 trucks were positive for AIV genome. C&D procedures were reinforced in 2021 compared to 2017; use of detergent solution and warm water were more common. Nevertheless, 28% of the crates were positive for AIV genome after C&D, despite the fact that cleaning scores and microbiological counts were satisfactory for 84% and 91% of the crates, respectively. No correlation was observed between results for AIV genome detection and results from visual control or from coliform counts. Abattoirs are encouraged to use environmental sampling coupled with AIV genome detection to monitor the quality of cleaning and disinfection of trucks and crates during AI outbreaks. Reinforcement of biosecurity measures at abattoirs is still needed to avoid residual contamination of the equipment and cross-contamination during the decontamination process.Adeline Huneau-SalaünAxelle ScoizecRodolphe ThomasClaire MartenotAudrey SchmitzIsabelle PierreChantal AlléeRachel BussonPascale MassinFrançois-Xavier BriandCarole GuillemotoKatell LouboutinFlorent SouchaudMartine Cherbonnel-PansartEric NiqueuxBéatrice GraslandRozenn SouillardSophie Le BouquinElsevierarticleavian influenzatransportcleaningdisinfectionbiosecurityAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101569- (2022) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
avian influenza transport cleaning disinfection biosecurity Animal culture SF1-1100 |
| spellingShingle |
avian influenza transport cleaning disinfection biosecurity Animal culture SF1-1100 Adeline Huneau-Salaün Axelle Scoizec Rodolphe Thomas Claire Martenot Audrey Schmitz Isabelle Pierre Chantal Allée Rachel Busson Pascale Massin François-Xavier Briand Carole Guillemoto Katell Louboutin Florent Souchaud Martine Cherbonnel-Pansart Eric Niqueux Béatrice Grasland Rozenn Souillard Sophie Le Bouquin Avian influenza outbreaks: evaluating the efficacy of cleaning and disinfection of vehicles and transport crates |
| description |
ABSTRACT: In 2021, France faced large avian influenza outbreaks, like in 2016 and 2017. Controlling these outbreaks required the preventive depopulation of a large number of duck farms. A previous study in 2017 showed that the quality of decontamination of trucks and transport crates used for depopulation was often insufficient. A new study was then set up to evaluate cleaning and disinfection (C&D) of trucks and crates used for duck depopulation and whether practices had changed since 2017. Three methods were used to assess decontamination: 1) detection of avian influenza virus (AIV) genome, 2) visual inspection of cleanliness, and 3) microbial counts, considering that 2 and 3 are commonly used in abattoirs. Another objective of the study was to evaluate the correlation between results obtained with the 3 methods. In 5 abattoirs, 8 trucks and their crates were sampled by swabbing to detect AIV genome by rRT-PCR before and after decontamination. Visual cleanliness scores and coliform counts were also determined on crates after C&D. Trucks and crates were decontaminated according to the abattoirs’ protocols. Before C&D, 3 quarters of crates (59/79) and 7 of 8 trucks were positive for AIV genome. C&D procedures were reinforced in 2021 compared to 2017; use of detergent solution and warm water were more common. Nevertheless, 28% of the crates were positive for AIV genome after C&D, despite the fact that cleaning scores and microbiological counts were satisfactory for 84% and 91% of the crates, respectively. No correlation was observed between results for AIV genome detection and results from visual control or from coliform counts. Abattoirs are encouraged to use environmental sampling coupled with AIV genome detection to monitor the quality of cleaning and disinfection of trucks and crates during AI outbreaks. Reinforcement of biosecurity measures at abattoirs is still needed to avoid residual contamination of the equipment and cross-contamination during the decontamination process. |
| format |
article |
| author |
Adeline Huneau-Salaün Axelle Scoizec Rodolphe Thomas Claire Martenot Audrey Schmitz Isabelle Pierre Chantal Allée Rachel Busson Pascale Massin François-Xavier Briand Carole Guillemoto Katell Louboutin Florent Souchaud Martine Cherbonnel-Pansart Eric Niqueux Béatrice Grasland Rozenn Souillard Sophie Le Bouquin |
| author_facet |
Adeline Huneau-Salaün Axelle Scoizec Rodolphe Thomas Claire Martenot Audrey Schmitz Isabelle Pierre Chantal Allée Rachel Busson Pascale Massin François-Xavier Briand Carole Guillemoto Katell Louboutin Florent Souchaud Martine Cherbonnel-Pansart Eric Niqueux Béatrice Grasland Rozenn Souillard Sophie Le Bouquin |
| author_sort |
Adeline Huneau-Salaün |
| title |
Avian influenza outbreaks: evaluating the efficacy of cleaning and disinfection of vehicles and transport crates |
| title_short |
Avian influenza outbreaks: evaluating the efficacy of cleaning and disinfection of vehicles and transport crates |
| title_full |
Avian influenza outbreaks: evaluating the efficacy of cleaning and disinfection of vehicles and transport crates |
| title_fullStr |
Avian influenza outbreaks: evaluating the efficacy of cleaning and disinfection of vehicles and transport crates |
| title_full_unstemmed |
Avian influenza outbreaks: evaluating the efficacy of cleaning and disinfection of vehicles and transport crates |
| title_sort |
avian influenza outbreaks: evaluating the efficacy of cleaning and disinfection of vehicles and transport crates |
| publisher |
Elsevier |
| publishDate |
2022 |
| url |
https://doaj.org/article/7637c286b64942efabe64fa29bea8d3d |
| work_keys_str_mv |
AT adelinehuneausalaun avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT axellescoizec avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT rodolphethomas avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT clairemartenot avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT audreyschmitz avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT isabellepierre avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT chantalallee avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT rachelbusson avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT pascalemassin avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT francoisxavierbriand avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT caroleguillemoto avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT katelllouboutin avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT florentsouchaud avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT martinecherbonnelpansart avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT ericniqueux avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT beatricegrasland avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT rozennsouillard avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates AT sophielebouquin avianinfluenzaoutbreaksevaluatingtheefficacyofcleaninganddisinfectionofvehiclesandtransportcrates |
| _version_ |
1718416041166503936 |